Occupational asthma due to spores of <i>Pleurotus ostreatus</i>

Vereda, Andrea; Quirce, Santiago; Fernández‐Nieto, Mar; Bartolomé, B.; Sastre, Joaquı́n

doi:10.1111/j.1398-9995.2006.01286.x

Cited by 13 publications

(5 citation statements)

References 6 publications

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“…The problems, especially allergies, caused by spores from fruiting bodies of cultivated mushrooms have been a medical and agricultural issue for nearly half a century (2,4,19). Historically, mushrooms with the sporeless trait were considered useful breeding material for eliminating these sporecaused problems.…”

Section: Resultsmentioning

confidence: 99%

“…Pegler (3) and Pleurotus ostreatus (Jacq.) P. Kumm (4). The large number of spores released by the cultivars also gives rise to other problems, such as damage to cultivation facilities, spread of mushroom disease (5), reduced commercial value (due to spores deposited on the mushrooms), and depletion of genetic diversity in the natural population of the mushroom species that are cultivated (6,7).…”

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confidence: 99%

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An MSH4 Homolog, stpp1 , from Pleurotus pulmonarius Is a “Silver Bullet” for Resolving Problems Caused by Spores in Cultivated Mushrooms

Okuda

Murakami

Honda

et al. 2013

Appl Environ Microbiol

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The enormous number of spores produced by fruiting bodies during cultivation of mushrooms can lead to allergic reactions of workers, reduction of commercial value, spread of mushroom disease, pollution of facilities, and depletion of genetic diversity in natural populations. A cultivar harboring a sporulation-deficient (sporeless) mutation would be very useful for preventing these problems, but sporeless commercial cultivars are very limited in usefulness because sporeless traits are often linked with traits that are unfavorable for commercial cultivation. Thus, identifying a causal gene of a sporeless phenotype not linked to the adverse traits in breeding and cultivation is crucial for the establishment of sporeless breeding using a strategy employing targeting induced local lesions in genomes (TILLING) in cultivated mushrooms. We used a Pleurotus pulmonarius (Fr.) Quél. sporeless strain to identify and characterize the single recessive gene controlling the mutation. The 3,853-bp stpp1 gene encodes a protein of 854 amino acids and belongs to the MutS homolog (MSH) family associated with mismatch repair in DNA synthesis or recombination in meiosis. Gene expression analysis of the fruiting body showed that this gene is strongly expressed in the gills. Phenotypic analysis of disruptants formed by gene targeting suggested a reproducible sporeless phenotype. Mutants deficient in a functional copy of this gene have no unfavorable traits for sporeless cultivar breeding, so this gene will be an extremely useful target for efficient and versatile sporeless breeding in P. pulmonarius and various other cultivated mushrooms.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

An MSH4 Homolog, stpp1 , from Pleurotus pulmonarius Is a “Silver Bullet” for Resolving Problems Caused by Spores in Cultivated Mushrooms

Okuda

Murakami

Honda

et al. 2013

Appl Environ Microbiol

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show abstract

“…Similar to the airway inflammation found after common allergen inhalation challenges, 18 an increase in sputum eosinophils has been observed after positive SICs to a number of occupational agents of high-molecular-weight [19][20][21][22][23][24] or low-molecular-weight [25][26][27][28][29][30][31][32] agents. The factors that influence the type of airway inflammation induced by the exposure to occupational agents are unclear.…”

Section: Induced Sputum In the Investigation Of Oamentioning

confidence: 79%

Advanced Diagnostic Studies

Lemière¹

2014

Journal of Occupational &Amp; Environmental Medicine

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“…Although sporelessness can hinder the reproduction and propogation of fungi, as every coin with two sides, the sporeless trait can be of benefit to mushroom cultivation because massive production of spores can cause many serious problems, including respiratory allergic reactions to workers [ 27 , 28 ] and mushroom production losses. However, sporelessness often coincides with poor quality and yield in mushrooms [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Meiosis in an asymmetric dikaryotic genome of Tremella fuciformis Tr01 facilitates new chromosome formation

Deng,

Guo,

Lin

et al. 2023

Genome Biol

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Background The dikaryotic stage dominates most of the life cycle in basidiomycetes, and each cell carries two different haploid nuclei. Accurate phasing of these two nuclear genomes and their interactions have long been of interest. Results We combine PacBio HiFi reads, Nanopore ultra-long reads, and Hi-C data to generate a complete, high-quality asymmetric dikaryotic genome of Tremella fuciformis Tr01, including Haplotypes A and B genomes. We assemble a meiotic haploid DBZ04 genome and detect three recombination events in these two haplotypes. We identify several chromosomal rearrangements that lead to differences in chromosome number, length, content, and sequence arrangement between these two haplotypes. Each nucleus contains a two-speed genome, harboring three accessory chromosomes and two accessory compartments that affect horizontal chromatin transfer between nuclei. We find few basidiospores are ejected from fruiting bodies of Tr01. Most monospore isolates sequenced belong to Tr01-Haplotype A genome architecture. More than one-third of monospore isolates carry one or two extra chromosomes including Chr12B and two new chromosomes ChrN1 and ChrN2. We hypothesize that homologous regions of seven sister chromatids pair into a large complex during meiosis, followed by inter-chromosomal recombination at physical contact sites and formation of new chromosomes. Conclusion We assemble two haplotype genomes of T. fuciformis Tr01 and provide the first overview of basidiomycetous genomes with discrete genomic architecture. Meiotic activities of asymmetric dikaryotic genomes result in formation of new chromosomes, aneuploidy of some daughter cells, and inviability of most other daughter cells. We propose a new approach for breeding of sporeless mushroom.

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Occupational asthma due to spores of Pleurotus ostreatus

Cited by 13 publications

References 6 publications

An MSH4 Homolog, stpp1 , from Pleurotus pulmonarius Is a “Silver Bullet” for Resolving Problems Caused by Spores in Cultivated Mushrooms

An MSH4 Homolog, stpp1 , from Pleurotus pulmonarius Is a “Silver Bullet” for Resolving Problems Caused by Spores in Cultivated Mushrooms

Advanced Diagnostic Studies

Meiosis in an asymmetric dikaryotic genome of Tremella fuciformis Tr01 facilitates new chromosome formation

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