Occurrence and characterization of Escherichia coli ST410 co-harbouring blaNDM-5, blaCMY-42 and blaTEM-190 in a dog from the UK

Reynolds, Max; Phan, Hang; George, Sophie; Hubbard, Alasdair T. M.; Stoesser, Nicole; Maciuca, Iuliana E; Crook, Derrick W.; Timofte, Dorina

doi:10.1093/jac/dkz017

Cited by 33 publications

(22 citation statements)

References 20 publications

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“…In a recent study from Finland, bla NDM‐5‐ harbouring E. coli designated to ST167 has been identified in both humans and dogs (Grönthal et al, ). Meanwhile, bla NDM‐5 that belonged to ST410 was recently reported from dogs in South Korea (Hong et al, ) and the UK (Reynolds et al, ). In Egypt, E. coli ST410, co‐harbouring both bla NDM‐5 and bla OXA‐181 , has been identified from patients (Gamal et al, ).…”

Section: Discussionmentioning

confidence: 95%

“…However, the first identification of bla NDM‐5 E. coli from dogs in Northern Africa was reported in Algeria (Yousfi et al, ). Other studies have recently reported the isolation of bla NDM‐5 E. coli from dogs in Finland (Grönthal et al, ), South Korea (Hong et al, ) and the UK (Reynolds et al, ). Our analysis showed various STs linked to different NDM allelles in E. coli isolates from both sources.…”

Section: Discussionmentioning

confidence: 98%

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Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Ramadan

Gupta

Sharma

et al. 2019

Zoonoses and Public Health

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The emergence of NDM-producing Escherichia coli has considerably threatened human and animal health worldwide. This study describes for the first time in Egypt, the draft genome sequences of emerging NDM-5-producing E. coli from humans and dogs, and investigates genetic relatedness between isolates from both sources. Two E. coli from human urine and seven from environmental clinical samples of dogs exhibited resistance to carbapenems and harbouring bla NDM were subjected to Illumina Miseq whole-genome sequencing (WGS). Assembly and analysis of the reads were performed to identify resistance genes, multilocus sequence types (MLST), plasmid replicon types (Inc) and insertion sequences (IS) of the bla NDM region; core genome MLST (cgMLST) analysis was also performed. Two different NDM alleles were identified; bla NDM-5 in E. coli HR119 from the urine of a healthy person and environmental samples of dogs, and bla NDM-1 in E. coli HR135 from a human patient's urine. Multiple mobilizable resistance genes to different antimicrobial classes were identified except the colistin resistance gene, mcr. E. coli isolates from humans and dogs were assigned to different sequence types (STs). Using cgMLST, dog isolates clustered together with only 1-2 allellic differences; however, human E. coli showed 1,978 different allelles compared with dog isolates. Plasmidfinder results indicated the presence of an IncX3 replicon in bla NDM-5 -producing E. coli; however, bla NDM-1 was linked to IncCoIKP3.Notably, the NDM region (3 Kb) in all isolates from humans and dogs was highly similar with variable flanking sequences that represented different IS elements. This study reports the first emergence of NDM-5-producing E. coli from dogs in Egypt that shared some genetic features with human isolates and could be considered potential public health threats. K E Y W O R D S Egypt, Escherichia coli, insertion sequences, NDM-5, plasmid replicon, whole-genome sequencing | 325 RAMADAN et Al. This whole-genome shotgun project has been deposited at DDBJ/ ENA/GenBank under BioProject no. (PRJNA489683). ACK N OWLED G EM ENTSThe authors woulk like to thank the veterinarians in Zoo Care and Pet Clinic, Mansoura, Egypt, for their help during sample collection from animals. CO N FLI C T S O F I NTE R E S TNone. D ECL A R ATI O NThe mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information | 329 RAMADAN et Al. and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. O RCI D Hazem Ramadanhttps://orcid.org/0000-0002-6687-3758Charlene R. Jackson https://orcid.org/0000-0001-8480-4556 S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Ramadan

Gupta

Sharma

et al. 2019

Zoonoses and Public Health

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“…Analysis of the 24 strains using cgMLST confirmed that they were all highly related, all clustering in the same branch of the phylogenetic tree, in contrast to the sequences of seven other and independent E. coli ST410 from animal, environmental and human origin. The most closely related strain was a canine E. coli isolated in the UK in 2018, which carried the carbapenemase gene bla NDM-5 , the plasmid-mediated AmpC (pAmpC) cephalosporinase gene bla CMY-42 , and the β-lactamase gene bla TEM-190 [16] (GenBank accession number CP031653) (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Shedding of OXA-181 carbapenemase-producing Escherichia coli from companion animals after hospitalisation in Switzerland: an outbreak in 2018

