Occult Lymph Node Metastasis from Desmoplastic Small Round Cell Tumor Diagnosed by Fine Needle Aspiration Cytology

Zeppa, Pio; Lepore, Maria; Vetrani, A; Palombini, Lucio

doi:10.1159/000326559

Cited by 14 publications

(23 citation statements)

References 12 publications

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“…The cytological features that we found in the smears obtained by FNA are similar to other descriptions in the literature. Similar to reports of Zeppa et al [11], we did not detect in our smears fragments of fibrosis or cytoplasmic granules or vacuoles. The finding of stromal fragments, frequently seen in FNA is not a common finding in liquid based preparations [12].…”

Section: Discussionsupporting

confidence: 92%

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

et al. 2005

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BackgroundDesmoplastic small round cell tumor (DSRCT) is a rare and highly aggressive neoplasm. The cytological diagnosis of these tumors can be difficult because they show morphological features quite similar to other small round blue cells tumors. We described four cases of DSRCT with cytological sampling: one obtained by fine needle aspiration biopsy (FNAB) and three from serous effusions. The corresponding immunocytochemical panel was also reviewed.MethodsPapanicolaou stained samples from FNAB and effusions were morphologically described. Immunoreaction with WT1 antibody was performed in all cytological samples. An immunohistochemical panel including the following antibodies was performed in the corresponding biopsies: 34BE12, AE1/AE3, Chromogranin A, CK20, CK7, CK8, Desmin, EMA, NSE, Vimentin and WT1.ResultsThe smears showed high cellularity with minor size alteration. Nuclei were round to oval, some of them with inconspicuous nucleoli. Tumor cells are clustered, showing rosette-like feature. Tumor cells in effusions and FNA were positive to WT1 in 3 of 4 cytology specimens (2 out 3 effusions and one FNA). Immunohistochemical reactions for vimentin, NSE, AE1/AE3 and WT1 were positive in all cases in tissue sections.ConclusionThe use of an adjunct immunocytochemical panel coupled with the cytomorphological characteristics allows the diagnosis of DSRCT in cytological specimens.

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Section: Discussionsupporting

confidence: 92%

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

et al. 2005

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“…12 In similar circumstances, immunohistochemistry (IHC) has been successfully applied to FNABs from other organs to obtain a definitive diagnosis. [23][24][25][26][27] To date, there are relatively few reports in the literature describing the use of IHC on FNABs of ophthalmic lesions. 11,12,28,29 A limiting factor with intraocular aspirates is that there is often little material on which to perform IHC staining.…”

Section: Fine Needle Aspiration Biopsy With Adjunct Immunohistochemismentioning

confidence: 99%

“…15 Immunohistochemistry is now a routine component of both the histologic and cytologic assessment of specimens from a wide variety of anatomic sites. [23][24][25][26][27][43][44][45] However, there is more limited literature describing the use of IHC on ocular cytology samples. In one of the earliest reports, 29 herpes simplex keratitis was diagnosed cytologically in Papanicolaou-stained samples and typed as herpesvirus 1 by IHC on destained cells.…”

Section: A B C Dmentioning

confidence: 99%

Fine Needle Aspiration Biopsy with Adjunct Immunohistochemistry in Intraocular Tumor Management

et al. 2005

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“…Despite the fact that DSRCT is now a well-documented entity with several cytological reports in the literature, generally comprising case reports that include findings in both FNA material and ascitic/pleural effusions [6,7,8,9,17,18,19,20,21,22], its diagnosis is still usually established by histopathological examination.…”

Section: Discussionmentioning

confidence: 99%

Desmoplastic Small Round Cell Tumor: Diagnosis by Fine-Needle Aspiration Cytology

Leça

Vieira²,

Teixeira³

et al. 2012

Acta Cytologica

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Background: Desmoplastic small round cell tumor (DSRCT) is a distinctive clinicopathologic entity with an aggressive clinical course that typically involves the abdominal and/or pelvic peritoneum of young males. A population of small round blue cells and a fibroesclerotic stroma are the usual morphologic features. This tumor is characterized by a typical polyphenotypic profile with expression of epithelial, mesenchymal and neural markers. Cytogenetically, this tumor presents a unique abnormality – t(11;22)(p13;q12). Case: A 29-year-old male without significant medical history was admitted to our institution with gastrointestinal symptomatology. The physical examination and medical imaging studies revealed an extensive soft tissue mass filling the entire peritoneal cavity/pelvis. A fine-needle aspiration (FNA) of the abdominal mass was performed. The FNA smears revealed fragments of collagenous desmoplastic stroma and clusters of loosely cohesive small round cells that showed positivity for epithelial and myogenic markers. Cytogenetic analysis demonstrated rearrangement of the genes EWSR1 and WT1, resulting from the t(11;22)(p13;q12). Conclusion: DSRCT is an uncommon neoplasm that shares clinical and cytomorphologic features with other small round cell tumors. Therefore, a primary definitive diagnosis based on cytology specimens may be difficult but plausible and can be aided by a typical clinical presentation and ancillary immunocytochemical/cytogenetic studies.

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Occult Lymph Node Metastasis from Desmoplastic Small Round Cell Tumor Diagnosed by Fine Needle Aspiration Cytology

Cited by 14 publications

References 12 publications

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

Fine Needle Aspiration Biopsy with Adjunct Immunohistochemistry in Intraocular Tumor Management

Desmoplastic Small Round Cell Tumor: Diagnosis by Fine-Needle Aspiration Cytology

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