Occurrence and Distribution of Viruses Infecting Pepper in Korea

Choi, Gug-Seoun; Kim, Jae Hyun; Lee, Dong Hyuk; Kim, Jeong Soo; Ryu, Ki Hyun

doi:10.5423/ppj.2005.21.3.258

Cited by 52 publications

(29 citation statements)

References 5 publications

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“…The most common hot pepper diseases, which are responsible for economic yield losses of about 41%, are caused by viruses, such as Cucumber mosaic virus (CMV) (Kim et al, 2010). A total of 66 viruses have been reported in peppers worldwide, ten of which have been reported to infect Korean hot peppers (Choi et al, 2005;Kim et al, 1990). Many viruses in hot peppers are known to cause a wide range of symptoms such as mosaic, mottle, deformation, leaf spots, distortion, stunt, vein banding, necrosis, blistering and ring spots (Choi et al, 2005;Kim et al, 2010).…”

Section: Research Reportmentioning

confidence: 98%

Molecular and cytogenetic assessment of transgenic hot peppers resistant to Cucumber mosaic Virus

Lee

Choi

Ryu

et al. 2011

Hortic. Environ. Biotechnol.

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In this study, we have analyzed Cucumber mosaic virus (CMV)-resistant transgenic hot pepper (Capsicum annuum) plants harboring coat protein gene (CMVP0-CP) of the virus as a framework for molecular and cytogenetic assessment. PCR, Southern blot, RT-PCR, and Western blot techniques were applied to identify the existence and expression of the transgene in two independent transgenic hot pepper events in T4 generation, H15 and B20. Fluorescence in situ hybridization (FISH) was also conducted to detect physical location of the transgene on the chromosome of the plants. The amplified products of the inserted genes, 35S promoter with CMVP0-CP, CMVP0-CP, and NPT were obtained from the genomic DNA of the transgenic hot pepper lines by PCR. A single band, indicative of a single copy insertion, was observed by Southern blot analysis. Transcription of the transgene was confirmed from RT-PCR fragments CMVP0-CP and NPT genes in transgenic plants. Translated protein of the target gene, CMV-CP, was detected in the transgenic hot pepper events but not in nontransgenic control hot pepper lines by Western blot analysis. FISH analysis of transgenic line H15 revealed that the transgene was physically located at the sub-terminal portion of the long arm of chromosome d where the 5S rDNA locus is positioned. Conclusively, we observed that transgenes were maintained stably in T4 generation of transgenic hot peppers via cytogenetic investigation as well as molecular detection. These results can be useful in future studies conducted to evaluate breeding techniques and to assess genetically modified hot peppers.Additional key words: CMV, FISH, PCR, Southern blot, transgenic hot peppers, Western blot Hort. Environ. Biotechnol. 52(2):211-217. 2011.

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Section: Research Reportmentioning

confidence: 98%

Molecular and cytogenetic assessment of transgenic hot peppers resistant to Cucumber mosaic Virus

Lee

Choi

Ryu

et al. 2011

Hortic. Environ. Biotechnol.

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“…As opposed to PVY there are several reports displaying the evidence of the incidence of CMV which infects not only common vegetable crops but also some rare ornamental plants. A significant presence of CMV was demonstrated in other areas of pepper production in the world (Sepulveda et al, 2005;Choi et al, 2005a). According to many authors, CMV and PVY seem to be the most damaging viruses (Fakhfakh et al, 1999;Ben Khalifa et al, 2009).…”

Section: Disease Incidence and Symptomatology In The Fieldmentioning

confidence: 99%

Occurrence and diversity of viruses infecting pepper in Serbia

Milošević¹,

Ignjatov²,

Stanković³

et al. 2018

Acta agriculturae Serbica

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A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in subgroup N-1 with other European isolates of necrotic strains.

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“…Cucumber mosaic virus (CMV) is one of the important viral diseases in Korea and worldwide, infecting many different crop species including peppers (Choi et al, 2005;Palukaitis et al, 1992). Besides its importance, no complete resistances were found capable of restriction of CMV replication or long-distance movement in pepper.…”

Section: Introductionmentioning

confidence: 99%

“…In Korea, CMV was reported all province cultivating peppers (Choi et al, 2005). Some commercial cultivars, such as 'Bukang' have been used for the resistance sources to CMV in seed companies.…”

Section: Introductionmentioning

confidence: 99%

Developmental Changes of Recessive Genes-mediated Cucumber mosaic virus (CMV) Resistance in Peppers (Capsicum annuum L.)

Min¹,

Ryu²,

Ahn³

2014

HST

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Cucumber mosaic virus (CMV) is one of the most important viral diseases in pepper (Capsicum annuum L.), and several genes for resistance were reported in Capsicum spp. In Korea, a single dominant gene that is resistant to CMV Fny and CMV P0 has been used for breeding. Recently, a new strain (CMV P1) was reported that could infect cultivars resistant to both CMV Fny and CMV P0. Therefore, breeding of more robust CMV-resistant cultivars is required. In this study, we surveyed the inheritance of CMV P1 resistance and analyzed the location of the resistance loci. After CMV P1 inoculation of various germplasms and breeding lines, one accession (ICPN18-8) showed no visual symptoms at 15 dpi (days post inoculation) but was susceptible after 45 dpi, and one resistant line (I7339) showed resistance until at 45 dpi. The latter line was used for tests of resistance inheritance. A total of 189 F2 plants were examined, with 42 individuals showing resistance at 15 dpi and a phenotype segregation ratio close to 1:3 (resistant:susceptible plants). In a lateral ELISA test at 45 dpi, 11 plants showed resistance, and the segregation ratio was changed to 1:15. These results indicate that resistance in C. annuum 'I7339' is controlled by two different recessive genes; we named these resistance genes 'cmr3E' and 'cmr3L,' respectively. To locate these two resistant loci in the pepper linkage map, various RAPD, SSR, and STS markers were screened; only nine markers were grouped into one linkage group (LG). Only one RAPD primer (OPAT16) was distantly linked with cmr3E (22.3 cM) and cmr3L (20.7 cM). To develop more accurate markers for marker-assisted breeding, enriching for molecular markers spanning two loci will be required. Additional key words: linkage group, RAPD

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Occurrence and Distribution of Viruses Infecting Pepper in Korea

Cited by 52 publications

References 5 publications

Molecular and cytogenetic assessment of transgenic hot peppers resistant to Cucumber mosaic Virus

Molecular and cytogenetic assessment of transgenic hot peppers resistant to Cucumber mosaic Virus

Occurrence and diversity of viruses infecting pepper in Serbia

Developmental Changes of Recessive Genes-mediated Cucumber mosaic virus (CMV) Resistance in Peppers (Capsicum annuum L.)

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