Occurrence of Antibodies to Paracoccidioides brasiliensis in Dairy Cattle from Mato Grosso do Sul, Brazil

Abstract

The aim of this study was to evaluate the humoral immune response in cattle immunized with Paracoccidioides brasiliensis and perform a seroepidemiological study of paracoccidioidomycosis in dairy cattle from Mato Grosso do Sul. Two animals (one steer and one heifer) were inoculated with a suspension of P. brasiliensis in Freund incomplete adjuvant. Blood samples were collected periodically to evaluate humoral immune response by immunodiffusion and ELISA, using exoantigen and gp43 as antigens, respectively. The antibody production was detected by immunodiffusion and ELISA, in both animals, 14 days after immunization. The soroepidemiologic study was carried out in 400 cattle of Mato Grosso do Sul from four municipalities: Corumbá, Dourados, Nova Andradina, and São Gabriel d'Oeste. The municipalities of Corumbá (30%) and Nova Andradina (28%) showed higher positivity than Dourados (8%) and São Gabriel d'Oeste (4%). In this study we concluded that cattle immunized with P. brasiliensis develop humoral immune response for gp43, remaining with high titers of antibodies, and that this animal species could be an epidemiologic marker of paracoccidioidomycosis.

Paracoccidioides brasiliensis Infection in Small Wild Mammals

“…Epidemiological studies of PCM in domestic and wild animals have been based mainly on immunological reactions using crude paracoccidioidin and purified gp43 as antigens [5][6][7][8][9][10][11][12][13][14][15][16]32]. Previously, our research group showed high rates of gp43 ELISA positivity in dogs [6], horses [9], sheep [12], goats [13], pigs [29], rabbits [30] and wild monkeys [15] from Paraná State, Brazil.…”

“…presence in endemic areas. Infection by P. brasiliensis has been reported in domestic and wild animals such as dogs [5][6][7], horses in Uruguay and in Brazil [8,9], cows in Colombia and Brazil [10,11], sheep, goats and chickens in Brazil [12][13][14], and monkeys [15] and armadillos in Brazil [16]. P. brasiliensis isolates were obtained from soil samples in Argentina [17], Venezuela [18] and Brazil [19].…”

Detecção de anticorpos para Paracoccidioides brasiliensis em cavalos da região norte do Estado do Paraná

“…Recentemente esta técnica passou a ser utilizada também em estudos epidemiológicos de paracoccidioidomicose em humanos (BOTTEON et al, 2002), cães (ONO et al, 2001;SILVEIRA et al, 2006), macacos (CORTE et al, 2007) e bovinos (SILVEIRA et al, 2008).…”

“…A infecção por P. brasiliensis tem sido demonstrada em diferentes espécies de animais domésticos como cães, bovinos, eqüinos e silvestres como macacos e felinos (CORTE et al, 2007;MÓS;FAVA NETTO, 1974;ONO et al, 2001;SILVEIRA et al, 2006SILVEIRA et al, , 2008. Considerando a escassez de dados sobre a ecoepidemiologia da paracoccidioidomicose, este estudo teve como objetivo avaliar a infecção por P. brasiliensis em cavalos da Região Norte do Estado do Paraná.…”

Paracoccidioides brasiliensis infection in dogs from Western Brazilian Amazon

“…Epidemiological studies have shown that paracoccidioidomycosis occurs in several species of domestic and wild animals such as dogs (Mós & Fava-Netto 1974, Ono et al 2001, Silveira et al 2006, Canteros et al 2010, Fontana et al 2010, cows (Silveira et al 2008), horses (Conti Diaz et al 1972, Costa & Fava-Netto 1978, Corte et al 2009), armadillos (Fernandes et al 2004), sheep (Costa & Fava-Netto 1978, Oliveira et al 2011, monkeys (Corte et al 2007), guinea pigs, raccoons, grisons, porcupine (Richini-Pereira et al 2008) and chickens (Gonçalves et al 2011). The fungus was also isolated from armadillos (Naiff et al 1986, Bagagli et al 1998, Corredor et al 1999, Silva-Vergara et al 2000 penguin faeces (Gezuele 1989), and recently, natural paracoccidioidomycosis was reported in two dogs (Ricci et al 2004, Farias et al 2011), a cat (Gonzalez et al 2010) and a two-toed sloth (Trejo-Chávez et al 2010).…”