Occurrence and abundance of anisakid nematode larvae in five species of fish from southern Australian waters

Shamsi, Shokoofeh; Eisenbarth, Albert; Saptarshi, Shruti R.; Beveridge, Ian; Gasser, Robin B.; Lopata, Andreas L.

doi:10.1007/s00436-010-2134-1

Cited by 46 publications

(27 citation statements)

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“…Although little is known about the ecology and epidemiology of anisakid nematodes in Australia [16,17,[29][30][31][32][33], a recent preliminary epidemiological survey of five commercially significant species of fish in southern Australian waters showed that four were infected with at least one of five anisakids (Anisakis, Contracaecum types I and II and Hysterothylacium types IV and VIII) [16]. In another report, Shamsi [34] described eight new types of Hysterothylacium from 20 fish species (n 5 81) collected from different parts of Australia.…”

Section: Introductionmentioning

confidence: 99%

“…Using suitable genetic markers, electrophoretic and sequencing methods can overcome this limitation [15]. A number of studies have demonstrated that the first and second internal transcribed spacers (ITS-1 and ITS-2, respectively) of nuclear ribosomal DNA (rDNA) provide suitable genetic markers for the identification of anisakid species, irrespective of developmental stage [16][17][18][19][20][21][22][23][24][25][26][27], and PCR-coupled mutation scanning of the ITS-1 and/or ITS-2 combined with targeted sequencing [28] provides a powerful approach for exploring the genetic composition of anisakid populations and for investigating their biology.…”

Section: Introductionmentioning

confidence: 99%

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Mutation scanning‐based analysis of anisakid larvae from Sillago flindersi from Bass Strait, Australia

et al. 2012

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Anisakidosis is an important fish-borne disease caused by the larvae of anisakid nematodes, which affects humans and a range of other animals. The accurate identification of members of this nematode group is central to investigating the epidemiology of the parasites and in the surveillance and control of anisakidosis. It is now well known that morphological identification alone does not allow specific identification, particularly of larval stages. To better understand the epidemiology of anisakid nematodes in southern Australian fishes and the potential risks posed to human health, a survey of 50 specimens of the commercially important fish, Sillago flindersi, from Bass Strait, Australia was conducted. We characterised anisakid larvae by PCR-coupled mutation scanning, sequencing and phylogenetic analyses of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA. This study revealed that 92% of the S. flindersi examined were infected with anisakids (n=194), which were represented by seven genotypes. Phylogenetic analyses of the genotypes defined herein, together with reference sequence for Anisakis pegreffii and Hysterothylacium sp. from public databases (i.e. GenBank), revealed the presence of A. pegreffii (n=24), Hysterothylacium larval type IV (n=90) and Hysterothylacium larval type VIII (n=80) in S. flindersi. Thus, the PCR-coupled mutation scanning approach employed herein is an effective tool for the genetic characterisation of anisakid nematodes for diagnostic and analytical purposes (nucleotide sequences reported in this paper are available in the GenBank database under accession nos. JN631796-809).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mutation scanning‐based analysis of anisakid larvae from Sillago flindersi from Bass Strait, Australia

et al. 2012

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“…The presence of larval stages of Anisakid nematodes in fish is a problem for commercial fishing industries due to the potential risk of transmission of zoonotic diseases such as anisakiasis (6)(7)(8), and foodborne allergenicity (9,10). Currently, fish diseases, in particular those caused by parasites, constitute one of the most important problems and challenges confronting fish farming (11).…”

Section: Introductionmentioning

confidence: 99%

Parasites and hepatic histopathological lesions in lisa (Mugil incilis) from Totumo mash, North of Colombia

