Occurrence and genetic diversity of Enterocytozoon bieneusi (Microsporidia) in owned and sheltered dogs and cats in Northern Spain

Dashti, Alejandro; Santı́n, Mónica; Cano, Lourdes; Lucio, Aida de; Bailo, Begoña; Mingo, Marta Hernández de; Köster, Pamela C.; Fernández-Basterra, José A.; Aramburu-Aguirre, Juan; López-Molina, Nuria; Fernández-Crespo, Juan C.; Calero‐Bernal, Rafael; Carmena, David

doi:10.1007/s00436-019-06428-1

Cited by 42 publications

(34 citation statements)

References 55 publications

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“…In the present study, genotype Peru11, a zoonotic genotype, was identified in pet fancy rats for the first time. This genotype has been found previously in humans in Peru, China and Thailand, non-human primates in Kenya and China, raccoons, voles and cottontails in the United States, chickens in Brazil, cats in Spain, and minks and water in China [4,5,17,33]. Genotype SCC-2 was reported previously in pet chipmunks and squirrels in China [6,8], and was found in pet fancy rats for the first time.…”

Section: Resultssupporting

confidence: 59%

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Wang

Zhao

et al. 2020

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Enterocytozoon bieneusi, an obligate intracellular microsporidian parasite, can infect humans and a wide variety of animals worldwide. However, information on the prevalence and molecular characterization of E. bieneusi in pet rats and guinea pigs is lacking. In this study, 325 fecal samples were collected from 152 pet fancy rats and 173 pet guinea pigs purchased from pet shops in Henan and Shandong provinces. The prevalence of E. bieneusi was 11.2% (17/152) in pet fancy rats and 20.2% (35/173) in pet guinea pigs. Genotypes D (n = 12), Peru11 (n = 3), S7 (n = 1) and SCC-2 (n = 1) were identified in pet fancy rats, and genotype S7 (n = 30) and a novel genotype PGP (n = 5) were identified in pet guinea pigs. The ITS sequence and its phylogenetic analysis showed that the novel genotype PGP was distinctly different; it exhibited less than 50% similarity to the reference sequences, and did not cluster with any of the known E. bieneusi genotype groups, forming a unique branch between groups 6 and 7. These data suggest that this is a new E. bieneusi genotype group. This is the first report of E. bieneusi infection in pet fancy rats and pet guinea pigs worldwide. The identification of zoonotic genotypes D, Peru11, and S7 suggests that pet fancy rats and guinea pigs can be potential sources of human microsporidiosis.

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Section: Resultssupporting

confidence: 59%

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Wang

Zhao

et al. 2020

Parasite

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“…Infections with E. bieneusi in dogs and cats have been reported in Spain, Brazil, China, Colombia, Germany, Japan, Portugal, Switzerland, Poland, Turkey, Iran, the Czech Republic, Egypt, and Thailand, where they range from 0% to 25.8% in dogs and from 1.4% to 31.3% in cats [7,9,[14][15][16][18][19][20][21][22][23]. In the present study, 149 dogs (22.9%) and 79 cats (20.3%) were E. bieneusi-positive by nested PCRbased sequencing of the ITS region, a somewhat higher rate than those reported in most previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…18), GD1* (10), GD2(19), GD3 (2), GD4 (2), (1), EbpC(7), CD9 (4), I (2) No 83 PtEb IX(38), GD1* (28), GD2 (5), D (12) Total 149…”

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confidence: 99%

Occurrence, risk factors and genotypes of Enterocytozoon bieneusi in dogs and cats in Guangzhou, southern China: high genotype diversity and zoonotic concern

