Occurrence and distribution of anisakid nematodes in Grey gurnard (Eutrigla gurnardus L.) from the North Sea

Haßler, Karl; Levsen, Arne

doi:10.1016/j.foodcont.2011.03.021

Cited by 31 publications

(17 citation statements)

References 19 publications

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“…A significant correlation was recorded between the abundance of A. pegreffii larvae present in the viscera of the hakes and the larvae counted in the flesh (r 2 = 0.345, p < 0.01). This result is consistent with similar observations reported by Karl and Levsen (2011) in grey gurnards infected by A. simplex (s. s.). Indeed, these authors reported in this fish "…a significantly positive relationship between both abundance and intensity of the larvae (A. simplex (s. s.)) in the flesh and the viscera (r = 0.64 and r = 0.59, respectively), i.e.…”

Section: Discussionsupporting

confidence: 94%

“…The hakes were gutted, and the viscera of each individual was separated. Flesh was cut into two to four portions of fillets, separating the hypaxial (ventral) and epaxial (dorsal) regions, following the horizontal septum, and afterwards it was placed in individual plastic bags, pressed under hydraulic press and stored overnight at −20°C for subsequent detection of larvae under UV light (Karl and Leinemann, 1993;Karl and Levsen, 2011;Levsen et al, in press). The larvae were counted and identified to genus level using an optical microscope according to the diagnostic morphological keys (sensu Berland, 1961).…”

Section: Parasitological Analysismentioning

confidence: 99%

“…So far, among the nine species of Anisakis genetically characterized (Mattiucci and Nascetti, 2008;Mattiucci et al, 2009), A. simplex (s. s.) and A. pegreffii are currently reported as zoonotic species causing human anisakiasis (D'Amelio et al, 1999;Umehara et al, 2007;Fumarola et al, 2009;Mattiucci et al, 2011Mattiucci et al, , 2013Mattiucci et al, , 2017bArai et al, 2014;Lim et al, 2015;Mladineo et al, 2016). In addition to the consumer health implications, anisakid nematodes also impact fish quality in terms of aesthetics, due to their repellent appearance when they occur in fish intended for human consumption (Karl and Levsen, 2011;Levsen and Karl, 2014).…”

Section: Introductionmentioning

confidence: 99%

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The Mediterranean European hake, Merluccius merluccius : Detecting drivers influencing the Anisakis spp. larvae distribution

et al. 2018

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A B S T R A C TThe European hake Merluccius merluccius is one of the most commercially important and widely distributed fish species, occurring both in European and Mediterranean Sea fisheries. We analyzed the distribution and infection rates of different species of Anisakis in M. merluccius (N = 1130 hakes), by site of infection in the fish host (viscera, dorsal and ventral fillets) from 13 different fishing grounds of the Mediterranean Sea (FAO area 37). The fillets were examined using the UV-Press method. A large number of Anisakis specimens (N = 877) were identified by diagnostic allozymes, sequence analysis of the partial EF1 α-1 region of nDNA and mtDNA cox2 gene. Among these, 813 larvae corresponded to A. pegreffii, 62 to A. physeteris, 1 to A. simplex (s. s.), whereas one resulted as a F1 hybrid between A. pegreffii and A. simplex (s. s.). Remarkably high levels of infection with A. pegreffii were recorded in hakes from the Adriatic/Ionian Sea compared to the fish of similar length obtained from the western Mediterranean fishing grounds. A positive correlation between fish length and abundance of A. pegreffii was observed. Concerning the localization of A. pegreffii larvae in the fish, 28.3% were detected in the liver, 62.9% in the rest of the viscera, 6.6% in the ventral part of the flesh, whereas 2.1% in the dorsal flesh.

