Occurrence and molecular identification of Cryptosporidium species isolated from cattle in Poland

Rzeżutka, Artur; Kaupke, Agnieszka

doi:10.1016/j.vetpar.2013.03.009

Cited by 24 publications

(34 citation statements)

References 26 publications

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“…Cryptosporidium DNA was extracted from 0.1 g (100 μl) of animal feces using a previously described method (Rzeżutka and Kaupke 2013). Extracts containing parasite DNA were used directly for molecular analyses or stored at -20 o C until use.…”

Section: Methodsmentioning

confidence: 99%

“…This allowed an increase in amplification efficiency and a reduction of reaction inhibition. DNA extracts positive at the 18 SSU rRNA gene locus were further investigated using species-specific COWP-PCR assay (Homan et al 1999) with modified temperature profile and reaction conditions (Rzeżutka and Kaupke 2013). All steps of the molecular analysis were controlled by inclusion of the following set of controls: positive and negative extraction control consisting of Cryptosporidium -free pig feces spiked with C. parvum oocysts (Waterborne TM , Inc., New Orleans, LA, USA) at a concentration of 10 4 cells and all reagents containing distillated water instead of the template.…”

Section: Methodsmentioning

confidence: 99%

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Molecular studies on pig cryptosporidiosis in Poland

Rzeżutka¹,

Kaupke²,

Kozyra³

et al. 2014

Polish Journal of Veterinary Sciences

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Cryptosporidium intestinal parasites have been detected in farmed pigs worldwide. Infections are usually asymptomatic with a low number of oocysts shed in pig feces. This makes the recognition of infection difficult or unsuccessful when microscopic methods are used. The aim of this study was molecular identification of Cryptosporidium species in pig herds raised in Poland with regard to the occurrence of zoonotic species. In total, 166 pig fecal samples were tested. The examined pigs were aged 1 to 20 weeks. Overall, 39 pig farms were monitored for parasite presence. The detection and identification of Cryptosporidium DNA was performed on the basis of PCR-RFLP and nucleotide sequence analysis of the amplified 18 SSU rRNA and COWP gene fragments. Infected animals were housed in 21 (53.8%) of the pig farms monitored. The presence of Cryptosporidum was confirmed in 46 (27.7%) samples of pig feces. Among positive fecal samples, 34 (29.3%) were collected from healthy animals, and 12 (24%) from diarrheic pigs. Most infected animals (42.1%) were 2 to 3 months old. The following parasite species were detected: C. scrofarum, C. suis and C. parvum. Indeed, asymptomatic infections caused by C. scrofarum were observed in the majority of the herds. Mixed infections caused by C. suis and C. scrofarum were not common; however, they were observed in 8.6% of the positive animals. C. parvum DNA was found only in one sample collected from a diarrheic pig. The application of molecular diagnostic tools allowed for detection and identification of Cryptosporidium species in pigs. The sporadic findings of C. parvum are subsequent evidence for the contribution of pigs in the transmission of cryptosporidiosis from animals to humans.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular studies on pig cryptosporidiosis in Poland

Rzeżutka¹,

Kaupke²,

Kozyra³

et al. 2014

Polish Journal of Veterinary Sciences

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“…Además, la mayoría de las infecciones son subclínicas y para su reconocimiento se recomienda realizar métodos más sensibles como la reacción en chain reaction (PCR), the current method of choice for diagnosis of the disease. Thus the identification and evaluation of the prevalence of different species of Cryptosporidium has been achieved using molecular tests (5). For this reason, molecular tools have become the key to identification of species (6) and are recognized as essential for the determination of the taxonomy of Cryptosporidium.…”

Section: Diagnóstico Molecularmentioning

confidence: 99%

“…cadena de la polimerasa (PCR), método de elección al momento del diagnóstico de la enfermedad; por lo tanto, la identificación y evaluación de la prevalencia de las diferentes especies de Cryptosporidium spp. han sido realizada a través de pruebas moleculares (5). Por esta razón, las herramientas moleculares se han convertido en la clave para la identificación de especies (6) y son reconocidas como esenciales para determinar la taxonomía de Cryptosporidium, favoreciendo el entendimiento de la biología, epidemiología y salud relacionando la importancia de varias especies de Cryptosporidium y aquellas poblaciones conocidas como genotipos que no han sido reconocidos como especie (1).…”

