Occurrence and Microbiological Characteristics of Trichoderma in Al-Jabal Al-Akhdar Region, Libya

Attitalla, Idress Hamad; Abdelrawaf, Suliman S.; Omar, Khawila Saeed; El-Komy, Hesham M. A.; Sarwar, M.

doi:10.3923/jbs.2012.209.217

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…The similar results for isolation of Trichoderma spp. from rhizosphere soils on TSM were found by workers ( [14,9,13,17,3,1,8,11] . Bissett (1984Bissett ( , 1991 a,b, c) [4][5][6][7] classification method.…”

Section: Results and Discussion 31 Sample Collection And Isolationmentioning

confidence: 86%

Survey and isolation of Trichoderma spp. from rhizosphere soils of Western Maharashtra

Wagh¹,

Barhate²

2021

J Pharmacogn Phytochem

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In this study, the total 45 samples were collected from areas of Western Maharashtra. Out of these only 20 rhizospheric soil samples had the population of Trichoderma spp. The Trichoderma was isolated from the soil samples collected from the different locations of western Maharashtra by serial dilution technique and plating method. By visual observations 6, T. hamatum, 7, T harzianum (Rifai), 3, T. koningii (Oudem) and 4, T. asperellum (Samuels) were identified. T. harzianum and T .hamatum were present in most of the soil samples collected from Western Maharashtra region. The T. asperellum species was obtained from soil samples collected from Dhabewadi, sakri, Pachora and Tasgaon region of western Maharashtra. These isolates of Trichoderma spp. were studied and identified on basis of macroscopic i.e. colony characters like growth rates, pigmentation, colony edge, pustule formation showing defined characteristics for that respective species.

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Section: Results and Discussion 31 Sample Collection And Isolationmentioning

confidence: 86%

Survey and isolation of Trichoderma spp. from rhizosphere soils of Western Maharashtra

Wagh¹,

Barhate²

2021

J Pharmacogn Phytochem

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“…The isolation of Trichoderma was performed in the different soil samples in various plant farming models. Attitalla et al (2012) recommended TMS as suitable for the isolation of Trichoderma. TSM is superior compared to Martin's medium (MT), potato dextrose agar (PDA), malt extract medium, glucose-Czapek's agar medium (CZ), and this medium recorded an efficiency ranging from 120%-140%, favouring TSM for further studies on Trichoderma to determine its survival and proliferation in soils.…”

Section: Isolation Of Trichoderma Sppmentioning

confidence: 99%

Potential of Trichoderma spp. isolated in the rhizosphere to produce biofertilizer from organic materials

et al. 2022

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Abstract. Thuy NP, Nam NN, Trai NN, Thao NHX, Phong VT, Khang DT. 2022. Potential of Trichoderma spp. isolated in the rhizosphere to produce biofertilizer from organic materials. Biodiversitas 23: 6386-6396. Trichoderma spp. are filamentous fungi present in nearly all soils and capable of secreting large amounts of cellulase enzymes that can degrade carbohydrate polymer. In the current study, eight isolates of Trichoderma spp. (TC10-RL11, CT11-VO11, CN2-DP11, CN1-DP11, TC9-RL11, CN4-VR11, CK6-VC11, and TC8-RL11) were isolated. Among the eight fungal strains, the CN4-VR11 had highly potent CMC degradation with a halo’s diameter zone of 8.7 ± 1.5 cm. The BLAST result indicates that the CN4-VR11 strains and Trichoderma reesei had a similarity of 98.92% with a max score of 1158 and an E-value of 0.0. The Trichoderma reesei CN4-VR11 was selected for bioproduct production. The application of Trichoderma reesei CN4-VR11 bioproduct passively increased the efficient decomposition of the organic fertilizers, after only 30 days of incubation, the total organic matter content ? 20% was observed to be soft, spongy, and brown-back in color. According to the Government of Vietnam’s Decree No. 108/2017/ND-CP on fertilizer management, dated September 20, 2017, the T5 and T7 treatments were chosen for the production of granular bio-compost, which has a more practical use in agricultural land.

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“…Trichoderma isolation from these soils was performed by the pour plate method using seven media. These media were RB-S-F medium (Davet & Rouxel, 2000), TSM modified (Williams et al, 2003), TME (Vargas et al, 2009), TSM (Attitalla et al, 2012), Davet's medium (Ommati et al, 2013), GRSM (Haddad et al, 2014) and Czapek Yeast Autolysate CYA (Visagie et al, 2014).…”

Section: Collection Of Soil Samples and Isolationmentioning

confidence: 99%

Antifungal Potentiality and Physiological Characterization of Trichoderma Isolates from Port Said Governorate

et al. 2019

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A TOTAL of twenty Trichoderma isolates belonging to seven species were isolated from several locations at Port Said Governorate. Physiological characteristics of these isolates and their antagonistic effect against Sclerotinia sclerotiorum, the causative agent of white mold of Phaseolus vulgaris were evaluated. Trichoderma polysporum 2 showed the highest activity of total cellulase reached up to 2.95 filter paper unit ml-1. T. harzianum 2 had the highest endoglucanase activity (5.47 IU ml-1), whereas T. polysporum 1 had the highest chitinase activity (1.01 IU ml-1). Siderophores production was assayed using FeCl 3 , tetrazolium, Arnow's and spectrophotometric tests. Most of the isolates were able to produce hydroxamate and carboxylate types of siderophores. The ability of Trichoderma to produce indole acetic acid (IAA) and gibberellic acid (GA 3) were also investigated. Trichoderma harzianum 2 showed the highest concentration of IAA in broth medium reached up to 13.19µg/ml, whereas T. polysporum 1 showed the highest concentration of GA 3 by 586.51µg/ml. The antagonistic potentialities of Trichoderma isolates against Sclerotinia sclerotiorum were tested in vitro by using dual culture, base to base assay and the crude liquid extract effect of the isolates. Percentages of inhibition of radial growth of the pathogen by T. polysporum 1 in dual culture and crude extract methods were 45.97% and 87.78%, respectively. Also, the volatiles of T. piluliferum 2 showed a reduction of the radial growth by 66.85%. So, some Trichoderma isolates collected from Port Said soil showed high biocontrol activity against S. sclerotiorum.

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Occurrence and Microbiological Characteristics of Trichoderma in Al-Jabal Al-Akhdar Region, Libya

Cited by 5 publications

References 11 publications

Survey and isolation of Trichoderma spp. from rhizosphere soils of Western Maharashtra

Survey and isolation of Trichoderma spp. from rhizosphere soils of Western Maharashtra

Potential of Trichoderma spp. isolated in the rhizosphere to produce biofertilizer from organic materials

Antifungal Potentiality and Physiological Characterization of Trichoderma Isolates from Port Said Governorate

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