Occurrence of aflatoxin M₁in milk and dairy products commercialized in Campinas, Brazil

Abstract

One hundred and fifty-two samples of pasteurized milk, powdered milk, cheese and yoghurt, marketed in Campinas, Brazil in 1989-1990, were analysed for aflatoxin M1 by the AOAC TLC method (visual quantitation) 980.21. Fifty-two pasteurized milk samples were also analysed in 1992 by the AOAC HPLC method 986.16. Aflatoxin M1 was not detected in the 1989-1990 samples. Four milk samples of the 1992 batch were contaminated at 73-370 ng/1. Except for the sample with 370 ng/l, which would have been also found positive by the TLC method, the detection of aflatoxin M1 in 1992 reflects the higher sensitivity of the HPLC method, not a greater occurrence of the toxin. Contamination of milk and milk products with aflatoxin M1 does not appear to be a serious public health problem in the city of Campinas at the moment.

Occurrence of aflatoxin M1 in pasteurised, UHT milk and milk powder from goat origin

“…In the state of São Paulo, Martins and Martins (1986) observed four samples (1.8%) of pasteurised milk containing AFM 1 , with two of them containing 0.250 lg L À1 . Sabino, Purchio, and Zorzetto (1989) detected AFM 1 (0.200 lg L À1 ) in only one sample (1.0%) of pasteurised milk, whereas Sylos, Rodriguez-Amaya, and Carvalho (1996) observed four samples (7.7%) with AFM 1 levels above 0.050 lg L À1 (maximum of 0.370 lg L À1 ). Recently, Garrido, Iha, Santos, and Duarte (2003) observed 29 samples (20.9%) of pasteurised and UHT milk with AFM 1 at levels ranging from 0.050 to 0.240 lg L À1 .…”

Determinação de aflatoxina B1 em rações e aflatoxina M1 no leite de propriedades do Estado de São Paulo

“…Em seguida, passou-se 1 mL de metanol, de maneira a eluir a AFM 1 , recolhendo-se o eluato em frasco âmbar. O eluato foi diluído com água ultrapurificada até formar uma solução de metanol-PURCHIO; ZORZETTO, 1989;SYLOS;RODRIGUEZ-AMAYA;CARVALHO, 1996), mas os níveis observados geralmente se encontram abaixo do limite de tolerância (0,5 µg.L -1 ) determinado pelas normas vigentes (BRASIL, 2002a). A ocorrência de AFB 1 em ingredientes para rações do gado leiteiro é relativamente frequente no Brasil (RODRÍGUEZ-AMAYA; SABINO, 2002), porém são poucos os trabalhos que avaliam a ocorrência de AFB 1 na ração fornecida ao gado leiteiro e de AFM 1 no leite em condições de campo.…”

Occurrence of aflatoxin M1 in parmesan cheese consumed in Minas Gerais, Brazil

“…Few surveys have been undertaken to assess the level of AFM 1 in some types of cheese consumed in Brazil (Table 2). Sylos et al (1996) detected no AFM 1 in cheese commercialized, in 1990, in Campinas/Brazil. However, the analytical method employed was a thin-layer chromatography which is less sensitive than high-performance liquid chromatography (HPLC).…”

“…In Brazil there are limited surveys for AFM 1 in milk (GARRIDO et al, 2003;OLIVEIRA et al, 1997OLIVEIRA et al, , 2006PARREIRAS & GOMES, 1987;PEREIRA et al, 2005;PRADO et al, 1999;SABINO et al, 1989;SOUZA et al, 1999;SYLOS et al, 1996;TAVEIRA & MÍDIO, 2001) and milk products as yoghurt and cheese (PRADO et al, 2000(PRADO et al, , 2001SYLOS et al, 1996). AFM 1 is relatively stable during pasteurization, sterilization, preparation, and storage of various dairy products (GALVANO et al, 1996;GÜRBAY et al, 2006;HASSANIN, 1994).…”