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Section: Discussionsupporting

confidence: 94%

Section: Parasitological Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Mediterranean European hake, Merluccius merluccius : Detecting drivers influencing the Anisakis spp. larvae distribution

et al. 2018

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“…Muscle tissue was filleted and subsequently weighed (muscle weight, MW). Nematode larvae were removed from muscle tissue and visceral organs using the UV-press method of larval inspection as described by Karl & Leinemann [21] and later updated and modified by Karl & Levsen [22]. Nematode larvae were removed from all tissues and fixed in Davidsons' AFA fixative solution for 24 h before being stored in glycerol-alcohol.…”

Section: Parasitological Examinationmentioning

confidence: 99%

Increasing intensities of Anisakis simplex third-stage larvae (L3) in Atlantic salmon of coastal waters of Scotland

et al. 2020

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Background: Red Vent Syndrome (RVS), a haemorrhagic inflammation of the vent region in Atlantic salmon, is associated with high abundance of Anisakis simplex (s.s.) third-stage larvae (L3) in the vent region. Despite evidence suggesting that increasing A. simplex (s.s.) intensity is a causative factor in RVS aetiology, the definitive cause remains unclear. Methods:A total of 117 Atlantic salmon were sampled from commercial fisheries on the East, West, and North coasts of Scotland and examined for ascaridoid parasites. Genetic identification of a subsample of Anisakis larvae was performed using the internal transcribed spacer (ITS) region of ribosomal DNA. To assess the extent of differentiation of feeding grounds and dietary composition, stable isotope analysis of carbon and nitrogen was carried out on Atlantic salmon muscle tissue. Results:In the present study, the obtained ITS rDNA sequences matched A. simplex (s.s.) sequences deposited in GenBank at 99-100%. Not all isolated larvae (n = 30,406) were genetically identified. Therefore, the morphotype found in this study is referred to as A. simplex (sensu lato). Anisakis simplex (s.l.) was the most prevalent (100%) nematode with the highest mean intensity (259.9 ± 197.3), in comparison to Hysterothylacium aduncum (66.7%, 6.4 ± 10.2) and Pseudoterranova decipiens (s.l.) (14.5%, 1.4 ± 0.6). The mean intensity of A. simplex (s.l.) represents a four-fold increase compared to published data (63.6 ± 31.9) from salmon captured in Scotland in 2009. Significant positive correlations between A. simplex (s.l.) larvae intensities from the body and the vent suggest that they play a role in the emergence of RVS. The lack of a significant variation in stable isotope ratios of Atlantic salmon indicates that diet or feeding ground are not driving regional differences in A. simplex (s.l.) intensities. Conclusions:This paper presents the most recent survey for ascaridoid parasites of wild Atlantic salmon from three coastal regions in Scotland. A significant rise in A. simplex (s.l.) intensity could potentially increase both natural mortality rates of Atlantic salmon and possible risks for salmon consumers due to the known zoonotic role of A. simplex (s.s.) and A. pegreffii within the A. simplex (s.l.) species complex.

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“…Since the late 1980s, the grey gurnard has been thought to be responsible for the highest predation mortality among young Atlantic cod Gadus morhua, Norway pout Trisopterus esmarkii and whiting Merlangius merlangus (de Gee & Kikkert 1993). Grey gurnard catch rates in the international bottom trawl surveys have significantly increased in recent years (Floeter et al 2005), and the species is currently considered a sustainable stock, which permits the future increased use as a food resource (Horst & Levsen 2011).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and distribution of parasites in relation to food components of grey gurnard, Eutrigla gurnardus (L.) (Teleostei: Scorpaeniformes), off the Shetland Islands

Sobecka

Więcaszek

Łuczak

et al. 2014

Oceanological and Hydrobiological Studies

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The research was conducted on the parasite fauna and food composition of Eutrigla gurnardus caught as by-catch in commercial catches of the Atlantic cod Gadus morhua near the Shetland Islands. Thirteen species and two genera of pathogens were identified, including six species and one genus recorded for the first time in this host. Copepoda -Euphausiacea dominated in the stomach contents (they are also the intermediate hosts for most of the parasites found), while Gadidae dominated among the fish. A checklist of E. gurnardus parasites is included.

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Occurrence and distribution of anisakid nematodes in Grey gurnard (Eutrigla gurnardus L.) from the North Sea

Cited by 31 publications

References 19 publications

The Mediterranean European hake, Merluccius merluccius : Detecting drivers influencing the Anisakis spp. larvae distribution

The Mediterranean European hake, Merluccius merluccius : Detecting drivers influencing the Anisakis spp. larvae distribution

Increasing intensities of Anisakis simplex third-stage larvae (L3) in Atlantic salmon of coastal waters of Scotland

Occurrence and distribution of parasites in relation to food components of grey gurnard, Eutrigla gurnardus (L.) (Teleostei: Scorpaeniformes), off the Shetland Islands

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