Section: Diagnóstico Molecularunclassified

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Caracterización molecular de los subtipos de la GP60 de Cryptosporidium parvum y Cryptosporidium hominis alrededor del mundo

Avendaño

Amaya

2017

Rev MVZ Córdoba

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Cryptosporidium is a zoonotic parasite very important in animal health as well as in public health. It is because this is one of the main causes of diarrhea in children, calves, lambs and other variety of youth mammalians in a lot of countries. The globalization has enabled the exchange of biological material in different regions worldwide, encouraging the spread of diseases and exposure to these biological agents to different environmental conditions, inducing adaptation through genetic changes. Based in the polymorphism of the gene for GP60, this review intended to present the distribution of Cryptosporidium parvum and Cryptosporidium hominis in humans and calves worldwide. The subtype that affects cattle more frequently corresponds to IIaA15G2R; while the subtype most frequently isolated from human samples is IaA19G2.Keywords: Cryptosporidiosis, molecular epidemiology, public health (Source: MeSH). REsUMENCryptosporidium es un parásito zoonótico muy importante en salud animal así como en salud pública. Esto se debe a que el parásito se constituye en una de las principales causas de diarrea en niños, terneros, corderos y una gran variedad de mamíferos jóvenes en una gran cantidad de países. Debido a que la globalización ha permitido el intercambio de material biológico en diferentes regiones alrededor del mundo, se ha favorecido la propagación de enfermedades y se han expuesto a los agentes biológicos a diferentes condiciones ambientales, induciendo así la adaptación a través de cambios genéticos. Con base en el polimorfismo del gen GP60, esta revisión pretende presentar la distribución de Cryptosporidium parvum y Cryptosporidium hominis en humanos y terneros alrededor del mundo. El subtipo que afecta con mayor frecuencia al ganado vacuno corresponde a IIaA15G2R1; en tanto que el subtipo aislado con mayor frecuencia a partir de las muestras humanas es IaA19G2.Palabras clave: Criptosporidiosis, epidemiología molecular, salud pública (Fuente: MeSH).

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Emergence of novel subtypes of Cryptosporidium parvum in calves in Poland

Kaupke

Rzeżutka

2015

Parasitol Res

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The aim of the study was to identify the Cryptosporidium parvum subtypes circulating in Polish cattle and their distribution in relation to the age and health status of tested animals. In total, 779 fecal samples were obtained from young cattle originating from 237 farms. C. parvum strains were identified at the 18 small-subunit ribosomal RNA (SSU rRNA), COWP, and LIB13 loci and were subsequently analyzed by sequencing at the 60-kDa glycoprotein (GP60) locus for subtype determination. The presence of 71 C. parvum strains belonging to IIa, IId, or IIl subtype families was shown. The strains from the IIa allele family prevailed with IIaA17G1R1, IIaA17G2R1, and IIaA15G2R1 subtypes occurring frequently. Two novel subtypes IIaA10G1R1 and IIlA19R3 were detected for the first time in a bovine host. The highest C. parvum prevalence (22.5 %, confidence interval (CI) = 2.5 %) was observed among the youngest animals up to 2 weeks of age, followed by the prevalence among those aged 2 to 4 weeks (6.6 %, CI = 2.6 %) and then among older cattle (4.9 %, CI = 2.1). The occurrence of diarrhea in animals was associated with the presence of the IIaA16G1R1b subtype, while infections caused by IIaA15G2R1 strains were more likely to be asymptomatic. The geographical distribution of subtypes revealed that strains from the IIa subtype family were detected all over the country frequently compared to the IId and IIl subtypes, the sporadic appearances of which confirmed their endemic occurrence. Subtype analysis revealed the presence of zoonotic strains indicating cattle as a reservoir for human cryptosporidiosis.

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Occurrence and molecular identification of Cryptosporidium species isolated from cattle in Poland

Cited by 24 publications

References 26 publications

Molecular studies on pig cryptosporidiosis in Poland

Molecular studies on pig cryptosporidiosis in Poland

Caracterización molecular de los subtipos de la GP60 de Cryptosporidium parvum y Cryptosporidium hominis alrededor del mundo

Emergence of novel subtypes of Cryptosporidium parvum in calves in Poland

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