Occurrence and survival of potato scab pathogens (<i>Streptomyces</i> species) on tuber lesions: quick diagnosis based on a PCR‐based assay

Lehtonen, Mari; Rantala, Heikki; Kreuze, Jan; Bång, Hans; Kuisma, Leena; Koski, Pertti; Virtanen, Elina; Vihlman, K.; Valkonen, Jari P. T.

doi:10.1111/j.0032-0862.2004.01009.x

Cited by 67 publications

(94 citation statements)

References 22 publications

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“…Many locations harbored more than one species, and there were isolates from a single lesion belonging to two species. Valkonen's group in Finland has also reported that more than one species can co-inhabit single CS lesions (Lehtonen et al 2004). From these observations, we can conclusively say that pathogenic Streptomyces in single fields and even single lesions are not clonal.…”

Section: Discussionmentioning

confidence: 64%

A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

Wanner

2009

Am. J. Pot Res

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To determine distribution and prevalence of Streptomyces species associated with common scab in North America, more than 1,400 isolates were made from scabby potato tubers. Samples were obtained from locations across the continent, and from multiple sites in close geographic proximity. Most potentially pathogenic isolates belonged to four species previously associated with common scab, S. scabies, S. europaeiscabiei, S. stelliscabiei, and S. sp. IdahoX. Additionally, small numbers of potentially pathogenic isolates were identified as S. acidiscabies, S. turgidiscabies and six additional ribotypes. S. europaeiscabiei was most common in the west and S. scabies predominated in the middle and eastern Midwest. Beyond this geographic trend, species distribution was patchy. A single species often predominated in a field while neighboring fields had different species. Local and regional variation was seen when isolates were genotyped for repetitive sequence element patterns, reflecting the conserved or core genome, and genes characteristic of the Streptomyces pathogenicity island, reflecting flexible components of the Streptomyces genome. This is the largest survey to date of the distribution of common scab-causing species, and will be a resource for population biology studies and for tracing the evolutionary history and spread of common scabassociated Streptomyces.Resumen Para determinar la distribución y prevalencia de especies de Streptomyces asociadas con la sarna común en Norteamérica, se hicieron más de 1,400 aislamientos de tubérculos de papa con sarna. Las muestras fueron obtenidas de localidades de todo el continente y de múltiples lugares geográficamente próximos. Los aislamientos potencialmente más patogénicos fueron de cuatro especies previamente asociadas con sarna común, S. scabies, S. europaeiscabiei, S. stellicabiei y S. sp. IdahoX. Adicionalmente, un número pequeño de aislamientos potencialmente patogénicos fueron identificados como S. acidiscabies, S. turgidiscabies y seis ribotipos adicionales. S. europaeiscabiei fue el más común en el oeste y S. scabies predominó en el centro y este del Medio Oeste. Más allá de esta tendencia geográfica, la distribución de especies fue en parches. Una sola especie a menudo predominó en un campo mientras en los campos vecinos se encontraron diferentes especies. Se vieron variaciones locales y regionales cuando a los aislamientos se les determinó el genotipo para patrones de elementos con secuencias repetitivas que reflejan el genoma conservado o central, y genes característicos de la isla de patogenicidad de Streptomyces, reflejando componentes flexibles del genoma de Streptomyces. Este es la pesquisa más extensa a la fecha sobre la distribución de las especies que causan sarna común, y será un recurso para estudios de biología poblacional y para determinar la historia evolutiva y diseminación de Streptomyces asociado a la sarna común.Keywords Streptomyces scabies . Population genetics . Pathogenicity island Abbreviations CSCommon scab PAI Pathogenicity islan...