Wang¹,

Lin

Sun

et al. 2020

Preprint

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Background: Enterocytozoon bieneusi , a common opportunistic fungal pathogen, has a wide range of hosts. Limited epidemiological data on E. bieneusi intestinal infections in companion animals (dogs and cats) in China exists. In this study, fecal samples (651 from dogs and 389 from cats) in Guangzhou city, Guangdong Province, China, were collected, and the ribosomal internal transcribed spacer region from the DNA extracted from them was PCR-amplified and sequenced. Results: Based on the sequencing data, E. bieneusi was identified in the fecal samples collected from 149 (22.9%) and 79 (20.3%) dogs and cats. Of the factors investigated, poor living conditions appeared be the major risk factor for contracting the pathogen. Eleven E. bieneusi genotypes, six known (PtEb IX, GD1, D,CD9 , EbpC, I) and five novel (designated here as GD2– GD6), were found in dogs. Eight genotypes, six known (PtEb IX, GD1, D, CD9, EbpC, Type IV) and two novel (GD2 and GC1), were identified in cats. Genotype PtEb IX was most common in both dogs and cats, followed by genotype GD1. Conclusions: Although PtEb IX was the most common E. bieneusi genotype in dogs, this is the first report of this genotype dominating in cats. The same genotype distribution of the pathogen between the two different companion animals species in the same geographic area indicates that inter-species transmission is probable. The widespread existence of zoonotic E. bieneusi genotypes (D, EbpC, Type IV) in companion animals indicates that they are potential sources of environmental contamination and infections in humans.

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“…Infections with E. bieneusi in dogs and cats have been reported in Spain, Brazil, China, Colombia, Germany, Japan, Portugal, Switzerland, Poland, Turkey, Iran, the Czech Republic, Egypt, and Thailand, where they range from 0% to 25.8% in dogs and from 1.4% to 31.3% in cats [7,9,[14][15][16][18][19][20][21][22][23]. In the present study, 149 dogs (22.9%) and 79 cats (20.3%) were E. bieneusi-positive by nested PCR-based sequencing of the ITS region, a somewhat higher rate than those reported in most previous studies.…”

Section: Discussionmentioning

confidence: 99%

Occurrence, risk factors and genotypes of Enterocytozoon bieneusi in dogs and cats in Guangzhou, southern China: high genotype diversity and zoonotic concern

Wang¹,

Lin

Sun

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Enterocytozoon bieneusi, a common opportunistic fungal pathogen, has a wide range of hosts. Limited epidemiological data on E. bieneusi intestinal infections in companion animals (dogs and cats) in China exists. In this study, fecal samples (651 from dogs and 389 from cats) in Guangzhou city, Guangdong Province, China, were collected, and the ribosomal internal transcribed (ITS) spacer region from the DNA extracted from them was Polymerase Chain Reaction (PCR)-amplified and sequenced.Results: Based on the sequencing data, E. bieneusi was identified in the fecal samples collected from 149 (22.9%) and 79 (20.3%) dogs and cats. Of the factors investigated, poor living conditions appeared to be the major risk factor for contracting the pathogen. Eleven E. bieneusi genotypes, six known (PtEb IX, GD1, D, CD9, EbpC, I) and five novel (designated here as GD2– GD6), were found in dogs. Eight genotypes, six known (PtEb IX, GD1, D, CD9, EbpC, Type IV) and two novel (GD2 and GC1), were identified in cats. Genotype PtEb IX was most common in both dogs and cats, followed by genotype GD1.Conclusions: Although PtEb IX was the most common E. bieneusi genotype in dogs, this is the first report of this genotype dominating in cats. The same genotype distribution of the pathogen between the two different companion animals species in the same geographic area indicates that inter-species transmission is probable. The widespread existence of zoonotic E. bieneusi genotypes (D, EbpC, Type IV) in companion animals indicates that they are potential sources of environmental contamination and infections in humans.

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Occurrence and genetic diversity of Enterocytozoon bieneusi (Microsporidia) in owned and sheltered dogs and cats in Northern Spain

Cited by 42 publications

References 55 publications

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Occurrence, risk factors and genotypes of Enterocytozoon bieneusi in dogs and cats in Guangzhou, southern China: high genotype diversity and zoonotic concern

Occurrence, risk factors and genotypes of Enterocytozoon bieneusi in dogs and cats in Guangzhou, southern China: high genotype diversity and zoonotic concern

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