Occurrence and distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in Morocco

Benseddik, Youssef; Joutei, Abdelmalek Boutaleb; Blenzar, Abdelali; Ezrari, Said; Molina, Carlos; Radouane, Nabil; Mokrını, Fouad; Tahiri, Abdessalem; Lahlali, Rachid; Dababat, Abdelfattah A.

doi:10.1080/09583157.2020.1787344

Cited by 26 publications

(9 citation statements)

References 39 publications

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“…The prevalence of Heterorhabditis and Steinernema was around 2.03%, and that of Oscheius was 7.35%, which was consistent with reports from the other regions. Globally, the prevalence of EPNs in soil samples was reported to range from 0.7 to 50% ( Benseddiq et al, 2020 ; Hominick, 2002 ) though specific sampling methods with repeated extraction technique may exceed this range ( Abd-Elgawad, 2020 ). Merits and demerits of nematode-sampling and extraction methods were recently reviewed ( Abd-Elgawad, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Sun

Zhang

Song³

et al. 2021

Journal of Nematology

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Spodoptera litura is a notorious leaf feeding insect pest in the Asia-Pacific region and leads to a significant economic loss in vegetable and field crop production. Entomopathogenic nematodes (EPNs), lethal parasites of insects, are used as biocontrol agents. Yunnan Province in China is a well-known region due to its rich biodiversity. In the present study, a survey of EPNs using the Galleria-baiting technique was conducted in 2017 and 2018 throughout the entire Yunnan province. In total, 789 soil samples were collected from 232 sites, of which 75 samples were positive for EPNs. Phylogenetic analyses of ITS, D2D3 expansion region of the 28S rRNA gene, as well as mitochondrial cytochrome c oxidase subunit I (COI), were performed to identify isolated nematode species and evaluate their genetic diversity. In total, 13, 3, and 58 identified populations belong to Steinernema, Heterorhabditis, and Oscheius, respectively. The phylogenetic relationships of EPN species in the three genera were analyzed with the Neighbor-Joining method. The virulence of the trapped isolates in the genera of Steinernema, Heterorhabditis, and Oscheius against S. litura was evaluated. Ten new indigenous isolates from Steinernema and Heterorhabditis showed prominent virulence to S. litura within 48 hr which is equivalent to that of commercial EPNs populations. The present study provides background information on indigenous EPN resources for S. litura control in Asia-Pacific region.

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Section: Discussionmentioning

confidence: 99%

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Sun

Zhang

Song³

et al. 2021

Journal of Nematology

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“…The literature on habitat preference for EPNs is various and contradictory. In some cases, soil samples collected from agricultural lands had more EPNs than those from natural habitats [ 36 , 59 , 60 , 61 ]. However, opposing results have also been found with EPNs more prevalent in natural habitats than agricultural ones [ 62 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Indigenous Entomopathogenic Nematodes as Potential Biocontrol Agents against Popillia japonica (Coleoptera: Scarabaeidae) in Northern Italy

Torrini

Paoli

Mazza

et al. 2020

Insects

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The natural presence of entomopathogenic nematodes (EPNs) has been investigated in the Piedmont region (Northern Italy) in areas infested by the Japanese beetle Popillia japonica. Thirty-nine out of 155 soil samples (25.2%) were positive for EPNs. Most of the samples contained only steinermatids (92.3%), 5.1% contained heterorhabditids, and one sample (2.6%) contained both genera. All the recovered isolates were identified at species level both morphologically and molecularly. Steinernema carpocapsae was the most abundant and it was mainly distributed in open habitats, such as perennial meadows, uncultivated soils, and cropland, characterized by sandy loam soil texture and acidic pH. Steinernema feltiae has been found associated mainly with closed habitats such as coniferous and deciduous woodland, characterized by sandy loam-texture and extremely acidic soil. The three isolates of Heterorhabditis bacteriophora were collected only in open habitats (perennial meadows and uncultivated fields) characterized by strongly acidic soils with sandy loam texture. The virulence of all EPN natural strains was evaluated by laboratory assays against P. japonica third-instar larvae collected during two different periods of the year (spring, autumn). The results showed that larval mortality was higher for pre-wintering larvae than post-wintering ones. The five more promising EPN isolates were tested in the semi-field assay in which H. bacteriophora natural strains have been shown to be more efficient in controlling P. japonica grubs. All of these results are finally discussed considering the use of these natural EPNs as biological control agents against P. japonica, within an eco-friendly perspective of management.

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“…Steinernema feltiae (Table 2), recently retrieved from soil in Morocco 21 , were used to infect susceptible late instar larvae of Galleria mellonella (Lepidoptera: Phyralidae). The EPN-killed G. mellonella larvae were transferred to White traps and then incubated at 25 °C until active infective juveniles (IJs) emerged 42,43 and daily collected from the White traps for 7 consecutive days and then were stored at 10 °C in a 0.5-L container filled with distilled water.…”

Section: Native Entomopathogenic Nematodes For the Trials Three Hetementioning

confidence: 99%

Potential of Moroccan entomopathogenic nematodes for the control of the Mediterranean fruit fly Ceratitis capitata Wiedemann (Diptera: Tephritidae)

Mokrını¹,

Laasli

Benseddik

et al. 2020

Sci Rep

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The Mediterranean fruit fly, Ceratitis capitata Wiedemann, is a deleterious pest worldwide affecting fruit production. The entomopathogenic nematodes (EPNs) are a potential biocontrol agent that could be effectively used to control this Mediterranean fruit fly. In this study, five EPN strains reported from different fields in Morocco were evaluated for their efficacy against C. capitata. In laboratory assays, Steinernema feltiae-SF-MOR9, S. feltiae-SF-MOR10 and Heterorhabditis bacteriophora-HB-MOR7 strains showed significantly higher infectivity and penetration rates when compared to the other strains. S. feltiae-SF-MOR9 caused the highest larval mortality rate (80%) at 50 infective juveniles (IJs) cm−2. However, additional results showed that both S. feltiae strains were significantly effective in controlling C. capitata larvae in apricot (Prunus armeniaca) fruits on soil surface with high mortality rate at 50 and 100 IJs cm−2. Different soil textures and moisture levels resulted in a significant variation in EPN strain virulence against C. capitata. Sandy clay loam soil in combination with 50 IJs cm−2 of S. feltiae (SF-MOR9 or SF-MOR10) caused a higher mortality rate of C. capitata larvae. Furthermore, applying these EPN strains at 50–100 IJs cm−2 in combination with 10–15% moisture level showed optimal results against C. capitata larvae. Therefore, those two Moroccan EPN strains could be used as promising eco-friendly biological agents against C. capitata.

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Occurrence and distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in Morocco

Cited by 26 publications

References 39 publications

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Evaluation of indigenous entomopathogenic nematodes in Southwest China as potential biocontrol agents against Spodoptera litura (Lepidoptera: Noctuidae)

Evaluation of Indigenous Entomopathogenic Nematodes as Potential Biocontrol Agents against Popillia japonica (Coleoptera: Scarabaeidae) in Northern Italy

Potential of Moroccan entomopathogenic nematodes for the control of the Mediterranean fruit fly Ceratitis capitata Wiedemann (Diptera: Tephritidae)

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