Aim: This study determined the level of health-related quality of life (HRQoL) of migrant seasonal farmworkers and related factors and compared the levels of the HRQoL of migrant seasonal farmworkers (MSF), seasonal farmworkers (SF) and non-farmworkers (NF). Method: A two staged study was conducted. In the first step we determined the HRQoL scores of MSF and the related factors in the agricultural fields. In the second step, comparisons were made between the HRQoL scores of MSF and SF and NF. There were 100 participants in each group. In order to collect data, a socio-demographic questionnaire, General Health Questionnaire and WHOQOL BREF TR quality of life scale were used. Results: The socio-demographic attributes of MSF were as follows: 58.0% were female, 72% were married, 60.0% were uneducated, and 90.0% had income below the minimum wage. Conclusion: MSF scored lower than both other groups on HRQL for physical, psychological and national environmental domains. A life without basic comforts and challenging working conditions with physical and psychological problems negatively affects the quality of life of migrant seasonal farmworkers.