Occurrence of both nonvolatile and semivolatile carbonaceous air particulate markers using thermal desorption-pyrolysis-gas chromatography-mass spectrometry

Nespor, Brett; Cochran, Richard E.; Jeong, H.; Bowman, Frank M.; Delene, David J.; Kozliak, Evguenii; Kubátová, Alena

doi:10.1016/j.atmosenv.2020.118058

Cited by 8 publications

(10 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OC and EC content of tunnel PM samples were analyzed by a method adopted from NIOSH temperature protocol allowing to distinguish different temperature fractions (i.e., volatility and molecular weight as previously described [75][76][77] using thermal optical analyzer (Sunset Laboratory, Inc.). For analysis, 5 µL aliquots of PM solutions (10 mg/ml) were placed onto pre-baked quartz filters (600 °C, overnight) and dried on a hot plate set to 50 °C for 7 min.…”

Section: Oc and Ec Analysesmentioning

confidence: 99%

Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Traffic particulate matter (PM) comprises a mixture of particles from fuel combustion and wear of road pavement, tires and brakes. In countries with low winter temperatures the relative contribution of mineral-rich PM from road abrasion may be especially high due to use of studded tires during winter season. The aim of the present study was to sample and characterize size-fractioned PM from two road tunnels paved with different stone materials in the asphalt, and to compare the pro-inflammatory potential of these fractions in human bronchial epithelial cells (HBEC3-KT) in relation to physicochemical characteristics. Methods The road tunnel PM was collected with a vacuum pump and a high-volume cascade impactor sampler. PM was sampled during winter, both during humid and dry road surface conditions, and before and after cleaning the tunnels. Samples were analysed for hydrodynamic size distribution, content of elemental carbon (EC), organic carbon (OC) and endotoxin, and the capacity for acellular generation of reactive oxygen species. Cytotoxicity and pro-inflammatory responses were assessed in HBEC3-KT cells after exposure to coarse (2.5–10 μm), fine (0.18–2.5 μm) and ultrafine PM (≤ 0.18 μm), as well as particles from the respective stone materials used in the pavement. Results The pro-inflammatory potency of the PM samples varied between road tunnels and size fractions, but showed more marked responses than for the stone materials used in asphalt of the respective tunnels. In particular, fine samples showed significant increases as low as 25 µg/mL (2.6 µg/cm2) and were more potent than coarse samples, while ultrafine samples showed more variable responses between tunnels, sampling conditions and endpoints. The most marked responses were observed for fine PM sampled during humid road surface conditions. Linear correlation analysis showed that particle-induced cytokine responses were correlated to OC levels, while no correlations were observed for other PM characteristics. Conclusions The pro-inflammatory potential of fine road tunnel PM sampled during winter season was high compared to coarse PM. The differences between the PM-induced cytokine responses were not related to stone materials in the asphalt. However, the ratio of OC to total PM mass was associated with the pro-inflammatory potential.

show abstract

Section: Oc and Ec Analysesmentioning

confidence: 99%

Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although the as-received filters are suitable for other types of analyses, the ubiquity of microplastics necessitates filter conditioning. Conditioning at an elevated temperature, preferably at or above the pyrolysis temperature for 8 to 24 h removes microplastic contamination from the filter. ,, The importance of conditioning the filters prior to sampling is well illustrated in Figure , which shows the EGA thermogram of the quartz fiber filters used. Significant contamination was eliminated by conditioning the filters.…”

Section: Resultsmentioning

confidence: 99%

“…As a result of this study filters are stored in sealed, clean metal containers inside an aluminum lined bag and are kept in a refrigerator to limit the effects of thermal cycling. 49 Filters are exposed to laboratory air briefly and only when necessary. The sensitivity of F-Splitless Py-GC/MS, which has the capacity to load all the pyrolyzates onto the analysis system, requires that extra care and caution be taken when handling samples, even "blank" samples.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…small-organic molecules must be addressed; whether by digestion, 47 solvent or acid 50 treatment or thermal extraction. 45,49 Studies have shown the utility of thermal extraction clean-up in diverse matrices such as sand, biosolids, rice and blood. 81−84 Therefore, we decided to analyze the PM 2.5 portion of the active sample using both DS and SS for comparison.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Into the Nanograms─Sensitive Detection of Microplastics in Passively Sampled Indoor Air Using F-Splitless Pyrolysis Gas Chromatography Mass Spectrometry

