Occurrence and antimicrobial resistance of <i>Salmonella</i> isolated from retail meats in Anhui, China

Wu, Wei; Chen, Jing; Shao, Xiaoqiu; Huang, Pan; Zhang, Jing; Ye, Yingwang

doi:10.1002/fsn3.2266

Cited by 23 publications

(18 citation statements)

References 74 publications

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“…Owing to the excessive use of antimicrobial agents in animal husbandry over the last few decades and the horizontal spread of resistance genes, AMR in Salmonella has become a major concern ( Foley et al, 2008 ). All isolates identified in this study were MDR and were broadly resistant to conventional antimicrobials, suggesting that most bacteria have acquired resistance traits under prolonged selection pressures, which is consistent with many previous findings ( Yang et al, 2020 ; Sun et al, 2021 ; Wang et al, 2021 ). We also found variations in the resistance patterns of isolates from different periods, with the isolates obtained in the first collection period exhibiting a marked increase in resistance to EFT and newer-generation antimicrobials as well as an increase in susceptibility to TET.…”

Section: Discussionsupporting

confidence: 91%

“…Frontiers in Microbiology 08 frontiersin.org (Yang et al, 2020;Sun et al, 2021;Wang et al, 2021). We also found variations in the resistance patterns of isolates from different periods, with the isolates obtained in the first collection period exhibiting a marked increase in resistance to EFT and newer-generation antimicrobials as well as an increase in susceptibility to TET.…”

Section: Discussionmentioning

confidence: 60%

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Phenotypic and genotypic characterization of antimicrobial resistance profiles in Salmonella isolated from waterfowl in 2002–2005 and 2018–2020 in Sichuan, China

Guan

Li²,

Li³

et al. 2022

Front. Microbiol.

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Salmonella enterica is a widespread foodborne pathogen with concerning antimicrobial resistance (AMR). Waterfowl are a major source of Salmonella transmission, but there are few systematic studies on Salmonella prevalence in waterfowl species. In this study, 126 Salmonella isolates (65 collected in 2018–2020 and 61 collected in 2002–2005) were obtained from waterfowl samples in Sichuan, China. Their serotypes, pulsed-field gel electrophoresis (PFGE) types, and phenotypic and genotypic AMR profiles were systematically examined. The isolates were distributed in 7 serotypes, including serovars Enteritidis (46.0%), Potsdam (27.8%), Montevideo (7.9%), Cerro (6.3%), Typhimurium (4.8%), Kottbus (4.0%) and Apeyeme (3.2%). Their PFGE characteristics were diverse; all isolates were distributed in four groups (cutoff value: 60.0%) and 20 clusters (cutoff value: 80.0%). Moreover, all isolates were multidrug resistant, and high rates of AMR to lincomycin (100.0%), rifampicin (100.0%), sulfadiazine (93.7%), erythromycin (89.7%), ciprofloxacin (81.0%), and gentamicin (75.4%) were observed. Finally, 49 isolates were subjected to whole-genome sequencing, and a wide variety of AMR genes were found, including multiple efflux pump genes and specific resistance genes. Interestingly, the tet(A)/tet(B) and catII resistance genes were detected in only isolates obtained in the first collection period, while the gyrA (S83F, D87N and D87G) and gyrB (E466D) mutations were detected at higher frequencies in the isolates obtained in the second collection period, supporting the findings that isolates from different periods exhibited different patterns of resistance to tetracycline, chloramphenicol and nalidixic acid. In addition, various incompatible plasmid replicon fragments were detected, including Col440I, Col440II, IncFIB, IncFII, IncX1, IncX9, IncI1-I and IncI2, which may contribute to the horizontal transmission of AMR genes and provide competitive advantages. In summary, we demonstrated that the Salmonella isolates prevalent in Sichuan waterfowl farms exhibited diverse serotypes, multiple AMR phenotypes and genotypes, and AMR changes over time, indicating their potential risks to public health.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 60%

Phenotypic and genotypic characterization of antimicrobial resistance profiles in Salmonella isolated from waterfowl in 2002–2005 and 2018–2020 in Sichuan, China

Guan

Li²,

Li³

et al. 2022

Front. Microbiol.

