Obtaining of Zinc Oxide Nanoparticles Modified With Galactose and Assessment of Their Cytotoxic Properties

Abstract

The work presents the method for preparation of zinc oxide nanoparticles with galactose coating. Bare zinc oxide nanoparticles are well-known and popular drug delivers. However, the fact that zinc ions may be released from their structure easily, they may pose a threat to the living organism. Thus the modification of such a product has been performed. The physicochemical properties of the products have been analysed. XRD technique revealed crystallographic structure of the products. Based on ATR-FTIR analysis it was confirmed that galactose has been successfully attached to the zinc oxide nanoparticles. TEM-EDS microscopy was applied in order to assess the shape of nanoparticles and also for the confirmation of galactose on the particles surface. The releasing of zinc ions from the modified products was compared to their releasing from basic, non-modified sample. Also, cytotoxicity and proliferation of obtained products have been analysed with using Chinese hamster ovary cells. It was found out that the proposed technology may lead to obtain stable forms of modified zinc oxide nanoparticles with limited toxicity.