Occurrence of aflatoxin- and ochratoxin A-producing Aspergillus species in Thai dried chilli

Chuaysrinule, Chananya; Maneeboon, Thanapoom; Roopkham, Chanram; Mahakarnchanakul, Warapa

doi:10.1016/j.jafr.2020.100054

Cited by 25 publications

(24 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All the isolates of A. flavus and A. carbonarius have been proven to produce AFB 1 and OTA, respectively, and A. oryzae was proven to produce neither of them. The analysis was conducted using thin-layer chromatography (TLC) coupled with densitometry following extraction with the protocol described by Chuaysrinule, Maneeboon, Roopkham and Mahakarnchanakul [15].…”

Section: Fungal Strainsmentioning

confidence: 99%

See 1 more Smart Citation

Interactive Inhibition of Aflatoxigenic Aspergillus flavus and Ochratoxigenic Aspergillus carbonarius by Aspergillus oryzae under Fluctuating Temperatures

Dwipa

Maneeboon

Chuaysrinule

et al. 2022

Microbiology Research

Self Cite

View full text Add to dashboard Cite

This study aimed to evaluate the effectiveness of A. oryzae in inhibiting aflatoxin B1 (AFB1) and ochratoxin A (OTA) production by A. flavus and A. carbonarius, respectively, under shifting temperatures. A. oryzae was tested on different agar, namely coconut cream agar (CCA) and chili-based agar to figure out the variation in the effectiveness of A. oryzae on the most appropriate medium for A. flavus and A. carbonarius to produce mycotoxin and under natural condition where they are predominantly found. On CCA, the temperatures applied were 20, 30, 35, 40, 20/30, 20/35, and 20/40 °C, while on chili-based agar, the temperatures imposed were 20, 40, and 20/40 °C, at varied water activity of 0.92 and 0.97aw. The findings indicated that A. oryzae was much more effective in inhibiting the growth of A. flavus rather than A. carbonarius, yet it was able to inhibit higher OTA concentration than AFB1 at fluctuating temperatures on CCA as the most appropriate medium for A. flavus and A. carbonarius. A. oryzae effectively inhibited AFB1 and OTA at static temperature of 20 °C and water activity of 0.97aw on chili-based agar. Under fluctuating temperatures (20/40 °C), A. oryzae was also able to control mycotoxin, particularly OTA at high water activity (0.97aw).

show abstract

Section: Fungal Strainsmentioning

confidence: 99%

“…Previous studies have demonstrated the occurrence of aflatoxins and OTA in chili from several countries, such as Indonesia, Malaysia, Pakistan, Bangladesh, Qatar, Saudi Arabia, and Thailand. In addition, mostly isolated fungi from chili include A. parasiticus, A. flavus, A. nomius, and A. niger [9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Interactive Inhibition of Aflatoxigenic Aspergillus flavus and Ochratoxigenic Aspergillus carbonarius by Aspergillus oryzae under Fluctuating Temperatures

Dwipa

Maneeboon

Chuaysrinule

et al. 2022

Microbiology Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…3). These species, among others belonging to sections Nigri and Flavi, have been shown to be predominant fungal contaminants in Capsicum pods and derivative products, being associated with the presence of mycotoxins in such food items (Chuaysrinule et al, 2020;Costa et al, 2019b;Frimpong et al, 2019;Ham et al, 2016).…”

Section: Mycobiota Isolation and Identificationmentioning

confidence: 99%

Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén

Costa

Rodriguez

Santos

et al. 2020

International Journal of Food Microbiology

View full text Add to dashboard Cite

This work aims to provide the first study on the mycobiota present in Chilean pepper Capsicum annuum L. cv. "Cacho de Cabra" throughout the early production stages. Two hundred and forty berry fruits were sampled: 1) at the ripe fruits harvest day; 2) during drying; and 3) smoking processes. A total of 192 strains, encompassing 11 genera and 44 species, were identified through analysis of β-tubulin (benA) gene and internal transcribed spacer of ribosomal DNA (ITS) region. All collection points showed samples with high fungal contamination, but the mycobiota composition varied as a result of different environmental conditions. Alternaria spp. and Fusarium spp. were predominantly isolated from fresh fruits of C. annuum. Penicillium spp. was the most frequent genus in all analysed points. Penicillium brevicompactum and P. crustosum were the most abundant species. Among Aspergillus, A. niger and A. flavus were dominant after the drying phase. In our study, none of the analysed strains of Penicillium (113) and Aspergillus (35) produced Ochratoxin A at detectable levels. The broad characterization of the fungal community of C. annuum carried out in this study, could be a guideline for future mycotoxin analyses performed directly on the pod. Understanding the role and dynamics of mycobiota and its relationship with the toxins present in this substrate, will be useful to establish and improve control measures considering the specificities of each point in the C. annuum production chain.

show abstract

“…Apart from the main OTA toxigenic species A. ochraceus and A. westerdijkiae, several other Aspergillus species have been linked to OTA production, such as A. sclerotiorum, A. sulphureus, A. albertensis, A. auricomus, A. wentii, A. fumigates, A. versicolor, A. cretensis, A. flocculosus, A. pseudoelegans, A. roseoglobulosus, A. sulphurous [39], A. alliaceus [40], A. welwitschiae (formerly A. awamori) [41], A. affinis [42], A. steynii [43], A. lacticoffeatus, A. sclerotioniger [44] and A. carbonarius [45].…”

Section: Production Of Ota 21 General Factorsmentioning

confidence: 99%

Ochratoxin A in Slaughtered Pigs and Pork Products

Vlachou

Pexara

Σολωμάκος

et al. 2022

Toxins

View full text Add to dashboard Cite

Ochratoxin A (OTA) is a mycotoxin that is produced after the growth of several Aspergillus and Penicillium spp. in feeds or foods. OTA has been proved to possess nephrotoxic, hepatotoxic, teratogenic, neurotoxic, genotoxic, carcinogenic and immunotoxic effects in animals and humans. OTA has been classified as possibly carcinogenic to humans (Group 2B) by the IARC in 2016. OTA can be mainly found in animals as a result of indirect transmission from naturally contaminated feed. OTA found in feed can also contaminate pigs and produced pork products. Additionally, the presence of OTA in pork meat products could be derived from the direct growth of OTA-producing fungi or the addition of contaminated materials such as contaminated spices. Studies accomplished in various countries have revealed that pork meat and pork meat products are important sources of chronic dietary exposure to OTA in humans. Various levels of OTA have been found in pork meat from slaughtered pigs in many countries, while OTA levels were particularly high in the blood serum and kidneys of pigs. Pork products made from pig blood or organs such as the kidney or liver have been often found to becontaminated with OTA. The European Union (EU) has established maximum levels (ML) for OTA in a variety of foods since 2006, but not for meat or pork products. However, the establishement of an ML for OTA in pork meat and meat by-products is necessary to protect human health.

show abstract

Occurrence of aflatoxin- and ochratoxin A-producing Aspergillus species in Thai dried chilli

Cited by 25 publications

References 56 publications

Interactive Inhibition of Aflatoxigenic Aspergillus flavus and Ochratoxigenic Aspergillus carbonarius by Aspergillus oryzae under Fluctuating Temperatures

Interactive Inhibition of Aflatoxigenic Aspergillus flavus and Ochratoxigenic Aspergillus carbonarius by Aspergillus oryzae under Fluctuating Temperatures

Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén

Ochratoxin A in Slaughtered Pigs and Pork Products

Contact Info

Product

Resources

About