A large amount of eggplant straw (ES) is incinerated after harvesting of eggplant every year, which aggravates environmental pollution and waste of resources. Converting ES into methane through anaerobic digestion (AD) technology may be a potential treatment method, considering the low environmental impact and high energy recovery. Firstly, this study explored the effects of organic loading (OL) and feed to inoculum ratio (F/I ratio) on the AD of ES by response surface methodology (RSM). In order to achieve higher AD efficiency, various pretreatments (acid, alkali, alkaline hydrogen peroxide (AHP), microwave and ultrasound) were introduced and comprehensively assessed with regard to methane production performance, organic matter destruction and kinetic parameters. Results showed that OL had a more significant impact on AD process compared to F/I ratio and methane production was enhanced remarkably when the OL and F/I ratio were 35.0 g VS/L and 3.0, respectively. XRD, FTIR, and SEM analyses of pretreated ES showed that alkali and AHP pretreatments performed better in delignification. Under optimal conditions, the ES pretreated with 1.5% AHP (adjusted by KOH) performed the maximum methane production of 262.2 mL/g VS with a Bd of 95.0%, which increased by 334.1% than untreated ES. This paper not only provides the theoretical data about methane production performance of ES but also gives practical guidance for efficient utilization of similar vegetable straw biowastes, which is also promising for large-scale industrial applications in the future.