Obtaining the transgenic lines of finger millet Eleusine coracana (L.). with dinitroaniline resistance

Bayer, G. Ya.; Yemets, А. І.; Blume, Ya. B.

doi:10.3103/s0095452714030025

Cited by 27 publications

(8 citation statements)

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“…The first well-known α-tubulin mutation of Thr-239-Ile in plants that confers resistance to dinitroaniline herbicides was confirmed in transgenic maize calli ( Anthony et al, 1998 ; Anthony and Hussey, 1999 ). Regenerable transgenic tobacco and finger millet ( Eleusine coracana ) seedlings overexpressing recombinant resistant α- and wild-type β-tubulins that are resistant to dinitroanilines have been obtained ( Anthony et al, 1999 ; Bayer et al, 2014 ), demonstrating the relationship between the calli transformation and plant expression of the trangene. In the current study, we have successfully transformed rice calli with a mutant L. rigidum α-tubulin gene together with a wild-type rice β-tubulin gene.…”

Section: Discussionmentioning

confidence: 99%

Novel α-Tubulin Mutations Conferring Resistance to Dinitroaniline Herbicides in Lolium rigidum

et al. 2018

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The dinitroaniline herbicides (particularly trifluralin) have been globally used in many crops for selective grass weed control. Consequently, trifluralin resistance has been documented in several important crop weed species and has recently reached a level of concern in Australian Lolium rigidum populations. Here, we report novel mutations in the L. rigidum α-tubulin gene which confer resistance to trifluralin and other dinitroaniline herbicides. Nucleotide mutations at the highly conserved codon Arg-243 resulted in amino acid substitutions of Met or Lys. Rice calli transformed with the mutant 243-Met or 243-Lys α-tubulin genes were 4- to 8-fold more resistant to trifluralin and other dinitroaniline herbicides (e.g., ethalfluralin and pendimethalin) compared to calli transformed with the wild type α-tubulin gene from L. rigidum. Comprehensive modeling of molecular docking predicts that Arg-243 is close to the trifluralin binding site on the α-tubulin surface and that replacement of Arg-243 by Met/Lys-243 results in a spatial shift of the trifluralin binding domain, reduction of trifluralin-tubulin contacts, and unfavorable interactions. The major effect of these substitutions is a significant rise of free interaction energy between α-tubulin and trifluralin, as well as between trifluralin and its whole molecular environment. These results demonstrate that the Arg-243 residue in α-tubulin is a determinant for trifluralin sensitivity, and the novel Arg-243-Met/Lys mutations may confer trifluralin resistance in L. rigidum.

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Section: Discussionmentioning

confidence: 99%

Novel α-Tubulin Mutations Conferring Resistance to Dinitroaniline Herbicides in Lolium rigidum

et al. 2018

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“…Using the previously confirmed CKX -deficient lines of E. coracana somaclonal mutants ( Radchuk et al, 2012 ; Bayer et al, 2014 ), we performed an expression profiling of EcCKX and EcCKX1.A / B genes, in particular of EcCKX2.A / B ( Figure 7 ). As mentioned above, homeologous pairs of EcCKX1.A / B and EcCKX2.A / B encode highly conserved peptide products with sequence similarities up to 97.7% and 98.85%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification and analysis of the cytokinin oxidase/dehydrogenase (ckx) gene family in finger millet (Eleusine coracana)

Blume

Yemets²,

Korkhovyi³

et al. 2022

Front. Genet.

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Cytokinin dehydrogenase/oxidase (CKX) enzymes play a key role in regulating cytokinin (CK) levels in plants by degrading the excess of this phytohormone. CKX genes have proven an attractive target for genetic engineering, as their silencing boosts cytokinin accumulation in various tissues, thereby contributing to a rapid increase in biomass and overall plant productivity. We previously reported a similar effect in finger millet (Eleusine coracana) somaclonal lines, caused by downregulation of EcCKX1 and EcCKX2. However, the CKX gene family has numerous representatives, especially in allopolyploid crop species, such as E. coracana. To date, the entire CKX gene family of E. coracana and its related species has not been characterized. We offer here, for the first time, a comprehensive genome-wide identification and analysis of a panel of CKX genes in finger millet. The functional genes identified in the E. coracana genome are compared with the previously-identified genes, EcCKX1 and EcCKX2. Exon-intron structural analysis and motif analysis of FAD- and CK-binding domains are performed. The phylogeny of the EcCKX genes suggests that CKX genes are divided into several distinct groups, corresponding to certain isotypes. Finally, the phenotypic effect of EcCKX1 and EcCKX2 in partially silencing the SE7 somaclonal line is investigated, showing that lines deficient in CKX-expression demonstrate increased grain yield and greater bushiness, enhanced biomass accumulation, and a shorter vegetation cycle.

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“…The first finger millet transformation study was reported by Gupta et al [57], whose preliminary work used biolistic method to compare the efficiency of five gene promoters for the expression of GUS reporter gene. Later, several studies in finger millet focused on development of transgenics for leaf blast resistance [73,92], drought and salinity tolerance [66], herbicide resistance [15], and improved zinc concentration [142]. In foxtail millet, Agrobacterium-mediated transformation was first reported by Liu et al [106].…”

Section: Genetic Transformation Of Small Milletsmentioning

confidence: 99%

Genetic and genomic resources, and breeding for accelerating improvement of small millets: current status and future interventions

Vetriventhan

Azevedo

Upadhyaya

et al. 2020

Nucleus

117

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Current agricultural and food systems encourage research and development on major crops, neglecting regionally important minor crops. Small millets include a group of small-seeded cereal crops of the grass family Poaceae. This includes finger millet, foxtail millet, proso millet, barnyard millet, kodo millet, little millet, teff, fonio, job's tears, guinea millet, and browntop millet. Small millets are an excellent choice to supplement major staple foods for crop and dietary diversity because of their diverse adaptation on marginal lands, less water requirement, lesser susceptibility to stresses, and nutritional superiority compared to major cereal staples. Growing interest among consumers about healthy diets together with climate-resilient features of small millets underline the necessity of directing more research and development towards these crops. Except for finger millet and foxtail millet, and to some extent proso millet and teff, other small millets have received minimal research attention in terms of development of genetic and genomic resources and breeding for yield enhancement. Considerable breeding efforts were made in finger millet and foxtail millet in India and China, respectively, proso millet in the United States of America, and teff in Ethiopia. So far, five genomes, namely foxtail millet, finger millet, proso millet, teff, and Japanese barnyard millet, have been sequenced, and genome of foxtail millet is the smallest (423-510 Mb) while the largest one is finger millet (1.5 Gb). Recent advances in phenotyping and genomics technologies, together with available germplasm diversity, could be utilized in small millets improvement. This review provides a comprehensive insight into the importance of small millets, the global status of their germplasm, diversity, promising germplasm resources, and breeding approaches (conventional and genomic approaches) to accelerate climate-resilient and nutrient-dense small millets for sustainable agriculture, environment, and healthy food systems.

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Obtaining the transgenic lines of finger millet Eleusine coracana (L.). with dinitroaniline resistance

Cited by 27 publications

References 27 publications

Novel α-Tubulin Mutations Conferring Resistance to Dinitroaniline Herbicides in Lolium rigidum

Novel α-Tubulin Mutations Conferring Resistance to Dinitroaniline Herbicides in Lolium rigidum

Genome-wide identification and analysis of the cytokinin oxidase/dehydrogenase (ckx) gene family in finger millet (Eleusine coracana)

Genetic and genomic resources, and breeding for accelerating improvement of small millets: current status and future interventions

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