Occurrence and characteristics of staphylococci and enterococci in retail fish used for human consumption in Turkey

Külahci, Meryem Burcu; Gündoğan, N.

doi:10.31590/ejosat.1012061

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…In this study, bacteriological examination of 80 fish samples revealed the occurrence of Enterococcus species in 42 (52.5%) of the examined fish samples, where E. faecalis and E. faecium were the most identified species. A higher result was obtained by Mendoza et al [23] [25]. As well, much lower percentages of 4% and 2.8% were recorded by Adamu et al [26] and El-Kader Mousa-Balabel [27] from fresh water fish in Nigeria (Clarias gariepinus, Labeo senegalensis, and Clarias angularis) and Egypt (O. niloticus), respectively.…”

Section: Disscussionmentioning

confidence: 75%

Prevalence, Antimicrobial Susceptibility, and Virulence Gene Profile of Enterococcus Species isolated from Some Farmed Fish Retailed in Zagazig City, Egypt

Mohamed Hussein,

El-tahlawy,

Abdelmoneim

et al. 2024

Zagazig Veterinary Journal

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The current study aimed to assess the prevalence of Enterococcus species isolated from some farmed fish species, including tilapia (Oreochromis niloticus), brush tooth lizard (Saurida undosquamis), horse mackerel (Trachurus trachurus), and red porgy (Pagrus pagrus) sold in Zagazig city, Sharkia Governorate, Egypt. Additionally, antimicrobial susceptibility testing was performed for Enterococcus species using the disc diffusion method. Furthermore, multiplex PCR was performed to identify virulence-associated genes of Enterococcus faecalis isolates. The results indicated contamination of the examined fish with various Enterococcus species with an overall total prevalence of 52.5%. The identified Enterococcus species were E. faecalis (26.25%), E. faecium (15%), and E. hirae, E. raffinosus, and E. durans (3.75%, each). The antibiotic sensitivity test revealed variable resistance patterns of the retrieved isolates to various antimicrobial agents, such as kanamycin (100%), clindamycin (76.9%), sulfamethoxazole (69.2%), ampicillin (69.2%), and colistin (61.5%). PCR screening of virulence genes revealed that E. faecalis harbored sodA (100%), gelE (83.3%), and ace (50%) genes. Consequently, urgent measures are needed to implement hygienic practices to control microbial contamination in both the aquatic environment and fish markets.

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Section: Disscussionmentioning

confidence: 75%

Prevalence, Antimicrobial Susceptibility, and Virulence Gene Profile of Enterococcus Species isolated from Some Farmed Fish Retailed in Zagazig City, Egypt

Mohamed Hussein,

El-tahlawy,

Abdelmoneim

et al. 2024

Zagazig Veterinary Journal

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“…The frequency of occurrence of enterococci in the analyzed fish samples was 58.1% (43/74). Igbinosa and Beshiru [ 10 ] reported a lower occurrence rate (8.19%) of these bacteria in ready-to-eat shrimp samples, meanwhile, Külahci and Gündoğan [ 29 ] found that over 21% of fresh fish were contaminated with enterococci. In this study, it was demonstrated that over 70% of the analyzed FS were contaminated with these bacteria at an average level of 1.48-log cfu/g (3.0×10 1 cfu/g).…”

Section: Discussionmentioning

confidence: 99%

Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland

Kukułowicz,

Steinka,

Gardocka

2024

PLoS ONE

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The growing concern over antibiotic resistance in foodborne pathogens necessitates comprehensive assessments of its prevalence and associated risks in various food products. The present study aimed to assess the occurrence of Enterococcus spp. in samples of fish purchased at various points of sale in the Tricity region. The selection of products (n = 74) was based on their availability and included both fish caught in the Baltic region and products imported from, Vietnam, China, Norway, and European Union (EU) countries. For bacterial isolation, samples were inoculated into selective broth, and the growth of enterococci was assessed based on turbidity. Positive cultures were confirmed by a change in color in bromocresol purple broth and were isolated on Slanetz-Bartley agar. Bacteria were present in all tested samples regardless of the degree of raw material processing as follows: frozen (F)– 55%, fresh/raw (FS)– 70.6%, thawed (DF)– 30%, smoked (S)– 50%, and the packaging methods, modified atmosphere packaging (MAP)– 34.4%, unit packaging (UP)– 75%, and sold in bulk (SB)– 76.9%, with an overall frequency of occurrence of 58.1%. The number of bacteria ranged from not detected to 4.28-log cfu/g, with the lowest mean values for thawed fish and those packed in MAP. Tests conducted on 24 strains isolated from samples showed their varied sensitivity to tetracyclines. Single cases of multidrug resistance of the tested strains were also observed. The conducted statistical analysis did not show statistically significant differences in the count of enterococci based on the origin, degree of processing, or packaging (p < 0.05). Moreover, differences in strain sensitivity to ampicillin were observed. Detected cases of resistance, especially to tetracycline, require careful monitoring and action to limit the health risks associated with resistant bacterial strains in food products.

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Phenotypic and genotypic characterization of Enterococcus faecalis and Enterococcus faecium isolated from fish, vegetables, and humans

Mubarak,

El-Zamkan,

Younis

et al. 2024

Sci Rep

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Enterococci, common hospital-acquired infections in immunocompromised patients, have garnered attention in clinical microbiology. To determine the clinical relevance of enterococci as food-borne pathogens, 116 fish, 90 vegetables, and 120 human diarrheal samples were tested for E. faecalis and E. faecium pathogenicity. Conventionally, 69 of 326 (21.17%) samples were positive for Enterococcus species, 52 (15.95%) of which were molecularly classified as E. faecalis and 13 (3.99%) as E. faecium. The E. faecalis contamination percentage of fresh fish (19.70%) was higher than frozen fish (4%). Cauliflower had the highest E. faecalis percentage (16.67%) when fish and vegetable samples didn’t harbor the E. faecium atpA gene. 23.33% and 10.83% of participants’ samples were molecularly confirmed as E. faecalis and E. faecium positive, respectively. E. faecalis isolates had all virulence genes, with gels being the most common (65.38%), while cylA and asa1 genes couldn’t be detected in E. faecium isolates. E. faecalis showed the highest resistance against vancomycin and tetracycline (69.23%), whereas E. faecium extremely resisted tetracycline (76.92%) and erythromycin (69.23%) with the recognition of MDR among 44.2% of E. faecalis and 38.5% of E. faecium isolates. The great similarity of our isolates showed the clinical importance of food-borne antibiotic-resistant enterococci.

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Occurrence and characteristics of staphylococci and enterococci in retail fish used for human consumption in Turkey

Cited by 3 publications

References 25 publications

Prevalence, Antimicrobial Susceptibility, and Virulence Gene Profile of Enterococcus Species isolated from Some Farmed Fish Retailed in Zagazig City, Egypt

Prevalence, Antimicrobial Susceptibility, and Virulence Gene Profile of Enterococcus Species isolated from Some Farmed Fish Retailed in Zagazig City, Egypt

Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland

Phenotypic and genotypic characterization of Enterococcus faecalis and Enterococcus faecium isolated from fish, vegetables, and humans

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