Occurrence of albinism during wheat androgenesis is correlated with repression of the key genes required for proper chloroplast biogenesis

Canonge, Julie; Roby, Charlotte; Hamon, Céline; Potin, Philippe; Pfannschmidt, Thomas; Philippot, Murielle

doi:10.1007/s00425-021-03773-3

Cited by 5 publications

(5 citation statements)

References 78 publications

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“…Genotypic dependency of albinism is associated with transcription activation of specific genes involved in chloroplast biogenesis at early stages (Mozgova et al, 2006;Canonge et al, 2021). Chloroplast DNA deletions were observed in albino plants, along with inhibited transcription of the nuclear genes coding for chloroplast-localized proteins, while levels of transcripts coding for proteins not present in chloroplasts were identical to those in green plants (Dunford, Walden, 1991).…”

Section: Resultsmentioning

confidence: 99%

Study of wheat (Triticum aestivum L.) breeding material potential for (Triticum aestivum L.)androgenesis

Petrash,

Kapko,

Sovetov

2023

Vestn. VOGiS

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Doubled haploid technology is a valuable biotechnological approach in plant breeding that enables one to quickly create new varieties through the single-stage production of homozygous lines. The aim of this study was to assess the indicators of in vitro androgenesis in the anther culture of the initial breeding material of varieties and combinations of F1 and F2 and to identify promising accessions with good responsiveness. For that purpose, the plant material that proved promising for the breeding programs of Siberian Research Institute of Plant Production and Breeding (SibRIPP&B) was used. Ten cultivars of common wheat and the F1 and F2 hybrids of nine combinations were evaluated for the main parameters of in vitro androgenesis such as the number of new formations, albino, green and all regenerated plants. Induction of androgenesis in vitro was carried out in anther culture in growth medium Chu (N6) containing 1 mg/l of growth regulator 2,4-D. The studied samples showed different responses to induction. The maximum level of new formations was found in F2 hybrids Novosibirskaya 15 × Lutescens ShT-335. The largest number of green plants was found in F1 Novosibirskaya 15 × Lutescens ShT-335. According to the results of variance analysis, a significant ( p < 0.01) influence of genotype on the studied traits was established. Varieties with good responsiveness to anther culture (Novosibirskaya 15) and lack of responsiveness to in vitro androgenesis (Novosibirskaya 31) were identified. Novosibirskaya 16 was characterized by a low regeneration capacity of new formations. A significant heterotic effect was revealed considering the number of new formations per 100 anthers among the hybrids of such combinations as Novosibirskaya 15 × Lutescens ShT-335, Novosibirskaya 15 × Lutescens 111/09, and Zagora Novosibirskaya × Obskaya 2. Novosibirskaya 15 was recommended for inclusion in crossings as a parental form that provides high hybrid responsiveness during in vitro androgenesis. The use of doubled haploid technology made it possible to quickly create DH-lines based on the breeding material.

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Section: Resultsmentioning

confidence: 99%

Study of wheat (Triticum aestivum L.) breeding material potential for (Triticum aestivum L.)androgenesis

Petrash,

Kapko,

Sovetov

2023

Vestn. VOGiS

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“…Future research may aim for instance to understand whether the formation of chloroplasts in resurrection plants after re-watering follows the same principles as the chloroplast-eoplast-chloroplast transition in embryos of Arabidopsis seeds and whether this is relevant for drought tolerance (Ingle et al 2008;Allorent et al 2013;Solymosi et al 2013). Understanding the impact of retrograde signals may also help to solve problems in current agricultural double-haploid breeding technologies that often suffer from high proportions of albino plantlets that emerge during androgenesis because of disturbed chloroplast biogenesis (Gajecka et al 2020(Gajecka et al , 2021Canonge et al 2021). Finally, chloroplasts have been identified as a reliable and safe future production platform for recombinant proteins, pharmaceutics and many other bioproducts, but their biotechnological potential is currently far from being efficiently used (Scharff and Bock 2014;Newkirk et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Biogenic signals from plastids and their role in chloroplast development

Liebers

Cozzi

Uecker

et al. 2022

Journal of Experimental Botany

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Plant seeds do not contain differentiated chloroplasts. Upon germination the seedling, thus, need to gain photoautotrophy before storage energies are depleted. This requires the coordinated expression of photosynthesis genes encoded in nuclear and plastid genomes. Chloroplast biogenesis needs to be additionally coordinated with the light regulation network that controls seedling development. This coordination is achieved by nucleus-to-plastid signals called anterograde and plastid-to-nucleus signals coined retrograde. Retrograde signals sent from plastids during intial chloroplast biogenesis are also called biogenic signals. They have been recognized as highly important for proper chloroplast biogenesis and for seedling development. The molecular nature, transport, targets and signalling function of biogenic signals are, however, under debate. Several studies disproved the involvement of a number of key components that were at the base of initial models of retrograde signalling. New models now propose major roles for a functional feedback between plastid and cytosolic protein homeostasis in signaling plastid dysfunction as well as the action of dually localized nucleo-plastidic proteins that coordinate chloroplast biogenesis with light-dependent control of seedling development. This review provides a survey of the developments in this research field, summarizes the unsolved questions, high-lights several recent advances and discusses potential new working modes.

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“…Horticulturae 2022, 8, x FOR PEER REVIEW 16 of 30 and wheat (Triticum aestivum L.) suggests that the albino chlorosis is due to genetic mutations that impacts plastid production, function and/or carotenoid synthesis, or it may be due to photooxidative damage occur to chloroplasts [129]. Recently, it was found that albinism in wheat is linked to the transcriptional suppression of the specific genes responsible for chloroplast biosynthesis during the first weeks of in vitro anther culture [130]. Adding copper sulfate at 10 μM to anther pre-medium improved the regeneration of green doubled haploid plants from cultivars known to produce mainly albino plants, when the ratio of albino plants is reduced.…”

Section: Albino Plantlets (Albinism) In Anther Culturesmentioning

confidence: 99%

“…Current research on oat (Avena sativa L.), barley (Hordeum vulgare L.), and wheat (Triticum aestivum L.) suggests that the albino chlorosis is due to genetic mutations that impacts plastid production, function and/or carotenoid synthesis, or it may be due to photooxidative damage occur to chloroplasts [129]. Recently, it was found that albinism in wheat is linked to the transcriptional suppression of the specific genes responsible for chloroplast biosynthesis during the first weeks of in vitro anther culture [130].…”

Section: Recalcitrance In Clonal Micropropagationmentioning

confidence: 99%

An Academic and Technical Overview on Plant Micropropagation Challenges

et al. 2022

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The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

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Occurrence of albinism during wheat androgenesis is correlated with repression of the key genes required for proper chloroplast biogenesis

Cited by 5 publications

References 78 publications

Study of wheat (<i>Triticum aestivum</i> L.) breeding material potential for (<i>Triticum aestivum</i> L.)androgenesis

Study of wheat (<i>Triticum aestivum</i> L.) breeding material potential for (<i>Triticum aestivum</i> L.)androgenesis

Biogenic signals from plastids and their role in chloroplast development

An Academic and Technical Overview on Plant Micropropagation Challenges

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