Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces

Organji, Sameer R.; Abulreesh, Hussein H.; Elbanna, Кhaled; Osman, Gamal; Khider, Manal

doi:10.1016/j.apjtb.2015.04.004

Cited by 43 publications

(27 citation statements)

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“…This high resistance pattern could be due to irrational and widespread use of few antimicrobials. Agreeably, Organji et al (2015) from Egypt reported that 100% of tested isolates showed resistance to Penicillin G. Moreover, concurring the present finding, Organji et al (2015) reported that, 100% of tested isolates were susceptible to Erythromycin and Vancomycin.…”

Section: Discussionsupporting

confidence: 76%

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Bacillus Cereus isolation and load from raw cow milk sold in Markets of Haramaya District, eastern Ethiopia

et al. 2017

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Background: Bacillus cereus is a spore-forming bacterium that is widely distributed in the environment and can be detected in a variety of raw milk and milk products especially in vegetative form, which exposed directly in contact with the soil and produces toxins which caused food borne illness and considered as a significant public health hazard. A cross-sectional study was carried out to determine the prevalence and load of Bacillus cereus vegetative cells in raw bulk milk sold in market places at Bate, Haramaya and Aweday Towns, Eastern Ethiopia as well as to assess the in-vitro antimicrobial susceptibility of isolates. A total of 103 raw milk samples were collected and examined for the presence of Bacillus cereus by using Bacillus cereus selective agar media. The isolated presumptive colonies were further analyzed using standard microbiological methods to confirm the presence of Bacillus cereus. Spreading plate method was employed to count the bacteria from milk samples based on 10 fold serial dilutions. For antimicrobial susceptibility test, the disc diffusion test was employed using commercially available 10 antimicrobial discs. Results: The overall prevalence's of Bacillus cereus in raw milk samples was 38.8%. Milk collected from Aweday market showed higher occurrence (46.9%) than from Bate (40%) and Haramaya town (31.7%). Analysis of bacterial count showed that, 60% of Bacillus cereus positive milk samples were with bacterial load above recommended limit (>10

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Section: Discussionsupporting

confidence: 76%

“…The overall prevalence of B. cereus in raw bulk milk in Haramaya district was 38.8%. In their study Organji et al (2015) reported far higher prevalence (66.6%) of B. cereus isolated from of raw milk samples in Egypt. This variation could be due to the small sample size examined by the researchers.…”

Section: Discussionmentioning

confidence: 99%

Bacillus Cereus isolation and load from raw cow milk sold in Markets of Haramaya District, eastern Ethiopia

et al. 2017

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“…All the tested isolates were resistant to penicillin G, whereas sensitive to oxacillin, clindamycin, vancomycin, erythromycin, gentamicin, ciprofloxacin, and ceftriaxone. The data obtained herein with the others (Fenselau et al, 2008;Organji et al, 2015;Jawad et al, 2016) showed that B. cereus has a broad range of antibiotic susceptibility and validate the resistance to penicillin G by comparing to susceptibility to clindamycin, vancomycin, and erythromycin. Conclusion From the obtained data, many conclusions could be drawn, contamination of beef products with B. cereus increase the potential of foodborne infections among the consumers.…”

Section: Http://wwwopenveterinaryjournalcom R Shawish and R Tarabeesmentioning

confidence: 61%

Prevalence and antimicrobial resistance of <i>Bacillus cereus</i> isolated from beef products in Egypt

Shawish¹,

Tarabees²

2017

Open Vet J.

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Foodborne pathogens have the main concern in public health and food safety. Bacillus cereus food poisoning is one of the most important foodborne pathogens worldwide. In the present study, a total of 200 random beef product samples were collected from different supermarkets located at Menofia and Cairo governorates were examined for the presence of B. cereus. In addition, the presence of some virulence encoding genes was evaluated using Multiplex PCR. Finally, the antibiogram testing was conveyed to illustrate the resistance pattern of the confirmed B. cereus. The data showed that B. cereus was recovered from 22.5%, 30%, 25%, 37.5% and 15% of the minced meat, burger, sausage, kofta, and luncheon respectively. Among the 20 examined isolates 18/20 (90%) were harbor hblC enterotoxin encoding gene compared with 20/20 (100) were have cytK enterotoxin encoding gene. The isolated strains of B. cereus were resistant to penicillin G and sensitive to oxacillin, clindamycin, vancomycin, erythromycin, gentamicin, ciprofloxacin, and ceftriaxone. In all, the obtained data showed the importance of emerging B. cereus in disease control and prevention programs, and in regular clinical and food quality control laboratories in Egypt.

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“…cereus is undervalued as a foodborne pathogen, despite the fact that, it has been implicated in various foodborne outbreaks worldwide (Bennett et al, 2013) and this could be attributed to the lack of effective surveillance, underreporting of B. cereus linked food poisoning, and cases of B. cereus food poisoning apparently confused with Staphylococcus aureus and Clostridium perfringens food poisoning due to comparable symptoms (Fricker et al, 2007;StenforsArnesen et al, 2008), finally, B. cereus is not taken in account when diarrhea cases are diagnosed even if the fecal samples gave negative results for the presence of Salmonella, Shigella and Entamoeba in the middle east (Organji et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

El‐Zamkan¹,

Mubarak²

2017

AJVS

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Key words: B. cereus, Dairy desserts, Diarrhea and virulence genesB. cereus is an opportunistic foodborne pathogen that is found in most of the cases to be responsible for food-borne outbreaks. This study aimed to detect and quantify B. cereus in dairy desserts that locally manufactured and sold in different localities in Qena city, also to take an image about the role that is may be played by these products in causing foodborne illness by detection of B. cereus in diarrheal samples collected from the same regions from which the food samples are obtained. A total of 120 samples of ice-cream, mahallbia, rice and milk and diarrhea (30 samples each) were examined for presence of B. cereus. The results obtained in this study revealed detection of B. cereus in 55.6 and 40% of dairy dessert and diarrhea samples, respectively. B. cereus could be counted in 30, 60 and 76.7% of ice-cream, mahallbia and rice and milk samples with mean values of 3.5× 10 4 ±1.5× 10 4 , 6.3×10 5 ±1.9×10 5 and 1.4×10 7 ±6.6× 10 6 CFU/g, respectively. The possibility of involvement of theses dairy dessert samples in food poisoning cases was reinforced especially after detection the virulence genes of B. cereus isolates. It was found that 80, 44 and 24% of B. cereus isolates recovered from food samples harbored nhe, hbl and cytK genes, receptively including 55.6, 33.3 and 0% for ice-cream isolates; 83.3, 50 and 44.4% for mahallbia isolates and 86.9, 43.5 and 17.4% for rice and milk isolates. While for diarrhea samples, 75% of B. cereus isolates obtained from diarrhea samples carried nhe and cytK, respectively and 50% were found to be positive for hbl gene. Neither of the isolates that obtained from dairy dessert nor those obtained from diarrhea samples was positive for presence of ces gene.

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Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces

Cited by 43 publications

References 27 publications

Bacillus Cereus isolation and load from raw cow milk sold in Markets of Haramaya District, eastern Ethiopia

Bacillus Cereus isolation and load from raw cow milk sold in Markets of Haramaya District, eastern Ethiopia

Prevalence and antimicrobial resistance of <i>Bacillus cereus</i> isolated from beef products in Egypt

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

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