et al. 2019

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BackgroundCarbapenem-resistant Enterobacteriaceae pose a serious threat to public health worldwide, and the role of companion animals as a reservoir is still unclear.AimsThis 4-month prospective observational study evaluated carriage of carbapenem-resistant Enterobacteriaceae at admission and after hospitalisation in a large referral hospital for companion animals in Switzerland.MethodsRectal swabs of dogs and cats expected to be hospitalised for at least 48 h were taken from May to August 2018 and analysed for the presence of carbapenem-resistant Enterobacteriaceae using selective agar plates. Resistant isolates were further characterised analysing whole genome sequences for resistance gene and plasmid identification, and ad hoc core genome multilocus sequence typing.ResultsThis study revealed nosocomial acquisition of Escherichia coli harbouring the carbapenemase gene bla OXA-181, the pAmpC cephalosporinase gene bla CMY-42 as well as quinolone resistance associated with qnrS1 and mutations in the topoisomerases II (GyrA) and IV (ParC). The bla OXA-181 and qnrS1 genes were identified on a 51 kb IncX3 plasmid and bla CMY-42 on a 47 kb IncI1 plasmid. All isolates belonged to sequence type ST410 and were genetically highly related. This E. coli clone was detected in 17 of 100 dogs and four of 34 cats after hospitalisation (21.6%), only one of the tested animals having tested positive at admission (0.75%). Two positive animals were still carriers 4 months after hospital discharge, but were negative after 6 months.ConclusionsCompanion animals may acquire carbapenemase-producing E. coli during hospitalisation, posing the risk of further dissemination to the animal and human population and to the environment.

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“…Whilst this may be a local phenomenon, it is interesting that many nations have reported increased detection of blaNDM-5 over the last few years. 15,16,17,18 Many isolates harboured other resistance mechanisms ( Figure S1, Table S1) and chromosomal mutations conferring high level fluoroquinolone resistance making many strains MDR ( Figure S1, Table S1). However, fosfomycin and chloramphenicol resistance was low (only 3 strains produced a full-length chloramphenicol resistance gene) suggesting that these antibiotics could be useful treatment options in this locale.…”

Section: Resultsmentioning

confidence: 99%

Human carriage of cefotaxime-resistant Escherichia coli in North-East India: an analysis of STs and associated resistance mechanisms

Paul

Babenko

Toleman

2019

Journal of Antimicrobial Chemotherapy

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Objectives To determine the prevalence of Escherichia coli STs and associated resistance mechanisms carried by the community in North-East India. Methods E. coli (108) were isolated from sewage collected from 19 sites across the city of Silchar by plating on MacConkey agar with/without selection (50 mg/L cefotaxime). Species identification was confirmed by MALDI-TOF MS for 82 isolates. Common resistance mechanisms were determined by WGS of pooled E. coli isolates. PFGE combined with specific probes determined the presence of common resistance mechanisms in all isolates. Phylotypes, multilocus STs, core-genome multilocus STs, resistance genes and virulence genes were determined by in silico analysis of 38 genomes. Results and conclusions Analysis of isolates collected without selection (n = 33) indicated that cefotaxime resistance in E. coli was 42% (14/33) and estimated meropenem resistance at 9%. The remaining 58% (19/33) were additionally susceptible to ampicillin, trimethoprim, ciprofloxacin and aminoglycosides. The most common ST among the cefotaxime-resistant E. coli was ST167 (29%), followed by ST410 (17%) and ST648 (10%). E. coli ST131 was absent from the collection. Sixty-three isolates were resistant to cefotaxime and harboured blaCTX-M-15 [54% (34/63)] or blaCMY-42 [46% (29/63)], of which 10% (6/63) harboured both genes. Carbapenem resistance was due to blaNDM-5, found in 10/63 cefotaxime-resistant isolates, and/or blaOXA-181, found in 4/63 isolates. NDM-5 was encoded by IncX3 and/or IncFII plasmids and CMY-42 was mostly encoded by IncI plasmids. NDM-5 appears to have replaced NDM-1 in this region and CMY-42 appears to be in the process of replacing CTX-M-15.

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Occurrence and characterization of Escherichia coli ST410 co-harbouring blaNDM-5, blaCMY-42 and blaTEM-190 in a dog from the UK

Cited by 33 publications

References 20 publications

Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Circulation of emerging NDM‐5‐producing Escherichia coli among humans and dogs in Egypt

Shedding of OXA-181 carbapenemase-producing Escherichia coli from companion animals after hospitalisation in Switzerland: an outbreak in 2018

Human carriage of cefotaxime-resistant Escherichia coli in North-East India: an analysis of STs and associated resistance mechanisms

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