Pipkin,

Sato,

Kumagai

et al. 2024

ACS EST Air

View full text Add to dashboard Cite

The presence in the atmosphere of airborne plastic, referred to as microplastic, results from the large quantities of plastic waste produced by humans. Microplastics can have an impact on human health. In this study, we investigated the analysis of indoor airborne microplastics by pyrolysis-gas chromatography/ mass spectrometry (Py-GC/MS). Py-GC/MS can be used to identify and quantify various types of microplastics. Indoor air was passively sampled using quartz fiber filters. In one instance, active sampling was used for comparative purposes. Sample preparation was limited and consisted only of cryomilling the quartz filters used for sampling to ensure homogeneity. F-Splitless pyrolysis, which is accomplished by forcibly redirecting flows, was used to transfer all the pyrolyzates of the microplastics to the GC/MS system. The results demonstrate that F-Splitless Py-GC/MS can be used to identify and quantify microplastics at the nanogram level. This method enables the rapid characterization and quantitation of microplastics in particulate matter (PM) air filters with minimal sample preparation, direct measurement of the sampling media, and nanogram-level sensitivity, thereby, allowing researchers to ascertain the magnitude of the microplastic threat in environmental air.

show abstract

“…With the increase in temperature, a shift toward the thermal fragment ions CO + ( m / z 28) and CO 2 + ( m / z 44) is observed in the mass spectra. However, although thermal decomposition of highly oxidized species can be observed already at temperatures below 100 °C, a certain part of the non-volatile sample matrix, such as extremely low volatility organic compounds (ELVOCs), can only be targeted by thermal decomposition at temperatures higher than 400 °C . Because the maximum temperature of DIP–HRTOFMS is capped at 400 °C, complete pyrolysis cannot be guaranteed.…”

Section: Resultsmentioning

confidence: 99%

On the Complementarity and Informative Value of Different Electron Ionization Mass Spectrometric Techniques for the Chemical Analysis of Secondary Organic Aerosols

Hartner

Paul

Käfer

et al. 2022

ACS Earth Space Chem.

View full text Add to dashboard Cite

The atmospheric aging of volatile organic compounds leads to the formation of complex mixtures of highly oxidized secondary organic aerosols (SOAs). State-of-the-art mass spectrometry (MS) has become a pivotal tool for their chemical characterization. In this study, we characterized the chemical complexity of naphthalene-derived SOA by three different time-of-flight (TOF) mass spectrometric techniques applying electron ionization: high-resolution–TOF–aerosol MS (AMS), direct inlet probe (DIP)–high-resolution TOFMS, and thermal desorptioncomprehensive two-dimensional gas chromatographyTOFMS (GC × GC). We discuss AMS as an online, DIP as an atline, and GC × GC as an offline technique to compare their informative value for studying the oxidation state, volatility, and molecular composition of laboratory-generated SOA. For GC × GC, the accessible organic content was limited to (semi-)volatile compounds and supported a reliable assignment of the molecular composition. DIP and AMS were used to derive secondary parameters such as O/C and H/C ratios, the general functionality of the compound classes and their abundance upon photochemical aging. Thereby, while the induced pyrolysis in the AMS extended the accessibility range to polar, high-molecular-weight compounds, thermal fragmentation also led to limited molecular information. For DIP, low-volatility compounds could be volatilized and the high mass resolution was useful to resolve isobaric mass fragments and assign reliable sum formulas of fragments and molecular ions. Although no single technique can provide information to describe the full chemical complexity of the SOA, AMS, DIP, and GC × GC in their complementarity are well suited to investigate the impact of SOA on health and environment.

show abstract

Occurrence of both nonvolatile and semivolatile carbonaceous air particulate markers using thermal desorption-pyrolysis-gas chromatography-mass spectrometry

Cited by 8 publications

References 95 publications

Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells

Road tunnel-derived coarse, fine and ultrafine particulate matter: physical and chemical characterization and pro-inflammatory responses in human bronchial epithelial cells

Into the Nanograms─Sensitive Detection of Microplastics in Passively Sampled Indoor Air Using F-Splitless Pyrolysis Gas Chromatography Mass Spectrometry

On the Complementarity and Informative Value of Different Electron Ionization Mass Spectrometric Techniques for the Chemical Analysis of Secondary Organic Aerosols

Contact Info

Product

Resources

About