The current study was aimed to investigate the comparison of occupational stress and clinical performance among nurses of public and private sector: role of emotional intelligence. The survey method was used as a method of data collection. The population of the study was employed nurses in Nishter hospital, Combined Military, Children Complex Hospital, City Hospital Multan, Buch International Hospital Multan. Purposive sampling technique was utilized to select the 100 (public, n = 50, private, n = 50). Results reveal that occupational stress is negatively correlated with clinical performance. Occupational stress has a negative impact on clinical performance among nurses. Emotional intelligence significantly mediates the relationship between occupational stress and clinical performance among nurses. Moreover, Occupational stress, emotional intelligence, and clinical performance were found to have significant variations concerning the sector of nurses. Nurses of private sector showed the greater level of occupational stress as compared to public sector. In addition, clinical performance of public sector nurses was reported higher as compared to private sector. Developing emotional intelligence and providing support and opportunities are crucial strategies to reduce occupational stress among nurses.