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“…Out of 26 papers selected, 15 papers were from China [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ] and the rest (11 papers) were from the USA [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. The data extracted contain 13,018 food samples, out of which 5000 samples were AMR positive (2276 and 2724 from China and the USA, respectively).…”

Section: Resultsmentioning

confidence: 99%

Systematic Surveillance and Meta-Analysis of Antimicrobial Resistance and Food Sources from China and the USA

et al. 2022

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Since the discovery of antibiotics in the 20th century, they have been used to fight against infections. The overuse of antibiotics in the wider environment has resulted in the emergence of multidrug-resistant bacteria. In developing countries such as China and developed countries such as the USA, there is evidence of the high pervasiveness of antibiotic-resistant infections. However, the studies on the spread of antibiotic-resistant microorganisms that inform about the consequences are limited. The aim of our study was to analyze and compare antimicrobial resistance (AMR) identified in published research papers from that found in different food sources, which were published between 2012 and December 2021, covering most retail food items. Out of 132 research papers identified, 26 papers have met our strict criteria and are included in the qualitative and quantitative analysis. The selected papers led to 13,018 food samples, out of which 5000 samples were contaminated, including 2276 and 2724 samples from China and the USA, respectively. Meat, aquatic products, milk, and eggs show high to medium potential for AMR exposure to Gram-positive bacteria such as Staphylococcus, Enterococci, etc. and Gram-negative foodborne pathogens such as Campylobacter, Salmonella, Vibrio, etc. Most of the food samples show antibiotic resistance to β-lactams, tetracycline, quinolones, and aminoglycosides. Retail food products such as meat, sea food, and some other food products, as well as AMR genetics and technically important bacteria, are proposed to be better merged with mitigation strategies and systematic One Health AMR surveillance to minimize the knowledge gaps and facilitate comprehensive AMR risk computation for the consumers.

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“…Contrary to the current study, in a previous report by Hai et al [ 30 ] from China, more than 90.0% of the Salmonella isolates harbored stn and sopB genes. Also, Wang et al [ 31 ] from China reported higher incidence rates for sopB (100.0%) and spvC (93.8%) genes among Salmonella serovars isolated from 120 retail meat samples, including chicken ( n = 45), duck ( n = 30), and pork ( n = 45). In the prior studies from Iran [ 6 ], Tunisia [ 23 ], and Nigeria [ 27 ], the prevalence rates of spvC gene were 50.8%, 45.8%, and 59.1%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of virulence factors and carbapenemase genes in Salmonella enterica serovar Enteritidis isolated from chicken meat and egg samples in Iraq

et al. 2022

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Background Food-borne infections mainly due to Salmonella enterica serovar Enteritidis (S. Enteritidis) are major concerns worldwide. S. Enteritidis isolates may serve as reservoirs for spreading antimicrobial drug resistance genes including carbapenemases. This study aimed to screen the occurrence of virulence factors, carbapenemases, and antibiotic resistance genes in S. Enteritidis isolated from chicken meat and eggs in Iraq. Results In total, 1000 non-duplicated chicken meat and 1000 egg samples were collected during 2019–2020. Presumptive S. Enteritidis isolates were initially identified by standard bacteriology tests and then were confirmed using polymerase chain reaction (PCR). Carbapenem resistance was detected using the disk diffusion method. Virulence and carbapenemase genes were screened using the PCR method. In total, 100 (5.0%) S. Enteritidis isolates were identified from 2000 samples collected using phenotypic and molecular methods. These isolates were identified from 4.9% chicken meat (n = 49/1000) and 5.1% egg (n = 51/1000) samples, respectively. The most and the least susceptibility was found to gentamicin and ceftazidime antibiotics, respectively. The prevalence of different virulence factors were as follows: phoP/Q (40.0%), traT (30.0%), stn (22.0%), slyA (11.0%), and sopB (9.0%). Among 20 carbapenem-resistant S. Enteritidis isolates, the most predominant carbapenemase gene was blaIMP (35.0%, n = 7), followed by blaOXA−48−like (25.0%, n = 5), and blaNDM (10.0%, n = 2), while the blaKPC and blaVIM genes were not detected. The coexistence of blaIMP, blaOXA−48−like, and blaNDM genes was determined in two isolates. The prevalence of different antibiotic resistance genes were as follows: tetA (87.1%), tetB (87.1%), dfrA1 (77.6%), and sul1 (83.6%). Conclusion Considering the existence of carbapenem-resistant S. Enteritidis harboring different virulence and antibiotic resistance genes in chicken meat and egg samples, adherence to proper hygienic conditions should be considered.

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Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Cited by 23 publications

References 74 publications

Phenotypic and genotypic characterization of antimicrobial resistance profiles in Salmonella isolated from waterfowl in 2002–2005 and 2018–2020 in Sichuan, China

Phenotypic and genotypic characterization of antimicrobial resistance profiles in Salmonella isolated from waterfowl in 2002–2005 and 2018–2020 in Sichuan, China

Systematic Surveillance and Meta-Analysis of Antimicrobial Resistance and Food Sources from China and the USA

Occurrence of virulence factors and carbapenemase genes in Salmonella enterica serovar Enteritidis isolated from chicken meat and egg samples in Iraq

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