Occurrence and purification of .alpha.-amylase isoinhibitors in bean (Phaseolus vulgaris L.) varieties

Iguti, Antonia Miwa; Lajolo, Franco Maria

doi:10.1021/jf00012a006

Cited by 17 publications

(8 citation statements)

References 14 publications

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“…Our findings indicate that the bean genome harbors more than one eAI gene, just as it has more than one lectin gene. This confirms biochemical work that many cultivars contain two distinct forms of the inhibitor [14]. Amino acid sequencing of eAIs purified from seed meal of P. vulgaris cv.…”

Section: Discussionsupporting

confidence: 81%

Evolutionary relationships among proteins in the phytohemagglutinin-arcelin-?-amylase inhibitor family of the common bean and its relatives

et al. 1994

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The common bean, Phaseolus vulgaris, contains a family of defense proteins that comprises phytohemagglutinin (PHA), arcelin, and alpha-amylase inhibitor (alpha AI). Here we report eight new derived amino acid sequences of genes in this family obtained with either the polymerase chain reaction using genomic DNA, or by screening cDNA libraries made with RNA from developing beans. These new sequences are: two alpha AI sequences and arcelin-4 obtained from a wild accession of P. vulgaris that is resistant to the Mexican bean weevil (Zabrotes subfasciatus) and the bean weevil (Acanthoscelides obtectus); an alpha AI sequence from the related species P. acutifolius (tepary bean); a PHA and an arcelin-like sequence from P. acutifolius; an alpha AI-like sequence from P. maculatus; and a PHA sequence from an arcelin-5 type P. vulgaris. A dendrogram of 16 sequences shows that they fall into the three identified groups: phytohemagglutinins, arcelins and alpha AIs. A comparison of these derived amino acid sequences indicates that one of the four amino acid residues that is conserved in all legume lectins and is required for carbohydrate binding is absent from all the arcelins; two of the four conserved residues needed for carbohydrate binding are missing from all the alpha AIs. Proteolytic processing at an Asn-Ser site is required for the activation of alpha AI, and this site is present in all alpha AI-like sequences; this processing site is also found at the same position in certain arcelins, which are not proteolytically processed. The presence of this site is therefore not sufficient for processing to occur.

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Section: Discussionsupporting

confidence: 81%

Evolutionary relationships among proteins in the phytohemagglutinin-arcelin-?-amylase inhibitor family of the common bean and its relatives

et al. 1994

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“…This is in agreement with previous studies in which microwave treatment increased the α ‐amylase activity in wheat seedlings and increased the amylase inhibitor level in cowpeas by 50% . This increase has been partly attributed to the presence of heat‐stable amylase inhibitors similar to those found in beans . However, our results contrast with the expected increase in α ‐amylase activity of micronized beans or the reduction in α ‐amylase activity of microwave‐ and infrared‐treated raw and soaked sorghum …”

Section: Resultssupporting

confidence: 93%

Microwave and micronization treatments affect dehulling characteristics and bioactive contents of dry beans (Phaseolus vulgaris L.)

Oomah

Kotzeva

Allen³

et al. 2013

J. Sci. Food Agric.

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“…These are typical lectines, synthesised in rough endoplasmic reticulum and modified through Golgi apparatus in the form of signal peptide removal and N-glycosylation. They possess homologous amino acid sequences, from 50 to 90% [ 18 , 19 ].…”

Section: Bioactive Substances Contained In Leguminous Plantsmentioning

confidence: 99%

The Active Role of Leguminous Plant Components in Type 2 Diabetes

Gętek

Czech

Muc-Wierzgoń

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Diabetes appears to be one of the most frequent noncommunicable diseases in the world. A permanent growth in the incidence of diabetes can be observed and according to the International Diabetes Federation (IDF) the year 2030 will mark the increase in the number of diabetics to 439 mln worldwide. Type 2 diabetes accounts for about 90% of all diabetes incidence. Nutrition model modification not only features the basic element in type 2 diabetes treatment but also constitutes the fundamental factor influencing a morbidity rate decrease. Leguminous plants are a key factor in the diabetic diet; plants such as pulses or soybeans are nutritious products valued highly in nutrition. These legumes are high in the content of wholesome protein and contain large amounts of soluble alimentary fiber fractions, polyunsaturated fatty acids, vitamins and minerals, and bioactive substances with antioxidant, anti-inflammatory, and anticancer activity. They are distinguished by the high amount of bioactive compounds that may interfere with the metabolism of glucose. The most significant bioactive compounds displaying antidiabetic activity in leguminous plants are as follows: genistein and daidzein, alpha-amylase inhibitors, and alpha-glucosidase inhibitors. In vitro research using leguminous plant extracts has confirmed their antidiabetic properties. Leguminous plants should be employed in the promotion of healthy lifestyles in terms of functional food.

show abstract

Occurrence and purification of .alpha.-amylase isoinhibitors in bean (Phaseolus vulgaris L.) varieties

Cited by 17 publications

References 14 publications

Evolutionary relationships among proteins in the phytohemagglutinin-arcelin-?-amylase inhibitor family of the common bean and its relatives

Evolutionary relationships among proteins in the phytohemagglutinin-arcelin-?-amylase inhibitor family of the common bean and its relatives

Microwave and micronization treatments affect dehulling characteristics and bioactive contents of dry beans (Phaseolus vulgaris L.)

The Active Role of Leguminous Plant Components in Type 2 Diabetes

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