Occupational exposure to mercury vapour on genotoxicity and DNA repair

Cebulska-Wasilewska, Antonina; Panek, Agnieszka; Zabiński, Zbigniew; Moszczyński, P; Au, William W.

doi:10.1016/j.mrgentox.2005.06.009

Cited by 81 publications

(51 citation statements)

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“…Since after inhalation of elemental mercury vapour in the blood elemental mercury is oxidised to mercuric mercury (ATSDR, 1999) the following section summarises genotoxicity in human lymphocytes after exposure towards elemental mercury. In human lymphocytes genetic damage (in terms of chromosome aberrations) has been observed after occupational exposure to elemental and organic mercury (Verschaeve et al, 1976;Popescu et al, 1979;Cebulska-Wasilewska et al, 2005); sister chromatid exchanges (Popescu et al, 1979;Cebulska-Wasilewska et al, 2005) and DNA damage as measured by the alkaline version of the Comet assay (Cebulska-Wasilewska et al, 2005) were not statistically significantly increased in these studies. Repair efficiencies in lymphocytes of 25 workers exposed to elemental mercury vapour were reduced compared with 50 individuals nonoccupationally exposed, as measured by the X-rays challenge assay (Cebulska-Wasilewska et al, 2005).…”

Section: Genotoxicitymentioning

confidence: 68%

“…In human lymphocytes genetic damage (in terms of chromosome aberrations) has been observed after occupational exposure to elemental and organic mercury (Verschaeve et al, 1976;Popescu et al, 1979;Cebulska-Wasilewska et al, 2005); sister chromatid exchanges (Popescu et al, 1979;Cebulska-Wasilewska et al, 2005) and DNA damage as measured by the alkaline version of the Comet assay (Cebulska-Wasilewska et al, 2005) were not statistically significantly increased in these studies. Repair efficiencies in lymphocytes of 25 workers exposed to elemental mercury vapour were reduced compared with 50 individuals nonoccupationally exposed, as measured by the X-rays challenge assay (Cebulska-Wasilewska et al, 2005). In another study increased urinary 8-hydroxy-2-deoxyguanosine levels were observed in occupationally mercury-exposed persons (35 workers, 13 non-occupationally exposed individuals); urinary 8-hydroxy-2-deoxyguanosine levels correlated with both serum and urinary mercury concentration (Chen et al, 2005).…”

Section: Genotoxicitymentioning

confidence: 68%

“…As underlying mechanisms oxidative stress, disruption of microtubules as well as interactions with DNA damage response and DNA repair pathways are discussed (Christie et al, 1986;Cebulska-Wasilewska et al, 2005). Using isolated DNA, mercuric and especially methylmercuric chloride have been shown to bind covalently to endocyclic and exocyclic nitrogen sites of DNA bases (Li et al, 2006).…”

Section: Genotoxicitymentioning

confidence: 99%

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Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

2012

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EFSA was asked by the European Commission to consider new developments regarding inorganic mercury and methylmercury toxicity and evaluate whether the Joint FAO/WHO Expert Committee on Food Additives (JECFA) provisional tolerable weekly intakes for methylmercury of 1.6 µg/kg body weight (b.w.) and of 4 µg/kg b.w. for inorganic mercury were still appropriate. In line with JECFA, the CONTAM Panel established a tolerable weekly intake (TWI) for inorganic mercury of 4 µg/kg b.w., expressed as mercury. For methylmercury, new developments in epidemiological studies from the Seychelles Child Developmental Study Nutrition Cohort have indicated that n-3 long-chain polyunsaturated fatty acids in fish may counteract negative effects from methylmercury exposure. Together with the information that beneficial nutrients in fish may have confounded previous adverse outcomes in child cohort studies from the Faroe Islands, the Panel established a TWI for methylmercury of 1.3 µg/kg b.w., expressed as mercury. The mean dietary exposure across age groups does not exceed the TWI for methylmercury, with the exception of toddlers and other children in some surveys. The 95 th percentile dietary exposure is close to or above the TWI for all age groups. High fish consumers, which might include pregnant women, may exceed the TWI by up to approximately six-fold. Unborn children constitute the most vulnerable group. Biomonitoring data from blood and hair indicate that methylmercury exposure is generally below the TWI in Europe, but higher levels are also observed. Exposure to methylmercury above the TWI is of concern. If measures to reduce methylmercury exposure are considered, the potential beneficial effects of fish consumption should also be taken into account. Dietary inorganic mercury exposure in Europe does not exceed the TWI, but inhalation exposure of elemental mercury from dental amalgam is likely to increase the internal inorganic mercury exposure; thus the TWI might be exceeded. This opinion focuses only on the risks related to dietary inorganic mercury and methylmercury exposure and does not assess the nutritional benefits linked to certain foods (e.g. fish and other seafood).A call for annual collection of chemical contaminant occurrence data in food and feed, including mercury, was issued by EFSA in December 2010. In response, EFSA received 59 820 results on mercury in food from 20 European countries, mainly covering the period from 2004 to 2011. A total number of 59 650 results were described with sufficient detail to be used in the statistical analysis of the respective food groups; 98.2 % of the samples were for total mercury, 1.8 % for methylmercury and three samples for inorganic mercury.All the 20 food groups available at the first level of FoodEx were covered in the current data collection. The food groups 'Fish and other seafood' and 'Meat and meat products' dominated the food product coverage with 36.8 % and 17.6 % respectively. These were followed by 'Grain and grain-based products' at 7.8 % and 'Vegetables an...

