Occurrence, Antibiotic Resistance, Virulence Factors, and Genetic Diversity of <i>Bacillus</i> spp. from Public Hospital Environments in South Africa

Mbhele, Zamile N.; Shobo, Christiana O.; Amoako, Daniel G.; Zishiri, Oliver T.; Bester, Linda A.

doi:10.1089/mdr.2020.0543

Cited by 9 publications

(10 citation statements)

References 58 publications

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“… 9 However, our prevalence was higher than the rate of 17% reported previously in KwaZulu-Natal province, South Africa. 2 In this study, the prevalence of B. cereus (46.6%) was higher than prevalence rate (16%) in a hospital setting reported previously in the St. Azzhria University Hospital in Isfahan, Malaysia. 11…”

Section: Discussioncontrasting

confidence: 62%

“… 1 Bacillus infections have been recorded sporadically in surgical wounds, eye infections, pneumonia, bacteremia, meningitis, sepsis and soft tissue infections, but particularly in immuno-compromised individuals. 2 Infections acquired in a hospital setting significantly increase morbidity and mortality rates, lengthen hospital stays and raise health care expenses. 3…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and prevalence of Bacillus species isolated from Saudi hospitals

Bakri

2023

Journal of Taibah University Medical Sciences

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Section: Discussioncontrasting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Molecular characterization and prevalence of Bacillus species isolated from Saudi hospitals

Bakri

2023

Journal of Taibah University Medical Sciences

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“…This underscores the call by some researchers for the design of optimal strategies to curb the spread of Bacillus spp. in hospital settings [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Contamination of Surgical Instruments Used at the Surgery Department of a Major Teaching Hospital in a Resource-Limited Country: An Observational Study

et al. 2022

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Surgical instruments, be they disposable or reusable, are essential in any surgical procedure. Reusable surgical instruments should be properly sterilized or disinfected before use. However, the protocols are not always followed accordingly. This results in sterilization and disinfection failures, leading to a possible increase in the incidence of surgical site infections. This observational study report on bacterial contaminants identified instruments used for surgical procedures in a major teaching hospital in a resource-limited country. In total, 207 pre-sterilized surgical instruments and instrument parts used at three units—the general surgical theater, and the gastrointestinal (GI) endoscopy and urology endoscopy (uro-endoscopy) units—within the surgical department were randomly sampled and examined for bacterial contamination. Bacteria isolates were identified, and their antimicrobial susceptibility patterns were determined. Bacteria isolates that were identified included Citrobacter spp., Citrobacter freundii, Bacillus cereus, Staphylococcus hominis, and Staphylococcus aureus. Bacillus cereus was the most predominant bacteria isolated (30/61, 49.1%), and Staphylococcus hominis the least (1/61, 1.6%). In terms of the number of isolates from the three units examined, the uro-endoscopy unit recorded the highest followed by the general surgical theater and the GI endoscopy. However, there was no association between the various units and bacteria isolated, and no significant difference between the number of isolates among the various units (p = 0.9467, χ2 = 0.1095). In this study, even though CFU per device or device part counted was less than 20, bacteria isolated from the instruments used for a surgical procedure is of great concern considering that the setting of the study is a major teaching hospital. Multi-drug resistance was observed in almost all the isolated bacteria. Sterilization processes should be strictly adhered to, taking into consideration the length and temperature in order to reduce the risk of using contaminated instruments in these environments. It is therefore recommended that similar studies should be carried out in surgical departments at different levels of hospitals to ascertain the extent of this problem.

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“…It is known that antibiotic resistance may be associated with the genetic diversity of some bacterial strains [80][81][82]. In silico analysis of the ribosomal S1 protein revealed its high conservatism.…”

Section: Sequence Analysis Of Ribosomal S1 Proteins Of Model Organism...mentioning

confidence: 99%

Amyloidogenic Peptides: New Class of Antimicrobial Peptides with the Novel Mechanism of Activity

Galzitskaya

Kurpe

Panfilov

et al. 2022

IJMS

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Antibiotic-resistant bacteria are recognized as one of the leading causes of death in the world. We proposed and successfully tested peptides with a new mechanism of antimicrobial action “protein silencing” based on directed co-aggregation. The amyloidogenic antimicrobial peptide (AAMP) interacts with the target protein of model or pathogenic bacteria and forms aggregates, thereby knocking out the protein from its working condition. In this review, we consider antimicrobial effects of the designed peptides on two model organisms, E. coli and T. thermophilus, and two pathogenic organisms, P. aeruginosa and S. aureus. We compare the amino acid composition of proteomes and especially S1 ribosomal proteins. Since this protein is inherent only in bacterial cells, it is a good target for studying the process of co-aggregation. This review presents a bioinformatics analysis of these proteins. We sum up all the peptides predicted as amyloidogenic by several programs and synthesized by us. For the four organisms we studied, we show how amyloidogenicity correlates with antibacterial properties. Let us especially dwell on peptides that have demonstrated themselves as AMPs for two pathogenic organisms that cause dangerous hospital infections, and in which the minimal inhibitory concentration (MIC) turned out to be comparable to the MIC of gentamicin sulfate. All this makes our study encouraging for the further development of AAMP. The hybrid peptides may thus provide a starting point for the antibacterial application of amyloidogenic peptides.

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Occurrence, Antibiotic Resistance, Virulence Factors, and Genetic Diversity of Bacillus spp. from Public Hospital Environments in South Africa

Cited by 9 publications

References 58 publications

Molecular characterization and prevalence of Bacillus species isolated from Saudi hospitals

Molecular characterization and prevalence of Bacillus species isolated from Saudi hospitals

Bacterial Contamination of Surgical Instruments Used at the Surgery Department of a Major Teaching Hospital in a Resource-Limited Country: An Observational Study

Amyloidogenic Peptides: New Class of Antimicrobial Peptides with the Novel Mechanism of Activity

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