Occurrence and genotype characterization of Giardia duodenalis in goat kids from the Canary Islands, Spain

Ruiz, Antonio; Foronda, Pilar; González, J.; Guedes, A.; Abreu-Acosta, Néstor; Molina, José Molina; Valladares, Basilio

doi:10.1016/j.vetpar.2008.03.003

Cited by 45 publications

(44 citation statements)

References 23 publications

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“…All sequences were deposited in GenBank under accession numbers KC122265, JX876559-JX876565. Isolates C2, C12 and C15 showed 100% identity to the published sequences from genotype E isolates obtained from goats in Spain (Ruiz et al 2008). Similarly, isolates C1, C3, C4, C14 and C16 showed 100% identity to the published sequences from genotype E also obtained from goats in Spain (Ruiz et al 2008).…”

Section: Resultsmentioning

confidence: 69%

“…Isolates C2, C12 and C15 showed 100% identity to the published sequences from genotype E isolates obtained from goats in Spain (Ruiz et al 2008). Similarly, isolates C1, C3, C4, C14 and C16 showed 100% identity to the published sequences from genotype E also obtained from goats in Spain (Ruiz et al 2008). Moreover, a BLAST analysis showed 100% similarity between these isolates and genotype E samples obtained from sheep in Mexico (Di Giovanni et al 2006) and in Spain (Gomez-Munoz et al 2009).…”

Section: Resultsmentioning

confidence: 69%

“…All sequences were deposited in GenBank under accession numbers KF483113-KF483115. Isolates C2, C12 and C14 showed 100% identity to the GenBank published sequences of genotype E obtained from goats in Spain (Ruiz et al 2008), as well as one isolate obtained from a goat in Malaysia (HQ283233 - Lim et al 2013). Moreover, a BLAST search showed a 100% identity match with genotype E isolate obtained from cows in the USA (Feng et al 2008).…”

Section: Resultsmentioning

confidence: 84%

“…Giardia duodenalis is subdivided into eight different genotypes (A-H), but only genotypes A, B and E have been reported in goats (Castro-Hermida et al 2007;Geurden et al 2008;Ruiz et al 2008;Berrili et al 2012;Lim et al 2013).…”

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confidence: 99%

“…The role of goats in the zoonotic transmission of Giardia duodenalis is controversial because genotype E, which is not considered zoonotic, is more common than the zoonotic genotypes A and B. However, only a few molecular studies regarding Giardia prevalence in goats have been performed worldwide (CastroHermida et al 2007;Geurden et al 2008;Ruiz et al 2008;Berrili et al 2012;Lim et al 2013), although a large number of studies have been conducted in other ruminant hosts (reviewed by Feng and Xiao 2011).…”

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confidence: 99%

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First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

Sudré¹,

Leles²,

Lima³

et al. 2014

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to perform the first molecular genotyping of Giardia duodenalis from goats in Brazil, in order to assess the risk for zoonotic transmission. Samples were collected from two dairy goat farms (Saanen breed) located in the city of Niteroi in the state of Rio de Janeiro, Brazil. Goat faecal samples (n = 58) were collected directly from the rectums of all animals up to one year of age and were subjected to centrifuge-flotation in sugar saturate solution. Nested-PCR and sequencing using β-giardin and tpi gene targets was performed on positive samples. Seventeen out of fifty-eight (29.31%) faecal samples were positive for Giardia duodenalis cysts, all belonging to the same farm. Only eight isolates were successfully sequenced (eight samples for β-giardin and four samples for tpi), all belonging to genotype E. Two types of sequences were identified for each locus within isolates from the current study, which exhibited sequence heterogeneity with variable numbers of single nucleotide polymorphisms. The present study contributes to a better understanding of the molecular epidemiology of this parasite.

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Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 84%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

Sudré¹,

Leles²,

Lima³

et al. 2014

Vet. Med. - Czech

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Analysis of nucleotide variation within the triose‐phosphate isomerase gene ofGiardia duodenalisfrom sheep and its zoonotic implications

et al. 2010

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This study explored the genetic composition of Giardia in fecal samples from 284 individual lambs on pasture-based sheep farms in three regions of Victoria, Australia. An approach, combining targeted sequencing, phylogenetic analysis and PCR-coupled restriction endonuclease fingerprinting, was used to identify and genetically categorize Giardia present in 43 (15.1%) of the 284 samples and to infer their zoonotic potential. The specific identity and genetic classification were based on the phylogenetic analysis of sequence data for a portion of the triose-phosphate isomerase gene. Fourteen different sequence variants (including seven sequences that contained between one and five polymorphic sites) representing two distinct assemblages of Giardia (recognized in the current literature) were defined, of which 13 were new records. One dominant sequence type (with accession no. GQ444447, representing a genotype within assemblage A) has been detected previously in humans and is thus considered to be of zoonotic potential. (Nucleotide sequences reported in this article are available in the GenBank database under accession nos. GQ444447-GQ444451 and GQ444454-GQ444462).

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Giardia in Pets and Farm Animals, and Their Zoonotic Potential

Geurden

Olson

2011

Giardia

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Occurrence and genotype characterization of Giardia duodenalis in goat kids from the Canary Islands, Spain

Cited by 45 publications

References 23 publications

First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

Analysis of nucleotide variation within the triose‐phosphate isomerase gene ofGiardia duodenalisfrom sheep and its zoonotic implications

Giardia in Pets and Farm Animals, and Their Zoonotic Potential

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