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Section: Genotoxicitymentioning

confidence: 68%

Section: Genotoxicitymentioning

confidence: 68%

Section: Genotoxicitymentioning

confidence: 99%

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Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

2012

EFS2

463

160

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“…Human lymphocytes were separated from blood using Histopaque separation medium (Sigma) following the manufacturer's procedure. The lymphocytes were frozen and thawed according to standard procedures [9].…”

Section: Methodsmentioning

confidence: 99%

“…Detailed applied protocol of comet assay can be found in [9][10][11]. About 1000 cells (in 30 µl) have been embedded in agarose (120 µl) on a microscope slide.…”

Section: Comet Assaymentioning

confidence: 99%

Applications of Comet Assay for the Evaluation of Genotoxicity and DNA Repair Efficiency in Nanomaterials Research

et al. 2018

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The single cell gel electrophoresis method, known as comet assay, is a rapid and sensitive technique for testing novel chemicals and nanoparticles for genotoxicity, monitoring environmental contamination with genotoxins and human biomonitoring. In our studies we check the applicability of this method for the evaluation of biocompatibility of modified (MWNF) and non-modified multi-walled carbon nanotubes (MWNT) as well as potential genotoxicity of mercury(II) nitrate. The obtained results enabled us to conclude that the presence of Hg(NO3q2 (p 0.001) and MWNT (p 0.04) cause a significantly higher level of DNA damage in comparison to functionalised nanomaterials MWNF. It was implied that for the three investigated agents only mercury significantly enhanced genotoxic effect of X-ray exposure (p 0.001) and inhibition of radio-induced DNA damage repair. On the contrary, the presence of MWNF have no influence on cellular repair efficiencies, while incubation with MWNT causes apoptosis and consequently results in lack of attached cells. In conclusion, our results confirmed the genotoxicity of mercury and non-modified carbon nanotubes as well as the biocompatibility of modified nanotubes. Additionally, we proved the usefulness of comet method for the evaluation of genotoxicity and DNA repair under the influence of different compounds and nanomaterials.

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Quecksilber und anorganische Quecksilberverbindungen [MAK Value Documentation in German language, 2011]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 51. Lieferung, Ausgabe 2011 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik und Metabolismus Aufnahme, Verteilung, Ausscheidung Metabolismus Erfahrungen beim Menschen Einmalige Exposition Wiederholte Exposition Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Tierexperimentelle Befunde und In‐vitro‐Untersuchungen Akute Toxizität Subakute, subchronische und chronische Toxizität Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Sonstige Wirkungen Bewertung

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Occupational exposure to mercury vapour on genotoxicity and DNA repair

Cited by 81 publications

References 32 publications

Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

Applications of Comet Assay for the Evaluation of Genotoxicity and DNA Repair Efficiency in Nanomaterials Research

Quecksilber und anorganische Quecksilberverbindungen [MAK Value Documentation in German language, 2011]

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