Occurrence and molecular characterization of Staphylococcus aureus strains isolated from meat and dairy products by PCR-RFLP

Abstract

A total of 100 Staphylococcus aureus strains were isolated from 1,047 food samples. In addition to biotyping all isolates, the occurrence of the tst gene and protein were evaluated by PCR and the RPLA test, respectively. Moreover, polymorphism of the X-region of the protein A gene was analyzed by PCR-RFLP. TSST 1 was detected in 12 strains and production of TSST 1 in all strains was confirmed by RPLA assay. It was noteworthy that 66.7% of TSST 1 -producing S. aureus strains belonged to the human biotype. The result of genotyping amplification of the spa gene displayed size polymorphisms and revealed seven different clusters ranging from 1,200 bp to 1,600 bp. Digestion of amplicons with HindIII and HaeII resulted in six distinct patterns for each of them. An amplicon of 1,450 bp in size was the predominant type of Spa-PCR product. The accuracy of the Spa typing system by PCR-RFLP was 100%, thus confirming this method as a reliable tool in routine epidemiological investigation for S. aureus.

Prevalence of Enterotoxigenic Staphylococcus aureus Isolated From Chicken Nugget in Iran

“…According to our results and the results of previous investigations, the sea gene of S. aureus has the highest prevalence of virulence genes. Similar results have been reported by other Iranian authors ( 29 , 30 ). Soltan Dallal et al indicated that from a total of 100 S. aureus strains isolated from 1047 food samples, toxic shock syndrome toxin-1 (TSST1) was detected in 12 strains and 66.7% of them belonged to the human biotype.…”

“…Soltan Dallal et al indicated that from a total of 100 S. aureus strains isolated from 1047 food samples, toxic shock syndrome toxin-1 (TSST1) was detected in 12 strains and 66.7% of them belonged to the human biotype. In addition, they reported that the most prevalent spa ( S. aureus protein A) types of S. aureus isolated from 913 food samples were D (20%) and C (16%) in dairy products and D (6%) and E (3%) in meat products ( 30 ). It seems that the epidemiology and prevalence of S. aureus isolated from poultry nugget samples in Iran is different.…”

Relationship Between Antibiotic Resistance with Spa Gene Polymorphism Coding Protein A and its Typing with PCR-RFLP Technique in S. aureus Isolated from Foodstuffs

“…However, in the study carried out by Soltan Dallal et al there was a significant difference between the frequency of bacteria isolated from dairy products (17%) and meat products (5/3%), (P < 0.05), (16). In contrast with the present study, a research from Italy (2014), the prevalence of S. aureus in milk and dairy products was reported 39% (19), which is higher than our obtained results.…”

“…In addition, this bacterium is one of the 4 most common and important causes of food poisoning (11). Prevalence of S. aureus in this study was 9.3%, which was similar to the study conducted by Soltan Dallal et al from Tehran, which the prevalence of 9.5% has been reported (16). A study carried out by Ali aydin et al from Turkey (2011), stated that the prevalence of S. aureus in foodstuffs was13.8% (17).…”

Total Phenolic and Flavonoid Content and Antibacterial Properties ofPolygonatum orientaleDesf andTiliadasystyla

“…The uncontrolled use of these antimicrobials can increase the drug resistance to antibiotics in most bacteria, which is one of the reasons for the growing use of plants as low-risk, accessible, and cost-effective natural substances for synthetic antibiotics in the treatment of bacterial infections caused by the increasing global studies and the introduction of antibacterial effects of different plants in recent years. Among all the identified substances contained in effective compounds of plants, phenolic compounds or secondary compounds without nitrogen have many biological effects, including antibacterial activity (12)(13)(14).…”