Occurrence and Probabilistic Risk Assessment of Fumonisin B1, Fumonisin B2 and Deoxynivalenol in Nixtamalized Maize in Mexico City

Gilbert-Sandoval, Ixchel; Wesseling, Sebastiaan; Rietjens, I.M.C.M.

doi:10.3390/toxins12100644

Cited by 14 publications

(4 citation statements)

References 29 publications

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“…Alternatively, the QuEChERS method, initially developed for pesticides, was subsequently introduced for the dispersive solid-phase extraction (SPE) of FB1 [46], and further validated for its application in the multi-target analysis due to its lower cost, less time consumption, easy procedure [50,52,63,185,186], as well as its availability in extraction kits [54] with satisfactory recoveries [60]. Likewise, sample preparation with a QuEChERS dispersive SPE was useful for minimization of matrix effects from beer, with a preconcentration step producing enhanced LODs [74].…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida¹,

Gong²,

Goycoolea³

2020

Preprint

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Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

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Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida¹,

Gong²,

Goycoolea³

2020

Preprint

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“…Furthermore, total fumonisin levels ranged from 0.28 to 15 mg/kg with a mean of 1.54 ± 0.12 mg/kg, and 20% of the prepared feed samples randomly collected from 133 commercial poultry farms, 76 feed processors and 8 feed vendors of Ghana were higher than the FAO/WHO maximum tolerable daily intake limit of 2 mg/kg [11]. Studies also revealed that 47% of Mexican men and 30% of Mexican women might exceed the provisional tolerable daily intake (PMTDI) of 2 µg/kg bw/day Toxins 2022, 14, 0 2 of 12 for FBs [12]. Aspergillus niger, intended for use in the food industry in China, can cause dynamic FB2 production to reduce human exposure to FBs [13].…”

Section: Introductionmentioning

confidence: 98%

“…Toxins 2022, 14, x FOR PEER REVIEW 2 of 12 women might exceed the provisional tolerable daily intake (PMTDI) of 2 μg/kg bw/day for FBs [12]. Aspergillus niger, intended for use in the food industry in China, can cause dynamic FB2 production to reduce human exposure to FBs [13].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Fumonisins by the Consecutive Action of a Fusion Enzyme

et al. 2022

Toxins

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Fumonisins (FBs) are toxic mycotoxins that commonly exist in food and feed. FBs can induce many aspects of toxicity, leading to adverse effects on human and animal health; therefore, investigating methods to reduce fumonisin contamination is necessary. In our study, we generated a recombinant fusion enzyme called FUMDI by linking the carboxylesterase gene (fumD) and the aminotransferase gene (fumI) by overlapping polymerase chain reaction (PCR). The fusion enzyme FUMDI was successfully, secretively expressed in the host Pichia pastoris (P. pastoris) GS115, and its expression was optimized. Our results demonstrated that the fusion enzyme FUMDI had high biodegradation activity of fumonisin B1 (FB1) and other common FBs, such as fumonisin B2 (FB2) and fumonisin B3 (FB3), and almost completely degraded 5 μg/mL of each toxin within 24 h. We also found that FUMDI enzyme and its reaction products had no negative effect on cell viability and did not induce cell apoptosis, oxidative stress, or endoplasmic reticulum (ER) stress in a human gastric epithelial cell line (GES-1). The results indicated that these FBs degradation products cannot have adverse effects in a cell model. In conclusion, a safe and efficient fumonisin-degrading enzyme was discovered, which could be a new a technical method for hazard control of FBs in the future.

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“…The EDI comprises a combination of food consumption data, typical contamination levels, and consumer body weight. Although previous surveys have shown that FB exposure levels in many countries, including Tanzania, Somalia, Mexico, and Brazil, exceeded the PMTDI [ 24 , 25 , 26 , 27 ], indicating potential health risks from dietary exposure to different food products, few studies have assessed the potential human health risks of consuming PFT contaminated with FBs. To the best of our knowledge, the risk assessment of FBs in PFT in Guangxi has not been previously reported.…”

Section: Introductionmentioning

confidence: 99%

B-Type Fumonisins in Post-Fermented Tea: Occurrence and Consumer Dietary Exposure in Guangxi, China

Qiu,

Zhu,

Zhang

et al. 2023

Toxins

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Post-fermented tea (PFT), a commonly consumed beverage worldwide, is characterized by the rapid growth of its microbial groups and the substantial changes they undergo. Consequently, PFT may contain mycotoxins such as B-type fumonisins (FBs). This study aimed to assess the intake of FBs through the consumption of PFT among consumers in Guangxi, China. A novel quantitative method using high-performance liquid chromatography-mass spectrometry was used to determine the FB concentration in PFT products. Additionally, a PFT consumption survey was conducted using a face-to-face questionnaire, recording their body weight and PFT consumption patterns based on a three-day dietary recall method. Finally, hazard index was calculated to estimate the health risk of FBs from the consumption of PFT products in Guangxi. The results revealed that the occurrence of FBs in PFT was 20% (24/120), with a concentration ranging from 2.14 to 18.28 μg/kg. The results of the survey showed that the average daily consumption of PFT by consumers was 9.19 ± 11.14 g. The deterministic risk assessment revealed that only 0.026% of the provisional maximum tolerable daily intake of FBs was consumed through PFT, indicating that FB contamination in PFT is not a public health risk.

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Occurrence and Probabilistic Risk Assessment of Fumonisin B1, Fumonisin B2 and Deoxynivalenol in Nixtamalized Maize in Mexico City

Cited by 14 publications

References 29 publications

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Biodegradation of Fumonisins by the Consecutive Action of a Fusion Enzyme

B-Type Fumonisins in Post-Fermented Tea: Occurrence and Consumer Dietary Exposure in Guangxi, China

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