Occurrence, location and development of anthocyanoplasts

Pecket, R. C.; Small, Catherine J.

doi:10.1016/s0031-9422(00)83921-7

Cited by 123 publications

(56 citation statements)

References 21 publications

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“…In many plant species, anthocyanins accumulate in the vacuole in discrete structures described by a variety of names (Pecket and Small, 1980;Nozzolillo and Ishikura, 1988;Nozue et al, 1993;Kubo et al, 1995;Markham et al, 2000;Conn et al, 2003;Irani and Grotewold, 2005;Zhang et al, 2006). We found here that intravacuolar anthocyanin-accumulating inclusions are also present in Arabidopsis, particularly in cells induced to accumulate high anthocyanin levels, either as a consequence of the expression of the PAP1 regulator or by the addition of the pathway intermediate, naringenin.…”

Section: Discussionmentioning

confidence: 99%

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Poustka¹,

Irani²,

Feller³

et al. 2007

Plant Physiology

205

230

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Plants produce a very large number of specialized compounds that must be transported from their site of synthesis to the sites of storage or disposal. Anthocyanin accumulation has provided a powerful system to elucidate the molecular and cellular mechanisms associated with the intracellular trafficking of phytochemicals. Benefiting from the unique fluorescent properties of anthocyanins, we show here that in Arabidopsis (Arabidopsis thaliana), one route for anthocyanin transport to the vacuole involves vesicle-like structures shared with components of the secretory pathway. By colocalizing the red fluorescence of the anthocyanins with green fluorescent protein markers of the endomembrane system in Arabidopsis seedlings, we show that anthocyanins are also sequestered to the endoplasmic reticulum and to endoplasmic reticulum-derived vesicle-like structures targeted directly to the protein storage vacuole in a Golgi-independent manner. Moreover, our results indicate that vacuolar accumulation of anthocyanins does not depend solely on glutathione S-transferase activity or ATP-dependent transport mechanisms. Indeed, we observed a dramatic increase of anthocyanin-filled subvacuolar structures, without a significant effect on total anthocyanin levels, when we inhibited glutathione S-transferase activity, or the ATP-dependent transporters with vanadate, a general ATPase inhibitor. Taken together, these results provide evidence for an alternative novel mechanism of vesicular transport and vacuolar sequestration of anthocyanins in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Poustka¹,

Irani²,

Feller³

et al. 2007

Plant Physiology

205

230

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“…The MVT is a transport by vesicles, or structures having membranes, called pre-vacuolar compartments (PVCs), traveling from the RER to the tonoplast (Pecket and Small 1980;Gómez et al 2011). The transport of anthocyanins by PVCs has been described in Vitis vinifera (Conn et al 2003), Arabidopsis thaliana (Poustka et al 2007), and Sorghum bicolor (Snyder and Nicholson 1990).…”

Section: Overview Of Biosynthesis and Transport Of Anthocyaninsmentioning

confidence: 99%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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Main conclusion ABA is involved in anthocyanin synthesis through the regulation of microRNA156, augmenting the level of expression of anthocyanin synthesisrelated genes and, therefore, increasing anthocyanin level.Drought stress is the main cause of agricultural crop loss in the world. However, plants have developed mechanisms that allow them to tolerate drought stress conditions. At cellular level, drought stress induces changes in metabolite accumulation, including increases in anthocyanin levels due to upregulation of the anthocyanin biosynthetic pathway. Recent studies suggest that the higher anthocyanin content observed under drought stress conditions could be a consequence of a rise in the abscisic acid (ABA) concentration. This plant hormone crosses the plasma membrane by specific transporters, and it is recognized at the cytosolic level by receptors known as pyrabactin resistance (PYR)/regulatory component of ABA receptors (PYR/RCARs) that regulate downstream components. In this review, we discuss the hypothesis regarding the involvement of ABA in the regulation of microRNA156 (miRNA156), which is upregulated as part of dehydration stress responsiveness in different species. The miRNA156 upregulation produces a greater level of anthocyanin gene expression, forming the multienzyme complex that will synthesize an increased level of anthocyanins at the cytosolic face of the rough endoplasmic reticulum (RER). After synthesis, anthocyanins are transported from the RER to the vacuole by two possible models of transport: (1) membrane vesicle-mediated transport, or (2) membrane transporter-mediated transport. Thus, the aim was to analyze the recent findings on synthesis, transport and the possible mechanism by which ABA could increase anthocyanin synthesis under drought stress conditions potentially throughout microRNA156 (miRNA156).

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“…In certain plant species, anthocyanins are however localized in discrete regions of the cell vacuole, called anthocyanoplasts (Pecket and Small 1980). The content of anthocyanin ( (delphinidin, cyanidin and pelargonidin) are the most abundant in nature, representing 80, 69 and 50 % of leaf, fruit and flower pigments, respectively.…”

Section: Anthocyanin Concentration and Distribution In The Fruitmentioning

confidence: 99%

Fruit skin color and the role of anthocyanin

et al. 2013

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Fruit skin coloration is a unique phase in the life cycle of fruiting plants and is mainly attributed to anthocyanin pigments. Anthocyanins are the largest and most diverse group of plant pigments derived from the phenyl propanoid pathway. They are water-soluble phenolic compounds that form part of a large and common group of plant flavonoids. Coloration encompasses several physiological and biochemical changes that happen through differential expression of various developmentally regulated genes. Due to research importance and economic value, Arabidopsis thaliana (chromosome no. = 5) and Vitis vinifera (chromosome no. = 19) have been used for investigations of the structural genes involved in anthocyanin biosynthesis. Thus for this review, V. vinifera is used as a model crop. In anthocyanin biosynthesis, a wide range of constructive genes including phenylalanine ammonia lyase, chalcone synthase and anthocyanidin synthase that are regulated by MYB transcription factors are involved. These genes are coordinately expressed and their levels of expression are positively related to the anthocyanin concentrations. Expression or suppression of the constructive genes contributes to a variety of changes that make fruits visually attractive and edible. Transgenic approaches also have discovered a strong relationship between phenyl propanoid/flavonoid gene expressions for fruit skin coloration. In this study, various developments that have taken place in the last decade with respect to identifying and altering the function of color-related genes are described.

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Occurrence, location and development of anthocyanoplasts

Cited by 123 publications

References 21 publications

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

Fruit skin color and the role of anthocyanin

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