Occurrence and Inheritance of Esterases in Musca Domestica

Abstract

Esterases in individual houseflies were studied by means of agargel electrophoresis according to WIEME (1959). At least ten different esterases hydrolysing α‐naphthylacetate could be demonstrated to occur in a particular strain of flies. Only one of these was present in all flies studied. A comparison of esterase patterns in parents and offspring showed the technique to be reliable and provided strong evidence that nine different esterase genes are responsible for the occurrence of the ten esterase bands referred to above, one being responsible for the production of two electrophoretically different esterases. Two of these genes were found to be probably allelic. No sex linkage was observed. Zusammenfassung VORKOMMEN UND VERERBUNG VON ESTERASEN BEI DER STUBENFLIEGE, MUSCA DOMESTICA. Mit einer Agargel benutzenden elektrophoretischen Methode nach WIEME wurden die Esterasen einzelner Individuen eines Stubenflicgenstammes studiert Dabei wurden insgesamt 10 verschiedene Esterasen gefunden, die α‐Naphlhylacetat hydrolysieren. Nur eine dieser Esterasen war in allen Fliegen vorhanden. Die Esterase‐Garnituren von Eltern, und Nachkommen wurden verglichen. Allem Anschein nach sind 9 verschiedene Esterase‐Gene für das Auftreten der 10 Esterase‐Banden verantwortlich. Eins der Gene ist für die Erzeugung von 2 elektrophoretisch unterscheidbaren Esterasen zuständig. Eine der anderen. Esterasen wird wahrscheinlich von einem Allel dieses zuletztgenannten Gens bedingt. Geschlechtsgebundenheit wurde nicht festgestellt.

ESTERASE VARIATION AND ORGANOPHOSPHATE RESISTANCE IN POPULATIONS OF APHIS FABAE AND MYZUS PERSICAE

“…Such esterase heterogeneity is known to exist in many animals, including several insects : the fruit fly (Drosophila melanogaster Meigen) (Wright 1961), the milkweed bug (Oncopeltus fasciatus Dall.) (Salkeld 1965), the American cockroach (Periplaneta americana L.) (Cook & Forgash 1965), the honey bee (Apis mellifera L.) (Benton 1967) and the housefly (Musca domestica L.) (Velthuis & van Asperen 1963). Breeding experiments combined with pedigree analysis have demonstrated that this variation is the result of a range of unifactorial genetic differences (Wright 1963;Velthuis & Asperen 1963).…”

“…(Salkeld 1965), the American cockroach (Periplaneta americana L.) (Cook & Forgash 1965), the honey bee (Apis mellifera L.) (Benton 1967) and the housefly (Musca domestica L.) (Velthuis & van Asperen 1963). Breeding experiments combined with pedigree analysis have demonstrated that this variation is the result of a range of unifactorial genetic differences (Wright 1963;Velthuis & Asperen 1963). In A. fabae and M. persicae the sexual cycle is a complex and lengthy process not easily lending itself to breeding experiments (Iglisch 1968;Blackman 1971).…”

Residues of Pesticides and Other Foreign Chemicals in Foods and Feeds / Rückstände von Pesticiden und anderen Fremdstoffen in Nahrungs- und Futtermitteln

“…It was not clear, however, whether the above macromolecular fraction was composed of one or more components. An attempt was made, therefore, to analyze the above DDT-complexes in sodium taurocho- VAN ASPEREN 1963). The agar plate that had been developed through electrophoresis was then sectioned transversely into 25 pieces (usually five mm.…”

GENETIC CONTROL OF MULTIPLE ESTERASES IN <i>MUSCA DOMESTICA</i>

“…From a comparison of the esterase patterns in parents and offspring, Velthuis and van Asperen (1963) suggested that nine different esterase genes are responsible for the occurence of ten esterase bands, and that two of these genes may be allelic. These genes displayed no sex linkage.…”