Obtaining hybrids of cultivars and wild subspecies of cassava

Silva, Dreid de Cerqueira Silveira da; Martins, Márcio Lacerda Lopes; Santos, Ariana Silva; Santos, V. da S.; Alves, Alfredo Augusto Cunha; Lêdo, Carlos Alberto da Silva

doi:10.1590/s0100-204x2018000200006

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…It has been suggested that fruit abortion in out-crossing species may be due to deleterious mutations (Wiens et al, 1987). Multiple studies have shown low pollen amounts and low pollination rates in cultivated cassava crosses compared to wild progenitors and other Manihot species (da Silva et al, 2018; Jennings, n.d.; Vieira et al, 2012). Previous studies on the relationship between cassava clonality on deleterious mutations have shown an unexpected lack of correlation between recombination and deleterious mutations (Ramu et al 2017), supporting the conclusion that these mutations are indeed being enriched through absent recombination from clonal reproduction.…”

Section: Discussionmentioning

confidence: 99%

Muller’s Ratchet in Action: The Erosion of Sexual Reproduction Genes in Domesticated Cassava (Manihot esculenta)

Long,

Stitzer,

Monier

et al. 2024

Preprint

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Centuries of clonal propagation in cassava (Manihot esculenta) have engaged Muller's Ratchet, leading to the accumulation of deleterious mutations due to the absence of sexual recombination. This has resulted in both inbreeding depression affecting yield and a significant decrease in reproductive performance, creating hurdles for contemporary breeding programs. Cassava is a member of the Euphorbiaceae family, including notable species such as rubber tree (Hevea brasiliensis) and poinsettia (Euphorbia pulcherrima). Expanding upon preliminary draft genomes, we annotated 7 long-read genome assemblies and aligned a total of 52 genomes, to analyze selection across the genome and the phylogeny. Through this comparative genomic approach, we identified 48 genes under relaxed selection in cassava. Notably, we discovered an overrepresentation of floral expressed genes, especially focused at six pollen-related genes. Our results indicate that domestication and a transition to clonal propagation has reduced selection pressures on sexually reproductive functions in cassava leading to an accumulation of mutations in pollen-related genes. This relaxed selection and the genome-wide deleterious mutations responsible for inbreeding depression are potential targets for improving cassava breeding, where the generation of new varieties relies on recombining favorable alleles through sexual reproduction.

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Section: Discussionmentioning

confidence: 99%

Muller’s Ratchet in Action: The Erosion of Sexual Reproduction Genes in Domesticated Cassava (Manihot esculenta)

Long,

Stitzer,

Monier

et al. 2024

Preprint

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“…Parental reconstruction has also been successfully done in other root and tuber crops such as cassava (Manihot esculenta Crantz) [40][41][42], potato (Solanum tuberosum L.) [43,44] and sweetpotato (Ipomoea batatas L.) [45]. In these crops, molecular parentage analysis facilitated the discovery of pedigree errors or mismatches in populations improved using natural and artificial mating schemes [44,46,47].…”

Section: Allelic Diversity and Parental Reconstructionmentioning

confidence: 99%

Paternity Assignment in White Guinea Yam (Dioscorea Rotundata) Half-Sib Progenies from Polycross Mating Design Using SNP Markers

Norman

Agre

Danquah

et al. 2020

Plants

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White Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generating variability in yam breeding. However, the pollen parent of progeny from polycross mating is usually unknown. This study aimed to determine paternity in white Guinea yam half-sib progenies from polycross mating design. A total of 394 half-sib progenies from random open pollination involving nine female and three male parents was genotyped with 6602 SNP markers from DArTSeq platform to recover full pedigree. A higher proportion of expected heterozygosity, allelic richness, and evenness were observed in the half-sib progenies. A complete pedigree was established for all progenies from two families (TDr1685 and TDr1688) with 100% accuracy, while in the remaining families, paternity was assigned successfully only for 56 to 98% of the progenies. Our results indicated unequal paternal contribution under natural open pollination in yam, suggesting unequal pollen migrations or gene flow among the crossing parents. A total of 3.8% of progenies lacking paternal identity due to foreign pollen contamination outside the polycross block was observed. This study established the efficient determination of parental reconstruction and allelic contributions in the white Guinea yam half-sib progenies generated from open pollination polycross using SNP markers. Findings are useful for parental reconstruction, accurate dissection of the genetic effects, and selection in white Guinea yam breeding program utilizing polycross mating design.

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“…In root and tuber crops, microsatellites have been utilized to determine the progeny-paternity relationship (Table 1). Microsatellites have been used for parentage analysis in yams [32][33][34][35][36][37][38][39], potatoes [44][45][46][48][49][50]52,53,55], cassava [63][64][65][66][67][68][69][70][71][72][73] and sweet potato [76][77][78][79][80][81][82]84,85].…”

Section: Molecular Marker Systemsmentioning

confidence: 99%

“…This method is not efficient for high quality true seed production, identification of parents that PubMed [20,[64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79][80] Sweet potato (Ipomoea batatas L.) Root Google Scholar, Springer Link 11 [81][82][83][84][85][86][87][88][89][90][91] …”

Section: Introductionmentioning

confidence: 99%

Can Parentage Analysis Facilitate Breeding Activities in Root and Tuber Crops?

et al. 2018

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Controlled pollination in root and tuber crops is challenging. Complex ploidy, cross-incompatibility, erratic flowering patterns, outcrossing, etc., limit the efficiency of breeding progress in these crops. Half-sib breeding that involves random pollination among parents is a viable method to harness genetic gain in outcrossing crops that are problematic for performing planned and controlled pollination. The authenticity of resulting progenies from the half-sib breeding is essential to monitor the selection gain in the breeding program. Parentage analysis facilitated by molecular markers is among the available handy tools for crop breeders to maximize genetic gain in a breeding program. It can help to resolve the identity of half-sib progenies and reconstruct the pedigree in the outcrossing crops. This paper reviews the potential benefits of parentage analysis in breeding selected outcrossing root and tuber crops. It assesses how paternity analysis facilitates breeding activities and the ways it improves genetic gain in the root and tuber breeding programs. Conscious use of complementary techniques in the root and tuber breeding programs can increase the selection gain by reducing the long breeding cycle and cost, as well as reliable exploitation of the heritable variation in the desired direction.

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Obtaining hybrids of cultivars and wild subspecies of cassava

Cited by 5 publications

References 20 publications

Muller’s Ratchet in Action: The Erosion of Sexual Reproduction Genes in Domesticated Cassava (Manihot esculenta)

Muller’s Ratchet in Action: The Erosion of Sexual Reproduction Genes in Domesticated Cassava (Manihot esculenta)

Paternity Assignment in White Guinea Yam (Dioscorea Rotundata) Half-Sib Progenies from Polycross Mating Design Using SNP Markers

Can Parentage Analysis Facilitate Breeding Activities in Root and Tuber Crops?

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