Occurrence and identification of grapevine phytoplasmas in main viticultural regions of Turkey

Ertunç, Filiz; Orel, Didem Canik; Bayram, Şerife; Paltrinieri, Samanta; Bertaccini, Assunta; Topkaya, Şerife; Söylemezoğlu, Gökhan

doi:10.1007/s12600-014-0449-7

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…Interestingly, group 16SrI phytoplasmas related to those causing AY disease in herbaceous plants have been reported in diseased V. vinifera in several regions of the world. In addition to North America, grapevine yellows-associated phytoplasmas belonging to subgroup 16SrI-B have been reported in South America, Europe, and Africa (Alma et al 1996;Engelbrecht et al 2010;Ertunc et al 2015;Gajardo et al 2009;M'hirsi et al 2004;Olivier et al 2009Olivier et al , 2014) (this study). The design and implementation of effective strategies to manage these diseases will rest, in part, upon knowledge of non-V. vinifera plant hosts and insect vectors of these phytoplasmas.…”

Section: Discussionmentioning

confidence: 51%

Genotyping Points to Divergent Evolution of ‘CandidatusPhytoplasma asteris’ Strains Causing North American Grapevine Yellows and Strains Causing Aster Yellows

et al. 2018

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Grapevine yellows diseases occur in cultivated grapevine (Vitis vinifera L.) on several continents, where the diseases are known by different names depending upon the identities of the causal phytoplasmas. In this study, phytoplasma strains associated with grapevine yellows disease (North American grapevine yellows [NAGY]) in vineyards of Pennsylvania were characterized as belonging to 16S ribosomal RNA (rRNA) gene restriction fragment length polymorphism group 16SrI (aster yellows phytoplasma group), subgroup 16SrI-B (I-B), and variant subgroup I-B*. The strains (NAGYI strains) were subjected to genotyping based on analyses of 16S rRNA and secY genes, and to in silico three-dimensional modeling of the SecY protein. Although the NAGYI strains are closely related to aster yellows (AY) phytoplasma strains and are classified like AY strains in subgroup I-B or in variant subgroup I-B*, the results from genotyping and protein modeling may signal ongoing evolutionary divergence of NAGYI strains from related strains in subgroup 16SrI-B.

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Section: Discussionmentioning

confidence: 51%

Genotyping Points to Divergent Evolution of ‘CandidatusPhytoplasma asteris’ Strains Causing North American Grapevine Yellows and Strains Causing Aster Yellows

et al. 2018

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“…P. phoenicium’‐related strains obtained showed 99% identity with the one reported recently from grapevine in Iran, (GenBank Accession Number ) (Salehi, Salehi et al., ). These phytoplasmas were also detected in grapevine in Turkey (Canik, Ertunc, Paltrinieri, Contaldo, & Bertaccini, ; Ertunc et al., ).…”

Section: Discussionmentioning

confidence: 93%

“…The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches (Felsenstein, 1985); only values above 50 are reported strains obtained showed 99% identity with the one reported recently from grapevine in Iran, (GenBank Accession Number KX011516) . These phytoplasmas were also detected in grapevine in Turkey (Canik, Ertunc, Paltrinieri, Contaldo, & Bertaccini, 2011;Ertunc et al, 2015).…”

Section: Discussionmentioning

confidence: 95%

Molecular identification of diverse ‘Candidatus Phytoplasma’ species associated with grapevine decline in Iran