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Section: Discussionmentioning

confidence: 64%

A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

Wanner

2009

Am. J. Pot Res

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“…This is contrary to what we found; tubers showing netted scab symptoms mostly originated from the Western Cape which includes the Sandveld region, known for its sandy soils. It is possible that the lack of a specific geographical species distribution could be due to the use of infected seed tubers or that more than one species could naturally occur in a specific location (Lehtonen et al 2004;Wanner 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Washing tubers and even storing tubers before isolation of the pathogen can result in more S. turgidiscabies than S. scabiei being isolated from the lesions (Lehtonen et al 2004;Valkonen 2004). Dees et al (2013) could only find S. turgidiscabies in a few countries but attributed this to the small sample size.…”

Section: Discussionmentioning

confidence: 99%

“…These are not the only scab-causing species and reports of other species are geographically distinct. S. scabiei is found worldwide but is not common in Europe; while S. europaeiscabiei is more often found in Europe, Korea and North America; and S. turgidiscabies is frequently found in Japan and Finland (Lehtonen et al 2004;Wanner 2009;. A multitude of other, poorly described Streptomyces spp.…”

Section: Introductionmentioning

confidence: 99%

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Streptomyces species associated with common scab lesions of potatoes in South Africa

Jordaan

Waals

2015

Eur J Plant Pathol

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Streptomyces scabiei is largely accepted as the causal organism of common scab on potato in South Africa, and other Streptomyces species associated with common scab are not often considered. This study therefore aims to determine the diversity and prevalence of Streptomycetes associated with common scab on potatoes in South Africa.Isolates from 11 of the 16 potato producing regions in South Africa were characterized morphologically, physiologically and genetically. Most isolates resembled S. scabiei based on morphology and physiology. Most pathogenic isolates were S. scabiei and S. stelliscabiei, and no S. acidiscabies or S. turgidiscabies isolates were found. All three pathogenicity / virulence genes (txtAB, nec1, tomA) were found in South African isolates.Pathogenicity could not be linked to the presence of a single one or any combination of two of the three genes.These results represent the most comprehensive published survey of Streptomycetes isolated from common scab lesions on potatoes in South Africa.

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“…These are important study areas related to plant biotechnology carried out at DAB (Lehtonen et al 2004, 2008a, Mattinen et al 2004, Aittamaa et al 2008, Hiltunen et al 2008, other departments of AF-UH and the Viikki Campus (e.g., Pitkäjärvi et al 2003, Jussila et al 2006, Sjöblom et al 2006. At DAB, these studies are carried out on many crop plant species and their pathogens and also on the model species Arabidopsis thaliana (Karim et al 2007) and the moss, Physcomitrella patens, to understand evolutionary early-developed mechanisms of pathogen defence in plants (Akita and Valkonen 2002, Andersson et al 2005, Lehtonen et al 2006).…”

Section: Molecular Markers and Their Multiple Usesmentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

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Plants bind solar energy to organic matter via photosynthesis and assimilation of carbon dioxide from the atmosphere and comprise the major source of nutrition and bioenergy. Plant biotechnology contributes to solution of important constraints in food and feed production and creates new technologies and applications for the sustainable use of plant resources. Genome-wide approaches such as massive parallel sequencing and microarrays to study gene expression, molecular markers for selection of important traits in breeding, characterization of genetic diversity with the aforementioned approaches, and somatic hybridization and genetic transformation are important tools in plant biotechnology. In this paper, studies carried out on enhanced resistance to viruses and tolerance of cold stress in potato, genetic modification of flower pigmentation and morphology in gerbera, production of edible vaccines in transgenic barley seeds, and expression of heterologous proteins for pharmaceutical purposes from vector viruses were chosen to exemplify the general utility of biotechnological approaches and also how plant biotechnology research has developed on cultivated plants at University of Helsinki. The studies reveal cellular and genetic mechanisms and provide scientific information that can be used for widening the uses of crop plants. They can also be used to detect any putative risks associated with the use of the biotechnological application in agriculture and horticulture and to develop practises which reduce any inadvertent negative consequences that plant production may have to the environment.

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Occurrence and survival of potato scab pathogens (Streptomyces species) on tuber lesions: quick diagnosis based on a PCR‐based assay

Cited by 67 publications

References 22 publications

A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

Streptomyces species associated with common scab lesions of potatoes in South Africa

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

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