Occurrence of antibiotics in wastewater from hospital and convectional wastewater treatment plants and their impact on the effluent receiving rivers: current knowledge between 2010 and 2019

Oharisi, Omuferen-oke Loveth; Maseko, Bethusile Rejoice; Olowoyo, Joshua Oluwole

doi:10.1007/s10661-022-09846-4

Cited by 73 publications

(30 citation statements)

References 70 publications

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“… 8–10 Hospital sewage is generally regarded as a reservoir of antibiotic-resistant bacteria, and the concentration of azithromycin in hospital sewage could be high. 11 However, little information about the prevalence of azithromycin-resistant Vibrio species from hospital sewage is available. In this study, we report one V. furnissii isolate recovered from hospital sewage in Zhuhai, a coastal city in China, carrying both mph (A) and bla OXA-1 , which are azithromycin and cephalosporin resistance genes, respectively.…”

Section: Short Reportmentioning

confidence: 99%

Emergence of blaOXA-1- and mph(A)-Producing Vibrio furnissii Isolated from Hospital Sewage

Mo¹,

Lin²,

Liu³

et al. 2022

IDR

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Cephalosporins and azithromycin are clinical antibiotics used to treat infections. Co-resistance to cephalosporins and azithromycin has been observed in some Enterobacterales , but it has only rarely been reported in Vibrio species. In this study, we isolated a cephalosporin- and azithromycin-resistant V. furnissii strain, VFN3, from hospital sewage. Whole-genome sequencing results showed that the strain VFN3 possesses an IncA/C2 plasmid, pVFN3-blaOXA-193K. This conjugative plasmid carries several clinically relevant drug resistance genes, including mph (A) and bla OXA-1 . We also found that in the strain VFN3, mph (A) and bla OXA-1 are surrounded by insertion sequences and class I integrons, respectively. These data suggest that mobile elements mediate the transfer of mph (A) and bla OXA-1 . This is the first reported Vibrio species that possesses an mph (A)- and bla OXA-1 -bearing conjugative plasmid. The emergence of this conjugative multi-drug-resistance plasmid is of great concern to public health.

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Section: Short Reportmentioning

confidence: 99%

Emergence of blaOXA-1- and mph(A)-Producing Vibrio furnissii Isolated from Hospital Sewage

Mo¹,

Lin²,

Liu³

et al. 2022

IDR

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“…Intestinal microbes are essential for digestion, metabolism, and immune activities in all animals [ 18 , 19 , 20 , 21 , 22 ]. In addition, aquatic environments are continuously exposed to antibiotics owing to persistent residues in the environment [ 23 , 24 ]. The resulting development of drug-resistant microbes, which are the primary ARG hosts, and ABR bacteria development in aquatic environments, increase the risks of gastrointestinal illnesses caused by such bacteria [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Resource Availability and Antibiotic Residues on Intestinal Antibiotic Resistance in Bellamya aeruginosa

Xiao

Zhang

et al. 2023

Microorganisms

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Widespread and inappropriate use of antibiotics has been shown to increase the spread of antibiotics and antimicrobial resistance genes (ARGs) in aquatic environments and organisms. Antibiotic use for the treatment of human and animal diseases is increasing continuously globally. However, the effects of legal antibiotic concentrations on benthic consumers in freshwater environments remain unclear. In the present study, we tested the growth response of Bellamya aeruginosa to florfenicol (FF) for 84 days under high and low concentrations of sediment organic matter (carbon [C] and nitrogen [N]). We characterized FF and sediment organic matter impact on the bacterial community, ARGs, and metabolic pathways in the intestine using metagenomic sequencing and analysis. The high concentrations of organic matter in the sediment impacted the growth, intestinal bacterial community, intestinal ARGs, and microbiome metabolic pathways of B. aeruginosa. B. aeruginosa growth increased significantly following exposure to high organic matter content sediment. Proteobacteria, at the phylum level, and Aeromonas at the genus level, were enriched in the intestines. In particular, fragments of four opportunistic pathogens enriched in the intestine of high organic matter content sediment groups, Aeromonas hydrophila, Aeromonas caviae, Aeromonas veronii, and Aeromonas salmonicida, carried 14 ARGs. The metabolic pathways of the B. aeruginosa intestine microbiome were activated and showed a significant positive correlation with sediment organic matter concentrations. In addition, genetic information processing and metabolic functions may be inhibited by the combined exposure to sediment C, N, and FF. The findings of the present study suggest that antibiotic resistance dissemination from benthic animals to the upper trophic levels in freshwater lakes should be studied further.

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“…For that purpose, an in silico design of the different primer sets was made based on the alignment of sequences retrieved from the Kyoto Encyclopedia of Genes and Genome (KEGG) database with an orthology grade to the reference sequence > 70%. In addition, the molecular tools and the new qPCR protocols here developed were validated in vivo using DNA extracted from different environmental samples, including activated sludge, river sediment, and agricultural soils, ecosystems previously described as hotspots of the dissemination of the targeted ARGs and as environmental reservoirs of ARB [3,[27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Design and validation of primer sets for the detection and quantification of antibiotic resistance genes in environmental samples by quantitative PCR

Perez-Bou

González‐Martínez

Cabrera

et al. 2023

Preprint

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The high prevalence of antibiotic resistant bacteria (ARB) in several environments is a great concern threatening human health. Hence, it is vital to dispose of molecular tools that allow proper monitoring of antibiotic resistant genes (ARGs) encoding resistances to these important therapeutic compounds. For an accurate quantification of ARGs, there is a need for sensitive and robust qPCR assays supported by a good design of primers and validated protocols. In this study, eleven relevant ARGs were selected as targets, including aadA and aadB (conferring resistance to aminoglycosides), ampC, blaTEM, blaSHV, and mecA (resistance to beta-lactams); dfrA1 (resistance to trimethoprim); ermB (resistance to macrolides); fosA (resistance to fosfomycin); qnrS (resistance to quinolones); and tetA(A) (resistance to tetracyclines). The in silico design of the new primer sets was performed based on the alignment of all the sequences of the target ARGs (orthology grade > 70%) deposited in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, allowing higher coverages of the ARG’s biodiversity than those of several primers described to date. The adequate design and well performance of the new molecular tools were validated in vivo in six samples, retrieved from both natural and engineered environments. The hallmarks of the optimized qPCR assays were high amplification efficiency (> 90%), good linearity of the standard curve (R2 > 0.980), consistency across replicate experiments, and a wide dynamic range. The new methodology described here provide valuable tools to upgrade the monitorization of the abundance and emergence of the targeted ARGs in the environment by qPCR.

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Occurrence of antibiotics in wastewater from hospital and convectional wastewater treatment plants and their impact on the effluent receiving rivers: current knowledge between 2010 and 2019

Cited by 73 publications

References 70 publications

Emergence of blaOXA-1- and mph(A)-Producing Vibrio furnissii Isolated from Hospital Sewage

Emergence of blaOXA-1- and mph(A)-Producing Vibrio furnissii Isolated from Hospital Sewage

Effects of Resource Availability and Antibiotic Residues on Intestinal Antibiotic Resistance in Bellamya aeruginosa

Design and validation of primer sets for the detection and quantification of antibiotic resistance genes in environmental samples by quantitative PCR

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