Occurrence of Aviadenovirus in chickens from the poultry industry of Minas Gerais

Abstract

The occurrence of Aviadenovirus (FAdV) was investigated in chickens from the poultry industry of Minas Gerais state, Brazil. The investigation was conducted due to the scarcity of recent data in the country and its description in neighboring countries. For this purpose, livers were collected from layer chicks (n=25), older layers (n=25), broilers (n=300), and livers (n=25) and stool (n=25) samples from broiler breeders, representing the major poultry regions of the state. FAdV DNA was demonstrated using a previously described PCR protocol for amplifying part of the hexon gene encoding sequence. FAdV was found in layer chicks (36%), widespread (100%) in older layers, and with regional differences in broilers (24-86%). Although all broiler breeder stools were negative, FAdV DNA was detected in livers (16%, 4/25) of stool-negative birds. In order to obtain additional information on the circulation of the infection, livers of subsistence chickens collected from one poultry intensive region, were evaluated (n = 12), with FAdV being detected in all samples. FAdV was found in young and old layers, broilers, broiler breeders and free-range chickens, and results suggest the circulation of FAdV among different types of chickens. The detection in older layer chickens may indicate an extended risk of horizontal transmission in regions of Minas Gerais with mixed activity of egg and meat type chickens and poor biosecurity strategies. The infection in breeders may indicate vertical transmission and the continuous production of infected progenies. The hexon-gene-targeted PCR amplicon sequences aligned with FAdV of species D of Aviadenovirus. Results indicate the necessity for biosecurity, especially for breeders, separating flocks according to origin, age and health status, which will be an advantage regarding any pathogen.Keywords: Aviadenovirus, FAdV, layer chicken, broiler chicken, broiler breeder RESUMO Descreve-se a ocorrência de Aviadenovirus (FAdV) na avicultura mineira. Foram amostrados fígados de poedeiras jovens (n=25) e velhas (n=25) e de frangos de corte (n=300). Em matrizes pesadas foram amostrados fígados (n=25) e fezes (n=25). O estudo envolveu as principais regiões avícolas do Estado de

Identification of four serotypes of fowl adenovirus in clinically affected commercial poultry co‐infected with chicken infectious anaemia virus in Trinidad and Tobago

“…Serological detections have been reported in Cuba, Guyana and Trinidad & Tobago and molecular detection of the virus has been reported in the Dominica Republic (Brown Jordan, Gongora, Hartley, & Oura, 2018;Brown Jordan, Sookhoo, et al, 2018;Schachner, Marek, Grafl, & Hess, 2016). There have however been a number of reports of FAdV being present and causing disease in poultry from the nearby territories of Latin America, including the presence of FAdV-4 (Hafez, 2011;Liu et al, 2016;Mazaheri et al, 1998;Pereira et al, 2014;Rodríguez, Koga, Alvarado, & Tinoco, 2014;Toro et al, 1999). A recent publication on the molecular characterization of disease causing FAdV strains from Brazil revealed the presence of FAdV-8a, FAdV-8b and FAdV-11 (De la Torre, Nunez, Parra, Astolfi-Ferreira, & Ferreira, 2018).…”

Polyostotic osteosarcoma associated with avian leukosis virus infection in a captive bare‐faced curassow (Crax fasciolata)

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Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific-pathogen-free chicken

“…Globally, the circulation of FAdV is widespread, covering most geographical locations, and it appears to infect not only chickens but also various other avian species [ 3 ]. A feature of FAdV epidemiology is the unusually large number of serotypes that can be isolated on a single farm [ 4 ]. It is also common to isolate multiple FAdV serotypes from the same diseased bird, suggesting a lack of cross-protection among serotypes [ 3 ].…”

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