Occurrence and typing of Listeria monocytogenes strains in retail vacuum-packed fish products and in a production plant

Johansson, Tuula; Rantala, Leila; Palmu, Liisa; Honkanen-Buzalski, T.

doi:10.1016/s0168-1605(99)00019-7

Cited by 133 publications

(73 citation statements)

References 21 publications

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“…Raw fish was shown not to be an important source for L. monocytogenes contamination of the smoked salmon (20). Johansson and coworkers (11) found one PFGE type persisting in a smokehouse for 14 months. Autio and collaborators (1) found that in one rainbow trout smokehouse there were two major contamination sites, the brining and slicing areas, which seemed to be colonized by different pulsotypes.…”

Section: Discussionmentioning

confidence: 99%

“…This is consistent with what was reported from Sweden, where human isolates during the 1990s were equally distributed between serotypes 1/2 and 4. In Finland, serotype 1/2 has been recently more frequently isolated from patients (11), and in other countries also sero- type 1 seems to have been increasing in the last several years (14). Some ETs (ET-1, ET-6, and ET-11) were identified from several ready-to-eat foods including smoked salmon, as well as from raw fish.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Epidemiological Survey of Listeria monocytogenes in Seafoods and Seafood-Processing Plants

Rørvik

Aase

Alvestad³

et al. 2000

Appl Environ Microbiol

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To evaluate the role of seafoods in the epidemiology of human listeriosis and the role of the processing environment as a source of Listeria monocytogenes in seafood products, 305 L. monocytogenes isolates were characterized by multilocus enzyme electrophoresis using 21 genetic loci and restriction enzyme analysis of total DNA. Forty-four isolates were recovered from patients in Norway; 93 were isolated from seafoods, seafood-processing environments, and seawater from 55 different producers; and the remaining 168 isolates originated from six seafood-processing plants and one transport terminal examined in detail for L. monocytogenes. The patient isolates fell into 11 electrophoretic types, with four of them being responsible for 77% of the listeriosis cases in 1992 to 1996. Isolates from Norwegian seafoods and processing environments showed great genetic diversity, indicating that seafoods and seafood-processing environments do not offer a niche for specific L. monocytogenes strains. On the other hand, isolates from individual processing plants were genetically more homogenous, showing that plants are likely to be colonized with specific subclones of L. monocytogenes. The isolation of identical subclones of L. monocytogenes from both human patients and seafoods, including ready-to-eat products, suggests that such products may have been possible sources for listeriosis cases in Norway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiological Survey of Listeria monocytogenes in Seafoods and Seafood-Processing Plants

Rørvik

Aase

Alvestad³

et al. 2000

Appl Environ Microbiol

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“…Clinically, symptoms of listeriosis include meningitis, septicemia, spontaneous abortion, perinatal infections, and gastroenteritis (25). Listeria species are found throughout the food-processing environment (9,17,28,36) and can survive adverse conditions such as high salt levels and both pH (32) and temperature (48) extremes. However, the organism does not survive thermal processing; contamination of food generally results from contact of a processed food with a spoiled surface prior to packaging (27) or through the use of contaminated ingredients in minimally processed foods.…”

mentioning

confidence: 99%

Flagellin fromListeria monocytogenesIs Glycosylated with β-O-LinkedN-Acetylglucosamine

et al. 2004

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“…While some studies have pointed to raw materials as the most likely sources of product contamination with L. monocytogenes (7,15), other studies have found that the major source of contamination is the processing environment and equipment (2,21,30,40).…”

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confidence: 99%

Elucidation of Listeria monocytogenes Contamination Routes in Cold-Smoked Salmon Processing Plants Detected by DNA-Based Typing Methods

Vogel

Huss

Ojeniyi

et al. 2001

Appl Environ Microbiol

213

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The contamination routes of Listeria monocytogenes in cold-smoked salmon processing plants were investigated by analyzing 3,585 samples from products (produced in 1995, 1996, 1998, and 1999) and processing environments (samples obtained in 1998 and 1999) of two Danish smokehouses. The level of product contamination in plant I varied from 31 to 85%, and no L. monocytogenes was found on raw fish (30 fish were sampled). In plant II, the levels of both raw fish and product contamination varied from 0 to 25% (16 of 185 raw fish samples and 59 of 1,000 product samples were positive for L. monocytogenes). A total of 429 strains of L. monocytogenes were subsequently compared by random amplified polymorphic DNA (RAPD) profiling, and 55 different RAPD types were found. The RAPD types detected on the products were identical to types found on the processing equipment and in the processing environment, suggesting that contamination of the final product (cold-smoked salmon) in both plants (but primarily in plant I) was due to contamination during processing rather than to contamination from raw fish. However, the possibility that raw fish was an important source of contamination of the processing equipment and environment could not be excluded. Contamination of the product occurred in specific areas (the brining and slicing areas). In plant I, the same RAPD type (RAPD type 12) was found over a 4-year period, indicating that an established in-house flora persisted and was not eliminated by routine hygienic procedures. In plant II, where the prevalence of L. monocytogenes was much lower, no RAPD type persisted over long periods of time, and several different L. monocytogenes RAPD types were isolated. This indicates that persistent strains may be avoided by rigorous cleaning and sanitation; however, due to the ubiquitous nature of the organism, sporadic contamination occurred. A subset of strains was also typed by using pulsed-field gel electrophoresis and amplified fragment length polymorphism profiling, and these methods confirmed the type division obtained by RAPD profiling.

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Occurrence and typing of Listeria monocytogenes strains in retail vacuum-packed fish products and in a production plant

Cited by 133 publications

References 21 publications

Molecular Epidemiological Survey of Listeria monocytogenes in Seafoods and Seafood-Processing Plants

Molecular Epidemiological Survey of Listeria monocytogenes in Seafoods and Seafood-Processing Plants

Flagellin fromListeria monocytogenesIs Glycosylated with β-O-LinkedN-Acetylglucosamine

Elucidation of Listeria monocytogenes Contamination Routes in Cold-Smoked Salmon Processing Plants Detected by DNA-Based Typing Methods

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