Olivero

2013

Rev MVZ Córdoba

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Objective. To assess the levels of parasitism by nematodes and trematodes, as well as the hepatic histopathological alterations present in Mugil incilis (Lisa) from Totumo marsh, North of Colombia. Materials and methods. Between July 2004 and June 2005, 500 fish were collected at Totumo Marsh (75°16'W and 10°44´N), North of Colombia. Morphometric and parasitic parameters were determined for each specimen, and the hepatic histopathological status of the liver was assessed by analyzing liver slides stained with hematoxylin and eosin. Results. Nematode larvae isolated from Mugil incilis corresponded to Contracaecum spp. Parasite prevalence was 60.49%. Parasitic mean abundance and mean intensity were 4.8±1.05 and 7.02±1.49, nematodes per fish, respectively. The correlation between nematode mean abundance and fish length was significant and positive (r=0.525, p<0.0001), but negative for condition factor (r=-0.109, p=0.014). Hepatic histopathological analysis revealed the presence of encapsulated trematode larvae as the main finding. However, the presence of inflammation, granulomas, steatosis and necrosis, were also registered as secondary alterations. Conclusions. Lisas collected at Totumo Marsh are parasitized with nematodes and trematodes. These fish have different histopathological lesions in the liver tissue, being the most important the presence of trematode encapsulated cyst that generate inflammatory reactions, and negatively correlate with morphometric markers of fish health.Key words: Anisakiasis, fishes, nematode, trematode, zoonoses (Source: CAB). RESUMENObjetivo. Evaluar los niveles de parasitismo por nemátodos y tremátodos, así como las alteraciones histopatológicas hepáticas presentes en Mugil incilis (Lisa) de la Ciénaga del Totumo, Norte de Colombia. Materiales y métodos. Entre Julio de 2004 y Junio de 2005, 500 peces fueron recolectados en la ciénaga del Totumo (75°16'W y 10°44´N), norte de Colombia. Índices morfométricos y parasitarios fueron determinados para cada espécimen, y el estatus histopatológico hepático fue evaluado analizando cortes de hígado teñidos con hematoxilina y eosina. Resultados. Los nemátodos aisladosRev.MVZ Córdoba 18(1):3288-3294, 2013. ORIGINAL 3289de Mugil incilis correspondieron a Contracaecum spp. La prevalencia del parásito fue 60.49%. La abundancia media y la intensidad media parasitaria fueron 4.8±1.05 y 7.02±1.49, larvas por pez, respectivamente. La correlación entre la abundancia media de nemátodos y la longitud de las lisas fue significativa y positiva (r=0.525, p<0.0001), pero negativa para el factor de condición (r=-0.109, p=0.014). El análisis de la histopatología hepática reveló la presencia de larvas encapsuladas de tremátodos, como el hallazgo principal. Sin embargo, la presencia de inflamación, granulomas, esteatosis y necrosis, también fueron registradas como alteraciones secundarias. Conclusiones. Las Lisas colectadas en la ciénaga del Totumo están parasitadas con nemátodos y tremátodos. Estos peces poseen diversas lesiones histopatológicas en el te...

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“…Although larval stages are found commonly in species of teleosts, frequently in species used for human consumption, identification beyond the level of genus is virtually impossible using morphological methods, resulting in the larvae being assigned to various morphotypes designated by Roman numerals (Cannon 1977a(Cannon , 1977bCannon 1989, 1990;Bruce 1990aBruce , 1990bNash 1998;Lymbery et al 2002;Doupé et al 2003;Muñoz et al 2007;Shamsi 2007;Shamsi et al 2008Shamsi et al , 2011aShamsi et al , 2011bShamsi et al , 2012Jabbar et al 2012aJabbar et al , 2012b. The more recent introduction of molecular techniques has allowed the identification of some larval stages in teleosts and, as a consequence, the identification of life-cycle patterns Nascetti 2006, 2008).…”

Section: Introductionmentioning

confidence: 99%

Larval anisakid nematodes in teleost fishes from Lizard Island, northern Great Barrier Reef, Australia

Jabbar

Asnoussi

Norbury

et al. 2012

Mar. Freshwater Res.

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A survey was undertaken to characterise larval anisakid nematodes present in teleosts at Lizard Island on the northern Great Barrier Reef. In total, 464 fish were examined from 32 families, 62 genera and 107 species. Anisakid nematodes were found in 46 (9.9%) of the fish examined. Infections in Atherinidae, Lethrinidae, Lutjanidae and Serranidae were moderately prevalent, with the intensities of infection ranging from 1 to 80; whereas in the Sphyraenidae and Scombridae, the prevalence of infection was very high, with intensities ranging from 1 to .375 anisakids. A combined morphological and molecular-phylogenetic approach was employed to identify larval anisakid nematodes to species and/ or genotypes. The nematodes examined were identified as Anisakis typica (three genotypes based on molecular characterisation), Terranova Types I (five genotypes) and II (five genotypes) and Hysterothylacium Types IV, V (four genotypes), VI and X. The findings of the present study provide some insights into the distribution of larval anisakid nematodes in coral-reef fishes and a basis for future investigations of anisakid populations in marine fishes.Additional keywords: Anisakidae, first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA, Nematoda, single-strand conformation polymorphism (SSCP) analysis.

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Occurrence and abundance of anisakid nematode larvae in five species of fish from southern Australian waters

Cited by 46 publications

References 29 publications

Mutation scanning‐based analysis of anisakid larvae from Sillago flindersi from Bass Strait, Australia

Mutation scanning‐based analysis of anisakid larvae from Sillago flindersi from Bass Strait, Australia

Parasites and hepatic histopathological lesions in lisa (Mugil incilis) from Totumo mash, North of Colombia

Larval anisakid nematodes in teleost fishes from Lizard Island, northern Great Barrier Reef, Australia

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