Occurrence and features of chromosomally encoded carbapenemases in Gram-negative bacteria in farm animals sampled at slaughterhouse level

Zurfluh, Katrin; Hindermann, Denise; Nüesch‐Inderbinen, Magdalena; Poirel, Laurent; Nordmann, Patrice; Stephan, Roger

doi:10.17236/sat00072

Cited by 8 publications

(10 citation statements)

References 14 publications

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“…Livestock may be an important vehicle for the community-wide dissemination of antimicrobial-resistant Enterobacteriaceae, also P. aeruginosa especially ESBL-producing type isolates have been found in increasing numbers in food-producing animals [ 9 , 10 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…The prevalence of MDR enterobacteriaceae in slaughterhouses, including swine and poultry environments, has been reported in several studies building a growing alarm about their effect on animal and human health [ 7 , 8 ]. Recently encountered the emergence of livestock associated ESBL-producing P. aeruginosa in cow, poultry and pigs [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa in camel in Egypt: potential human hazard

Elhariri

Hamza

Elhelw

et al. 2017

Ann Clin Microbiol Antimicrob

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BackgroundThe rapid increase of extended-spectrum beta-lactamase (ESBL) producing bacteria are a potential health hazard. Development of antimicrobial resistance in animal pathogens has serious implications for human health, especially when such strains could be transmitted to human. In this study, the antimicrobial resistance due to ESBL producing Pseudomonas aeruginosa in the camel meat was investigated.MethodsIn this study meat samples from 200 healthy camels at two major abattoirs in Egypt (Cairo and Giza) were collected. Following culture on cetrimide agar, suspected P. aeruginosa colonies were confirmed with a Vitek 2 system (bioMe´rieux). P. aeruginosa isolates were phenotypically identified as ESBL by double disk synergy test. Additionally antimicrobial susceptibility testing of ESBL producing P. aeruginosa isolates were done against 11 antimicrobial drugs and carried out by disk diffusion method. The ESBL genotypes were determined by polymerase chain reaction according to the presence of the bla PER-1, bla CTX-M, bla SHV, and bla TEM.Results Pseudomonas aeruginosa was isolated from 45 camel meat sample (22.5%). The total percentage of ESBL producing P. aeruginosa was 45% (21/45) from camel meat isolates. Antibiogram results revealed the highest resistance was for c, ceftriaxone and rifampicin followed by cefepime and aztreonam. The prevalence rates of β-lactamase genes were recorded (bla PER-1 28.5%, bla CTX-M 38%, bla SHV 33.3% and bla TEM 23.8%).ConclusionsThis study illustrates the presence of high rates of ESBL-P. aeruginosa in camels that represents an increasing alarming for the risk of transmission to human and opens the door for current and future antibiotics therapy failure. Livestock associated ESBL-P. aeruginosa is a growing disaster, therefore, attention has to be fully given to livestock associated ESBL-bacteria which try to find its way to human beings.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa in camel in Egypt: potential human hazard

Elhariri

Hamza

Elhelw

et al. 2017

Ann Clin Microbiol Antimicrob

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“…Aside from clinical settings, the carbapenemase enzymes OXA, VIM, NDM, and KPC possess the highest impact in wildlife, pets, and the food chain ( Grundmann et al, 2017 ; Köck et al, 2018 ). In Germany, France, Italy, Spain, and the Netherlands, reports have been published about carbapenem-resistant Enterobacteriaceae (CRE) in companion animals (i.e., dogs, cats, horses) ( Stolle et al, 2013 ; Schmiedel et al, 2014 ; Gonzalez-Torralba et al, 2016 ; Melo et al, 2017 ; Vittecoq et al, 2017 ; Pulss et al, 2018 ), seafood (i.e., shrimps, blue mussels, cockles) ( Roschanski et al, 2016 ; Ceccarelli et al, 2017 ), wild animals (i.e., yellow-legged gulls, black kite) ( Fischer et al, 2013b ; Vergara et al, 2017 ; Vittecoq et al, 2017 ), and food of livestock animals (i.e., beef, chicken meat, pork) ( Schwaiger et al, 2008 ; Leverstein-van Hall et al, 2011 ; Poirel et al, 2012 ; Zurfluh et al, 2016 ; Ceccarelli et al, 2017 ; Fischer et al, 2017 ; Pulss et al, 2017 ; Randall et al, 2017 ; Roschanski et al, 2017 , 2018 ). In 2011, VIM-1-producing Enterobacteriaceae ( Salmonella Infantis and E. coli ) were found in several chicken and pig farms in Germany ( Borowiak et al, 2017 ; Falgenhauer et al, 2017 ; Fischer et al, 2017 ; Irrgang et al, 2017 , 2019 ; Roschanski et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

ChromID® CARBA Agar Fails to Detect Carbapenem-Resistant Enterobacteriaceae With Slightly Reduced Susceptibility to Carbapenems

et al. 2020

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Validation of Selective CPE Agar Our data are in concordance with the results of eleven state laboratories that had participated in this study with their ChromID R CARBA batches routinely used for the German CPE monitoring. Based on the determined CPE detection rate, we recommend the use of McC+CTX+MEM for monitoring purposes. This study indicates that the use of ChromID R CARBA agar might lead to an underestimation of the current CPE occurrence in food and livestock samples.

