Occurrence and persistence of Bacillus thuringiensis (Bt) and transgenic Bt corn cry1Ab gene from an aquatic environment

Douville, Mélanie; Gagné, François; Blaise, C.; André, C.

doi:10.1016/j.ecoenv.2006.01.002

Cited by 82 publications

(62 citation statements)

References 25 publications

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“…The aquatic component of nontarget organisms has received little attention. However, transgenic cry1Ab genes have recently been shown to drain, persist, and possibly be transported long distances into aquatic freshwater ecosystems from agricultural fields with GM Bt-maize (Douville et al 2007). Douville and colleagues concluded that the cry1Ab transgene is likely to be expressed in aquatic environments and recommended further monitoring strategies to characterize environmental exposure and effects.…”

Section: Introductionmentioning

confidence: 99%

Reduced Fitness of Daphnia magna Fed a Bt-Transgenic Maize Variety

Bøhn

Primicerio

Hessen

et al. 2008

Arch Environ Contam Toxicol

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Genetically modified (GM) maize expressing the Bt-toxin Cry1Ab (Bt-maize) was tested for effects on survival, growth, and reproduction of the water flea Daphnia magna, a crustacean arthropod commonly used as a model organism in ecotoxicological studies. In three repeated experiments, D. magna were fed 100% ground maize in suspension, using either GM or isogenic unmodified (UM) maize. D. magna fed GM-maize showed a significantly reduced fitness performance: The mortality was higher, a lower proportion of females reached sexual maturation, and the overall egg production was lower compared to D. magna fed UM isogenic maize. We conclude that the tested variety of Bt-maize and its UM counterpart do not have the same quality as food sources for this widely used model organism. The combination of a reduced fitness performance combined with earlier onset of reproduction of D. magna fed Bt-maize indicates a toxic effect rather than a lower nutritional value of the GMmaize.

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Section: Introductionmentioning

confidence: 99%

Reduced Fitness of Daphnia magna Fed a Bt-Transgenic Maize Variety

Bøhn

Primicerio

Hessen

et al. 2008

Arch Environ Contam Toxicol

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“…We provide evidence suggesting that CryIAb protein and DNA may arise from cells of B. thuringiensis already present in soils, even though we can not completely exclude the plant origin of the gene in soils with Bt and non Bt maize, as reported recently by Douville et al (2007), using quantitative real time PCR.…”

Section: Discussionmentioning

confidence: 51%

“…The yield of DNA extracted from soil samples was low according to results previously reported (Douville et al 2007). An average of 0.8 µg/g soil was fluorometrically quantified.…”

Section: Resultsmentioning

confidence: 99%

Bt protein rhizosecreted from transgenic maize does not accumulate in soil

Margarit¹,

Reggiardo²,

Permingeat³

2008

Electron. J. Biotechnol.

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“…The release of transgenic plants in a soil environment may increase the risk of contamination by transgenic DNA. Douville et al (2007) reported that the exogenous gene from Bt maize was found in nearby streams and rivers, occasionally even several kilometers downstream, indicating contamination by agricultural transgenic DNA. Some studies have detected the recombinant DNA of transgenic plants in soil bacteria, although some controlled studies have demonstrated natural transformation (De-Vries et al, 2004).…”

Section: Horizontal Transfer Of the Target And Marker Genesmentioning

confidence: 99%

Effect of the Leaf Litter of Transgenic <i>Populus Simonii P. nigra</i> on the Soil Microbial Community and Horizontal Transfer Possibility of the Foreign Gene

Peng¹,

Shah²,

Wang³

et al. 2017

mmr

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To study the potential influence of transgenic Populus simonii×P. nigra on the soil microbial community composition and horizontal exogenous gene transfer, fresh transgenic leaves were treated in broadleaf and coniferous forest soil for 6 months in the laboratory. The results showed that DNA fragments of the target and marker genes were completely degraded before 120 days. The dominant colony is the bacterium whose number is much more than that of other microbes under the same treatment. The number of cultivable bacteria treated by transgenic litters was significantly higher than the number treated by non-transgenic litters in selective medium with kanamycin. The target gene in the entophytic and soil bacteria, with 1.92% and 3.08% PCR-positive results, was found only in broadleaf forest soil. This result showed a certain possibility of horizontal gene transfer from the transgenic poplar to soil bacteria.

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Occurrence and persistence of Bacillus thuringiensis (Bt) and transgenic Bt corn cry1Ab gene from an aquatic environment

Cited by 82 publications

References 25 publications

Reduced Fitness of Daphnia magna Fed a Bt-Transgenic Maize Variety

Reduced Fitness of Daphnia magna Fed a Bt-Transgenic Maize Variety

Bt protein rhizosecreted from transgenic maize does not accumulate in soil

Effect of the Leaf Litter of Transgenic <i>Populus Simonii P. nigra</i> on the Soil Microbial Community and Horizontal Transfer Possibility of the Foreign Gene

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