Occurrence and Antibiotic Sensitivity of Escherichia coli and Salmonella spp. in Retail Chicken Meat at Selected Markets in Valencia City, Bukidnon, Philippines

Abstract

Aim: Contaminated retail chicken meat has been implicated with food poisoning caused by Escherichia coli and Salmonella spp. The present study was aimed to determine the occurrence and antibiotic sensitivity of E. coli and Salmonella spp. in retail chicken meat at selected markets in Valencia City, Bukidnon, Philippines. Methods: A total of 50 retailed chicken meats which comprised of drumsticks (n=25) and wings (n=25) were collected aseptically from three selected markets (2 supermarkets and 1 wet market) in Valencia City, Bukidnon, Philippines and examined for the occurrence of Escherichia coli and Salmonella spp. Identification of bacterial isolates was through morphological characteristics, Gram-staining, and biochemical tests or growth-dependent identification methods. The antibiotic sensitivity of the pure isolates to amoxicillin (25 μg), ampicillin (10 μg), chloramphenicol (30 μg), and streptomycin (10 μg) was determined through a standard antimicrobial disk diffusion test. Results: Results revealed that 68% (17/25) of the drumsticks and 96% (24/25) of the wings samples were contaminated with E. coli while 4% (1/25) of the drumsticks and 32% (8/25) of wings samples were contaminated with Salmonella spp. In total, 41 pure isolates of E. coli and 9 pure isolates of Salmonella spp. were obtained. Antimicrobial disk diffusion test revealed that 87.80% (36/41) of E. coli isolates and 100% (9/9) Salmonella spp. isolates are resistant to amoxicillin, and 36.58% (15/41) of E. coli isolates and 88.88% (8/9) Salmonella spp. isolates are resistant to ampicillin. Moreover, 29.27% (12/41) of E. coli isolates and 88.88% (8/9) of Salmonella spp. isolates are resistant to both amoxicillin and ampicillin. Majority (96%) of isolates are sensitive to streptomycin. Conclusion: There was a high occurrence of E. coli contamination in retail chicken meat in Valencia City, Bukidnon, Philippines. E. coli and Salmonella spp. from these retail meat showed high resistance to amoxicillin and ampicillin..

Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines

“…In the present study, 54.6% of the samples were contaminated with E. coli , with the highest prevalence being observed in chicken meat (70.6–82.7%). The high-level contamination of E. coli in chicken was consistent with that reported by previous studies conducted in the Philippines [ 15 , 22 ], China [ 23 ], and Bangladesh [ 24 ]. Contamination of meat samples with pathogenic E. coli usually indicates poor hygiene during slaughter and handling, and unsuitable storage after slaughter [ 22 , 23 ].…”

“…The high-level contamination of E. coli in chicken was consistent with that reported by previous studies conducted in the Philippines [ 15 , 22 ], China [ 23 ], and Bangladesh [ 24 ]. Contamination of meat samples with pathogenic E. coli usually indicates poor hygiene during slaughter and handling, and unsuitable storage after slaughter [ 22 , 23 ]. Another source of contamination is meat supplied from unauthorized abattoirs (e.g., no proper hygiene inspections by authorities), which is then sold at local retail meat shops.…”

Antimicrobial resistance profiles in Escherichia coli isolated from whole-chicken carcasses from conventional, antibiotic-free, and organic rearing systems

“…Studies carried out with E. coli isolated from the poultry production chain have demonstrated variable frequencies of resistance to antimicrobials. However, ampicillin, tetracycline, and nalidixic acid have often been reported to have the highest resistance rates (Elumba et al, 2018;Abraham et al, 2019;Varga et al, 2019;Jahantigh et al, 2020;Musa et al, 2020;Pesciaroli et al, 2020;Rabello et al, 2020). In addition, this study Semina: Ciênc.…”

Use of veterinary medicinal products in the Philippines: regulations, impact, challenges, and recommendations

“…In Valencia City, Bukidnon, the Philippines, isolates of E. coli and Salmonella spp. Collected from retail chicken meat at selected markets were resistant to amoxicillin and ampicillin [ 16 ]. Legario et al [ 17 ] reported recovery of Streptococci from Nile tilapia farmed in the Philippines, exhibiting resistance to oxolinic acid and sulphamethoxazole–trimethoprim.…”