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“…The use of carbapenems is prohibited in food-producing animals in all countries (OIE, 2015; Webb et al, 2016). Notwithstanding, scientific studies reporting CPE and CEG in livestock and their environment are progressively more frequent (Guerra et al, 2014; Webb et al, 2016; Zurfluh et al, 2016; Fischer et al, 2017). Furthermore, in the last EFSA report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2015, the presumptive extended-spectrum beta-lactamase (ESBL)-/AmpC-/carbapenemase-production in Salmonella and E. coli was monitored in humans, meat (pork and beef), fattening pigs and calves for the first time (EFSA and ECDC, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Although the occurrence of carbapenemases was first discovered and mainly investigated at hospitals and healthcare facilities, scientific studies reporting carbapenemase-producers, and carbapenemase-encoding genes (CEG) in animals, the environment and food are increasingly frequent. Specifically, carbapenem resistance has been detected in livestock and in their environments in France, Germany, Switzerland, the USA, and China (Guerra et al, 2014; Webb et al, 2016; Zurfluh et al, 2016; Fischer et al, 2017); companion animals and wildlife (Guerra et al, 2014; Woodford et al, 2014); aquatic environments (Zurfluh et al, 2013; Guerra et al, 2014; Woodford et al, 2014; Fernando et al, 2016); retail chicken meat from Egypt (Abdallah et al, 2015); vegetables and seafood from Asia, India and Brazil (Guerra et al, 2014; Morrison and Rubin, 2015; Zurfluh et al, 2015, 2016). This suggests that non-human sources may be reservoirs of CPE and CEG.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR

et al. 2018

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In this study, 30 samples of processed edible mealworms (Tenebrio molitor L.) and 30 samples of grasshoppers (Locusta migratoria migratorioides) were obtained from producers located in Europe (Belgium and the Netherlands) and Asia (Thailand) and subjected to PCR-DGGE analyses. The PCR-DGGE analyses showed that species in the genus Staphylococcus were predominant in the samples of mealworms from Belgium and grasshoppers from the Netherlands; species in the genus Bacillus were detected in the samples of mealworms and grasshoppers from Thailand. Moreover, Weissella cibaria/confusa/spp. was found in grasshoppers from Belgium. Since data concerning the role of novel foods such as edible insects in the dissemination of carbapenem resistance are currently lacking, the quantification of five carbapenemase encoding genes (blaNDM−1, blaVIM, blaGES, blaOXA−48, and blaKPC) by qPCR was also carried out in all the samples under study. The genes coding for GES and KPC were not detected in the analyzed samples. A very low frequency of blaOXA−48 (3%) and blaNDM−1 (10%) genes was detected among mealworms. In contrast, grasshoppers were characterized by a high incidence of the genes for OXA-48 and NDM-1, accounting for 57 and 27% of the overall grasshopper samples, respectively. The blaVIM gene was detected exclusively in two grasshopper samples from Thailand, showing only 7% positivity. The analysis of variance showed that all the effects (producers, species, and producers × species) were statistically significant for blaNDM−1, whereas for blaOXA−48 and blaVIM, no significant effects were detected for the same source of variation. Further studies are necessary to assess the possible role of edible insects as reservoirs for the resistance to carbapenems and to understand the correlation with the insect microbiota. Furthermore, an intensified surveillance plan examining the occurrence of carbapenemase encoding genes in the food chain and in environmental compartments is needed for a proper risk assessment. In such a context, the appropriate use of antimicrobials represents the main preventive action that should always be applied.

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Occurrence and features of chromosomally encoded carbapenemases in Gram-negative bacteria in farm animals sampled at slaughterhouse level

Cited by 8 publications

References 14 publications

Extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa in camel in Egypt: potential human hazard

Extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa in camel in Egypt: potential human hazard

ChromID® CARBA Agar Fails to Detect Carbapenem-Resistant Enterobacteriaceae With Slightly Reduced Susceptibility to Carbapenems

Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR

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