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Global warming and elevated ozone (O 3 ) levels are gradually gaining widespread attention, and exposure to which may cause many physiological changes associated with cardiovascular events such as hypertension, cardiomyocyte apoptosis, etc. In addition, ischemic heart disease (IHD) is the leading cause of death worldwide. However, the contributions of temperature and O 3 , independently or in combination, to IHD mortality are not well understood. This study employs a two-stage analytical protocol (generalized additive model followed by meta-analysis) to explore the respective associations of temperature and O 3 with IHD mortality, and determine their possible mediation and interaction effects. Our results suggest that increases of 10 μg/m 3 in O 3 and 1 °C in temperature at lag01 day are associated with increased IHD mortality risks of 0.789% and 0.686%, respectively. O 3 can mediate the relationship between temperature and IHD mortality, with a pooled estimate of 0.140%, while temperature can mediate the association between O 3 and IHD mortality, with a pooled estimate of 0.162%. The additive and multiplicative interaction effects of O 3 and temperature were significantly associated with IHD mortality. The study findings demonstrate that higher temperature and O 3 concentrations can increase human IHD mortality risk through interaction and mediation effects, providing a scientific basis for the synergistic management of temperature and O 3 or associated interventions.
Global warming and elevated ozone (O 3 ) levels are gradually gaining widespread attention, and exposure to which may cause many physiological changes associated with cardiovascular events such as hypertension, cardiomyocyte apoptosis, etc. In addition, ischemic heart disease (IHD) is the leading cause of death worldwide. However, the contributions of temperature and O 3 , independently or in combination, to IHD mortality are not well understood. This study employs a two-stage analytical protocol (generalized additive model followed by meta-analysis) to explore the respective associations of temperature and O 3 with IHD mortality, and determine their possible mediation and interaction effects. Our results suggest that increases of 10 μg/m 3 in O 3 and 1 °C in temperature at lag01 day are associated with increased IHD mortality risks of 0.789% and 0.686%, respectively. O 3 can mediate the relationship between temperature and IHD mortality, with a pooled estimate of 0.140%, while temperature can mediate the association between O 3 and IHD mortality, with a pooled estimate of 0.162%. The additive and multiplicative interaction effects of O 3 and temperature were significantly associated with IHD mortality. The study findings demonstrate that higher temperature and O 3 concentrations can increase human IHD mortality risk through interaction and mediation effects, providing a scientific basis for the synergistic management of temperature and O 3 or associated interventions.
Background/Aim: The impact of exercise on pediatric tumor biology is essentially unknown. We investigated the effects of regular exercise on tumor proteome profile (as assessed with liquid chromatography with tandem mass spectrometry) in a mouse model of one of the most aggressive childhood malignancies, high-risk neuroblastoma (HR-NB). Methods: Tumor samples of 14 male mice (aged 6-8 weeks) that were randomly allocated into an exercise (5-week combined aerobic and resistance training) or nonexercise control group (6 and 8 mice per group, respectively) were analyzed. The Search Tool for the Retrieval of Interacting Genes/Proteins database was used to generate a protein-protein interaction (PPI) network and enrichment analyses. The Systems Biology Triangle (SBT) algorithm was applied for analyses at the functional category level. Results: Tumors of exercised mice showed a higher and lower abundance of 101 and 150 proteins, respectively, compared to controls [false discovery rate (FDR)<0.05], which were enriched in metabolic pathways, aminoacid metabolism, regulation of hormone levels, and peroxisome proliferator-activated receptor signaling pathway (FDR<0.05). The SBT algorithm indicated that 184 and 126 categories showed a lower and higher abundance, respectively, in the tumors of exercised mice (FDR<0.01). Categories with lower abundance were involved in energy production while those with higher abundance were related to transcription/translation, apoptosis, and tumor suppression. Conclusion: Regular exercise altered the abundance of hundreds of intratumoral proteins and molecular pathways, particularly those involved in energy metabolism, apoptosis, and tumor suppression. These findings provide preliminary evidence of the molecular mechanisms underlying potential effects of exercise in HR-NB.
Background eHealth literacy is critical for evaluating abilities in locating, accessing, and applying digital health information to enhance one’s understanding, skills, and attitudes toward a healthy lifestyle. Prior research indicates that enhancing eHealth literacy can improve health behaviors such as physical activity (PA). Physical literacy (PL) refers to the ability to develop sustainable PA habits, taking into account various aspects of an individual. Notably, university students have shown a decline in PA and possess low PL levels. However, the connection between eHealth literacy and PL in this demographic has not been extensively studied, and it remains uncertain whether PA acts as a mediator between eHealth literacy and PL. Objective This study examines the extent to which PA mediates the link between eHealth literacy and PL in Chinese university students and explores gender differences in these variables. Methods In February 2022, a cross-sectional survey was administered to 1210 students across 3 universities in China. The instruments used were the Perceived PL Instrument, the International Physical Activity Questionnaire, and the Chinese version of the eHealth Literacy Scale. Correlations between eHealth literacy, PA, and PL were analyzed using Pearson product-moment correlation and multiple linear regression, while mediation models helped elucidate the interactions among the 3 variables. Results The response rate for the study was 92.9% (1124/1210). In the mediation analysis, eHealth literacy showed a significant direct effect on PL, with a coefficient of 0.78 (β .75, SE 0.02; P<.001). Moderate to vigorous physical activity (MVPA) accounted for 2.16% of the total effect, suggesting that MVPA partially mediates the relationship between eHealth literacy and PL. Additionally, male students outperformed female students in terms of MVPA (t636=4.94; P<.001) and PL (t636=3.18; P<.001), but no significant differences were found in eHealth literacy (t636=1.23; P=.22). Conclusions The findings indicate that MVPA serves as a mediator in the link between eHealth literacy and PL among university students. Students with low eHealth literacy or limited PA are less likely to be physically literate. Thus, eHealth literacy plays a crucial role in enhancing PL and PA, especially when interventions targeting PL are implemented. Our results also suggest a need for targeted health education interventions aimed at improving MVPA and PL among female students, while also recognizing that eHealth literacy is comparable across genders at universities.
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