Occurrence of bacteria species responsible for vibriosis in shrimp pond culture systems in Sri Lanka and assessment of the suitable control measures

Abstract

Shrimp culture in Sri Lanka is continuously affected by bacterial infections and most symptoms agree with the disease called vibriosis. Therefore a bacteriological study was undertaken in shrimp farms to identify the diversity of pathogenic Vibrio species, favorable environmental conditions for them and sensitivity to antibiotics to control them.Water and infected animals from shrimp farms of the Western and North Western provinces were screened for Vibrio. Conventional methods were used for presumptive identification of isolated bacteria. Water quality was analyzed by spectophotometric methods. Disk diffusion technique was used to evaluate antibiotic sensitivity. During the study, 40 bacteria isolates belonging to the family Vibrionaceae were recorded. Of those, 24 isolates belonged to Vibrio species which are responsible for vibriosis in shrimps. Those were Vibrio alginolyticus, V. parahaemolyticus, V. damsela, and V. anguillarum. Most frequently isolated species was V. parahaemolyticus. All Vibrio isolates were found to be resistant to Ampicillin, Gentamycin, Oxytetracyclin, Chloramphenicol, Trimethoprim and Kanamicin, which are commonly used antibiotics in aquaculture. Water quality conditions indicated that the alkaline pH conditions and high salinity and ammonia levels favour the occurrence of high vibrio counts.Results indicate that the application of antibiotics for the control of vibriosis in shrimp farms has limited effectiveness due to the development of resistant bacterial strains and environmental management is the best management intervention that can be used to control this disease.

Acreditation Certificate Acreditation No. 21/E/KPT/2018 Article Tools Print this article Indexing metadata How to cite item Supplementary files Email this article Email the author About The Authors Heri Ariadi ORCID iD http://ub.edu/heri Fisheries and Marine Science Faculty, University of Brawijaya Indonesia Good luck to RJLS Publisher Mohammad Mahmudi Fisheries and Marine Science Faculty, University of Brawijaya Indonesia Mohamad Fadjar Fisheries and Marine Science Faculty, University of Brawijaya Ind

“…The high concentration of organic matter and the density of vibrio bacteria that are pathogenic in pond ecosystems is an indication of the accumulation of waste loads due to aquaculture activities, which are usually caused by increased feed inputs, phytoplankton blooms, poor water quality management practice and unsupportive to environmental factors (Lekshmi et al, 2014). Vibrio bacteria that are sensitive to environmental conditions will be very opportunistic if their abundance is above the threshold (Heenatigala and Fernando, 2016). So that, it will cause various multiple diseases in cultivated shrimp such as white feces syndrome, Enterocytozoon hepatopenaei (EHP) infection, and mass death caused by physiological symptoms caused by Vibrio bacterial infections (Jayasree et al, 2006;Sriurairatana et al, 2014;Mastan, 2015;and Aranguren et al, 2017).…”

Vibrio spp . COMO PATÓGENOS NA CARCINICULTURA: ALTERNATIVAS DE CONTROLE

“…Devido a ineficiência do uso de antibióticos no combate às vibrioses na carcinicultura, a manutenção e gestão ambiental são consideradas como melhores ferramentas de intervenção no controle dessas doenças (Heenatigala & Fernando, 2016). Prevenir e controlar as doenças em camarão de viveiro é uma prioridade para o crescimento e sustentabilidade dessa indústria.…”

Aktivitas Antibakteri Isolat Bakteri Asam Laktat Intestinal Udang Penaeid Tipe Liar Terhadap Bakteri Vibrio

“…(Shakila et al, 2006), udang Vaname hasil budidaya (Vieira et al, 2013), ikan dan udang (Noordiana et al, 2013), kuruma shrimp (Marsupenaeus japonicus) (Maeda et al, 2014), Fenneropenaeus chinensis dan ikan Acanthogobius hasta (Sha et al, 2016 Aktivitas antibakteri merupakan salah satu karakter utama probiotik untuk mampu memproteksi inang dari infeskai pathogen didalam saluran cernanya. Salah satu kemampuan penghambatan pathogen adalah melalui produksi metabolit seperti asam organik (asam laktat dan asam asetat) (Suskovic et al, 2010) -Rodriguez et al, 2010 ;Heenatigala & Fernando, 2016 ;Hong et al, 2016). Vibrio juga diketahui sebagai agensia penyebab penyakit early mortality syndrome (EMS) atau "acute hepatopancreatic necrosis disease" (AHPND) (De Schryver et al, 2014).…”

“…Kata Kunci : bakteri asam laktat, antibakteri, vibrio, udang penaeid PENDAHULUAN Vibrio merupakan salah satu agensia penyebab penyakit pada udang. Jenisjenis penyakit vibriosis ini meliputi black shell disease, tail rot, septic hepatopancreatic necrosis, brown gill disease, swollen hindgut syndrome dan luminous bacterial disease (Heenatigala & Fernando, 2016). Jenis penyakit yang disebabkan oleh Vibrio yang saat ini merupakan penyakit baru dan ganas adalah early mortality syndrome (EMS) atau acute hepatopancreatic necrosis disease (AHPND).…”