Zamharir

Paltrinieri

Hajivand

et al. 2017

Journal of Phytopathology

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Grapevine (Vitis vinifera) is one of the most important fruits in Iran where the provinces of Qazvin, Lorestan and Markazi are main producers. During 2013–2015, vineyards located in these provinces were surveyed to verify the presence of phytoplasma. The sample collection was based on symptomatology including decline, leaf yellowing and shortening of internodes. Total DNA was extracted from symptomatic and symptomless grapevine samples and used in nested‐polymerase chain reaction (PCR) assays with phytoplasma ribosomal primers (P1/Tint followed by R16F2n/R2, R16mF1/mR1, R16(I)F1/R1 or 6R758f/16R1232r). Nested‐PCR products were obtained only for symptomatic samples while samples from symptomless plants yielded no PCR products. Restriction fragment length polymorphism (RFLP) analyses with Tru1I, TaqI and Tsp509I and direct sequencing of amplicons followed by phylogenetic analyses indicated the presence of ‘Candidatus Phytoplasma fraxini’, ‘Ca. P. aurantifolia’, ‘Ca. P. solani’ and ‘Ca. P. phoenicium’‐related strains. In Marzaki province, there ‘Ca. P. aurantifolia’ strains were mainly detected, while in the other two provinces, all the four ‘Candidatus species’ were identified with the prevalence of ‘Ca. P. solani’‐related strains. In both provinces in one case, mixed phytoplasma infection was also detected by RFLP analyses. The presence of different phytoplasmas in positive samples indicates great phytosanitary significance due to grapevine economic importance for country. Grapevine phytoplasma infection represents a threat for other crops suggesting grapevine as alternative host species for the phytoplasmas already reported in Iran, while the ‘Ca. P. fraxini’ is for the first time identified in Iran.

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“…In recent years, numerous phytoplasma survey studies have been completed in economically important crops and on their surrounding weeds in different geographical regions of Turkey (Alp et al 2016;Oksal et al 2017;Kaçar et al 2019;Gazel et al 2020;Oksal 2020). These surveys revealed the presence of phytoplasma agents belonging to different phytoplasma groups including 16Sr I (Aster yellows), II (Peanut witches' broom), V (Elm yellows), VI (Clover proliferation), IX (Pigeon pea witches' broom), X (Apple proliferation), XII (Stolbur), and XIV (Bermudagrass white leaf ) (Canik & Ertunç 2007;Ertunc et al 2015;Özdemir & Çağırgan 2015;Usta et al 2017;Çağlar et al 2019).…”

Section: Discussionmentioning

confidence: 99%

First report of 'Candidatus Phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis L.)

Usta¹,

Güller²,

Sipahioğlu³

2021

Plant Prot. Sci.

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The natural occurrence of 'Candidatus Phytoplasma trifolii' in pear trees (Pyrus communis Linnaeus) is reported here for the first time. In 2017, a total of thirty-five pear trees, two of them exhibiting leaf rolling along the midvein, reddening, bushy appearance, and upright growth symptoms were sampled in different locations in Van province, Turkey. The total deoxyribonucleic acid was extracted from symptomatic and asymptomatic plants. The purified DNA served as a template in nested polymerase chain reaction (nested-PCR) assays, performed to amplify 16S rRNA sequences using universal primer pairs (R16mF2/R16mR1 and R16F2n/R16R2). The resulting PCR products were then cloned into a pGEM T-Easy vector and sequenced bidirectionally. The phytoplasma strain, group, and subgroup identity were determined using the in silico restriction fragment length polymorphism (RFLP) analysis of the 16S ribosomal RNA-encoding gene sequences profiling with seventeen distinct restriction enzymes. Of the thirty-five pear samples, only two yielded 1 256 bp and 1 258 bp DNA fragments and were designated as Van-Pr3 (Acc. No. MH709141) and Van-Pr4 (Acc. No. MH730561), respectively. Based on the in silico virtual RFLP pattern analysis of the 16S rRNA sequences, we confirmed the presence of 'Ca. P. trifolii' belonging to the clover proliferation group and both identified phytoplasmas were identical with the similarity coefficient of 1.00 to the reference pattern of 16Sr group VI, subgroup A (Acc. No. AY390261). Here we report that the pear tree is an alternate host of the 'Ca. P. trifolii'.

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Occurrence and identification of grapevine phytoplasmas in main viticultural regions of Turkey

Cited by 16 publications

References 19 publications

Genotyping Points to Divergent Evolution of ‘CandidatusPhytoplasma asteris’ Strains Causing North American Grapevine Yellows and Strains Causing Aster Yellows

Genotyping Points to Divergent Evolution of ‘CandidatusPhytoplasma asteris’ Strains Causing North American Grapevine Yellows and Strains Causing Aster Yellows

Molecular identification of diverse ‘Candidatus Phytoplasma’ species associated with grapevine decline in Iran

First report of 'Candidatus Phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis L.)

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