Occurrence of antimicrobial-resistant Enterobacteriaceae in water from different sources in a subtropical region of Argentina

Lösch, Liliana Silvina; Alonso, Justo; Merino, Luis Antonio

doi:10.4136/ambi-agua.50

Cited by 13 publications

(8 citation statements)

References 23 publications

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“…Shrestha (2008) also found high resistance among drinking water isolates against Ampicillin (93.9%). Losch et al (2008) in Argentina and Tambedkar et al (2008) in India found that Ampicillin was least effective antibiotic for water isolates. Our study indicated higher percentage of AR and MAR bacteria in drinking water samples.…”

Section: The Mean Free Residual Chlorine (Frc) Mean Combinedmentioning

confidence: 97%

Evaluation of Physicochemical and Microbiological Parameters of Drinking Water Supplied from Distribution Systems of Kathmandu Municipality

Shakya

Joshi

et al. 2013

Nepal Journal of Science and Technology

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Microbial pathogens in drinking water are primary cause in gastrointestinal and waterborne diarrheal diseases. Public health authorities still have not achieved success in controlling the most common waterborne diseases in Nepal. The objective of this study was to assess the quality of drinking water supplied from distribution system of Kathmandu. A total of 114 water samples were collected from 4 distribution stations. The physicochemical and microbiological analyses of water were conducted. Heterotrophic plate count (HPC), detection of enteric pathogenic bacteria and antibiotics susceptibility test were done. Distinct variations in physicochemical parameters were observed. The mean free residual chlorine was found 0.24mg/l. Total Coliforms were found in 61.4% (70/114) of water samples among which 15.7% (11/70) had thermotolerant Coliforms. In distance, coliforms were found higher in far points (p=0.002). All thermotolerant Coliforms were identified as Escherichia coli. Mean HPC of water was found log103.15. The mean HPC of water (p=0.003) was found higher in far point. In collected samples, 10 different enteric bacteria were isolated in which E. coli was found most predominant followed by Pseudomonas spp., Citrobacter spp., Klebsiella spp., Shigella spp., Proteus spp., Enterobacter spp. Non-Typhii Salmonella spp., Providencia spp., Edwardsiella spp. In antibiotics susceptibility test, Gentamicin and Ofloxacin were found most efficient (100%) and whereas Ampicillin (11.5%) was least effective. Multiple antibiotics resistance was found among 5.3% of isolates. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 179-184 DOI: http://dx.doi.org/10.3126/njst.v13i2.7733

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Section: The Mean Free Residual Chlorine (Frc) Mean Combinedmentioning

confidence: 97%

Evaluation of Physicochemical and Microbiological Parameters of Drinking Water Supplied from Distribution Systems of Kathmandu Municipality

Shakya

Joshi

et al. 2013

Nepal Journal of Science and Technology

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“…The isolates also displayed resistance to multiple antibiotics (4 to 7). Bacteria from these genera have been reported to possess multidrug resistance 38,39 . Six (6) multi-drug resistant bacteria isolated from household water samples were analyzed for plasmid out of which 4 harbored more than one plasmid.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Pattern and Plasmid Profile of Bacteria Isolates from Household Water Distribution Tanks in Ado-Ekiti

Babalola¹,

Olowomofe²,

Omodara³

et al. 2021

J. Pure Appl. Microbiol.

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Water is essential to life. The existence of all forms of life is dependent on an adequate water supply. The exigent need for water supply in homes prompted the construction of water sources and water storage devices in the homes. This however does not guarantee that the water is safe to drink. If the water is safe at the source, it may be contaminated during transportation storage and drawing at home. This study was carried out to determine the microbial counts, antibiotics susceptibility and plasmid profile of bacteria isolates from household water distribution tanks in the Ado-Ekiti metropolis. The total bacteria and coliform counts were determined using the pour plating technique. The antibiotic susceptibility pattern of the isolates was determined using the disc diffusion technique while the plasmid profile of the isolates was determined using the alkaline lysis method and agar gel electrophoresis. The mean total bacteria count of the water sample was 6.96 log10 CFU/ml, while the mean total of coliform count is 5.50 log10CFU/ml. The isolates with multiple antibiotics resistance belonged to five bacteria genera namely: Escherichia, Pseudomonas, Klebsiella, Enterobacter and Proteus. The plasmid analysis showed that four of the resistant strains had multiple plasmids, Enterobacter aerogens had 3 plasmids (1kb, 1.5kb and 2kb), Pseudomonas aeruginosa and Klebsiella aerogens had two plasmids (1kb, 1.5kb) respectively while Proteus vulgaris and Escherichia coli had no plasmid.

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“…Samples were collected in sterile 250-ml polypropylene bottles, according to internationally recommended methodology [10]. Samples were kept at 4°C until their arrival to laboratory.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistant coliform bacteria in the Gomti river water and determination of their tolerance level

2014

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The distribution of resistance to ampicillin, chloramphenicol, sulfonamides, tetracycline, and streptomycin among coliform in the Gomti river water samples was investigated. The coliform populations were isolated on Mac Conky and eosin methylene blue (EMB) agar plates supplemented with antibiotics. The incidence of resistance among the coliform population varied considerably in different drug and water sampling sites. Coliform bacteria showed lower drug resistant viable count in sampling site-III (receiving treated wastewater) as compared to more polluted site-I and site-II. Viable count of coliform population obtained on both medium was recorded higher against erythromycin from sampling site-III. Lower viable count of coliforms was recorded against tetracycline in site-II and III. Similar resistance pattern was obtained in the frequency of E. coli and Enterobacter species from all the three sampling sites. Percentage of antibiotic resistant E. coli was observed higher than Enterobacter spp among the total coliforms against all antibiotics tested without Erythromycin and penicillin in site-I and II respectively. Isolates of E. coli and Enterobacter spp. showed their tolerance level (MIC) in the range of 2-100 against the antibiotics tested. Maximum number of isolates of both genus exhibited their MICs at lower concentration range 2-5µg/ml against ciprofloxacin, tetracyclin and amoxycillin.AbbreviationsEMB - Eosin methylene blue, IMViC tests - Indole, Methyl Red, Voges Proskauer and Citrate Utilization Tests, MIC - Minimum inhibitory concentration.

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Occurrence of antimicrobial-resistant Enterobacteriaceae in water from different sources in a subtropical region of Argentina

Cited by 13 publications

References 23 publications

Evaluation of Physicochemical and Microbiological Parameters of Drinking Water Supplied from Distribution Systems of Kathmandu Municipality

Evaluation of Physicochemical and Microbiological Parameters of Drinking Water Supplied from Distribution Systems of Kathmandu Municipality

Antibiotic Resistance Pattern and Plasmid Profile of Bacteria Isolates from Household Water Distribution Tanks in Ado-Ekiti

Antimicrobial resistant coliform bacteria in the Gomti river water and determination of their tolerance level

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