Occurrence and bioacessibility of mycotoxins in fish feed

Nogueira, Wesclen Vilar; Oliveira, Francine Kerstner de; Sibaja, Karen Vanessa Marimón; Garcia, Sabrina de Oliveira; Kupski, Larine; Souza, Michele Moraes de; Tesser, Marcelo Borges; Garda‐Buffon, Jaqueline

doi:10.1080/19393210.2020.1766577

Cited by 24 publications

(17 citation statements)

References 28 publications

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“…Studies concerning the bioaccessibility of AFB1 are scarce. Similarly to the obtained results, AFB1 bioaccessibility was between 83-108% for peanut slurry [35], between 85 and 98%, for various spiked food matrices (peanut, pistachio, hazelnut, dried figs, paprika, wheat, and maize) [38]; and 85% in fish feed [39]. Conversely, lower values were obtained for AFB1 bioaccessibility in spiked loaf bread during the stomach and the duodenal digestion reaching 54% and 26%, respectively [17].…”

Section: Bread Typesupporting

confidence: 80%

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Escrivá

Agahi

Vila‐Donat

et al. 2021

Toxins

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The presence of mycotoxins in cereals and cereal products remains a significant issue. The use of natural ingredients such as pumpkin and whey, which contain bioactive compounds, could be a strategy to reduce the use of conventional chemical preservatives. The aim of the present work was to study the bioaccessibility of aflatoxin B1 (AFB1) and ochratoxin (OTA) in bread, as well as to evaluate the effect of milk whey (with and without lactic acid bacteria fermentation) and pumpkin on reducing mycotoxins bioaccessibility. Different bread typologies were prepared and subjected to an in vitro digestion model. Gastric and intestinal extracts were analyzed by HPLC–MS/qTOF and mycotoxins bioaccessibility was calculated. All the tested ingredients but one significantly reduced mycotoxin intestinal bioaccessibility. Pumpkin powder demonstrated to be the most effective ingredient showing significant reductions of AFB1 and OTA bioaccessibility up to 74% and 34%, respectively. Whey, fermented whey, and the combination of pumpkin-fermented whey showed intestinal bioaccessibility reductions between 57–68% for AFB1, and between 11–20% for OTA. These results pointed to pumpkin and milk whey as potential bioactive ingredients that may have promising applications in the bakery industry.

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Section: Bread Typesupporting

confidence: 80%

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Escrivá

Agahi

Vila‐Donat

et al. 2021

Toxins

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“…OTA, as a common pollutant in food and feed raw materials, widely exists in various food crops and their by‐products, which can cause damage to kidney, liver, immune organs, reproductive system, etc., and pose a serious threat to animal and human health, and bring huge economic losses to animal husbandry 17 . Previous studies showed that OTA also have toxicological effects to human and animals 18 . The results showed OTA induces apoptosis in HK‐2 through regulating lipid raft/PTEN/AKT signaling pathway.…”

Section: Discussionmentioning

confidence: 97%

Ochratoxin A induces human kidney tubular epithelial cell apoptosis through regulating lipid raft/PTEN/AKT signaling pathway

Song

Liu

Zhao

et al. 2021

Environmental Toxicology

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Ochratoxin A (OTA) is a fungal toxin that causes serious threat to human health. OTA could lead to the injury of various tissues, especially kidney injury. However, the toxic effects of OTA on human kidney tubular epithelial cell (HK‐2) and the possible mechanism remains poorly understood. This study was to investigate the toxic effects of OTA on HK‐2 and elucidate the molecular mechanism. HK‐2 cells were treated OTA to evaluate the effect of OTA on cell viability and apoptosis. OTA inhibited the growth of HK‐2 in a concentration‐dependent manner. With the concentration increased, OTA significantly lead to the apoptosis of HK‐2. OTA could increase the levels of reactive oxygen species (ROS) and Malondialdehyde (MDA). Superoxide dismutase (SOD) and glutathione (GSH) activities were decreased by OTA. Furthermore, OTA increased Caspase‐3 and Bax expression and decreased BCL2 expression. Compared to the control group, the expression of PTEN was increased and the expression of PI3K and AKT were decreased in OTA treated groups. In addition, we found OTA could disrupt the formation of lipid raft by attenuating sphingomyelin and cholesterol levels. In conclusion, our results indicated that OTA induces apoptosis in HK‐2 through regulating PTEN/AKT signaling pathway via disrupting lipid raft formation.

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“…Different studies have shown that some mycotoxins carry-over from feed into edible tissues or fluids from animals [ 72 , 73 , 74 ]. As far as the foodstuffs from aquaculture industries are concerned, the occurrence of aflatoxins, ochratoxin A, and Fusarium mycotoxins, such as thrichotecenes and the emerging fusariotoxins, have been reported in the literature [ 75 , 76 , 77 , 78 , 79 ]. Tolosa et al [ 76 ] determined the occurrence of enniatins (ENs) in feed for farmed fish and in fish samples of sea bass and sea bream.…”

Section: Discussionmentioning

confidence: 99%

Cytoprotective Effects of Fish Protein Hydrolysates against H2O2-Induced Oxidative Stress and Mycotoxins in Caco-2/TC7 Cells

et al. 2021

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Many studies report the potent antioxidant capacity for fish protein hydrolysates, including radical scavenging activity and inhibition ability on lipid peroxidation (LPO). In this study, the in vitro cytotoxicity of protein hydrolysates from different salmon, mackerel, and herring side streams fractions was evaluated in the concentration range from 1 to 1:32 dilution, using cloned human colon adenocarcinoma cells TC7 (Caco-2/TC7) by MTT and PT assays. The protein hydrolysates’ antioxidant capacity and oxidative stress effects were evaluated by LPO and reactive oxygen species (ROS) generation, respectively. The antioxidant capacity for pure and bioavailable hydrolysate fraction was also evaluated and compared. Additionally, mycotoxin levels were determined in the fish protein hydrolysates, and their cytoprotective effect against T-2 toxin was evaluated. Both hydrolysates and their bioavailable fraction induced similar cell viability rates. The highest cytoprotective effect was obtained for the salmon viscera protein hydrolysate (HSV), which increased the cell viability by 51.2%. ROS accumulation induced by H2O2 and LPO was suppressed by all pure hydrolysates. The cytoprotective effect of hydrolysates was observed against T-2. Moreover, the different fish fraction protein hydrolysates contain variable nutrients and unique bioactive peptide composition showing variable bioactivity, which could be a useful tool in developing dietary supplements with different target functional properties.

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Occurrence and bioacessibility of mycotoxins in fish feed

Cited by 24 publications

References 28 publications

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Bioaccessibility Study of Aflatoxin B1 and Ochratoxin A in Bread Enriched with Fermented Milk Whey and/or Pumpkin

Ochratoxin A induces human kidney tubular epithelial cell apoptosis through regulating lipid raft/PTEN/AKT signaling pathway

Cytoprotective Effects of Fish Protein Hydrolysates against H2O2-Induced Oxidative Stress and Mycotoxins in Caco-2/TC7 Cells

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