Occurrence of Campylobacter in retail meat in Lahore, Pakistan

Nisar, Muhammad Imran; Ahmad, Mansur ud Din; Mushtaq, Muhammad; Shehzad, Wasim; Hussain, Abid; Nasar, Mohammad; Nagaraja, K. V.; Goyal, Sagar M.

doi:10.1016/j.actatropica.2018.04.030

Cited by 33 publications

(23 citation statements)

References 18 publications

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“…In this study, the prevalence rate of Campylobacter spp. in chicken meat was 10.3% (31/300) classified into C. jejuni 64.5% (20/31) and C. coli 35.48% (11/31) which was in agreement with Nisar et al (2018). All C. jejuni isolates were confirmed by real time PCR (Figure 2).…”

Section: Resultssupporting

confidence: 73%

Prevalence, cytotoxicity and antibiotic susceptibility of Campylobacter species recovered from retail chicken meat in Mansoura, Egypt

Hafez¹,

Younis²,

El-Shorbagy³

et al. 2018

Afr. J. Microbiol. Res.

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This study was performed to determine the prevalence of Campylobacter species in retail chicken meat and chicken by-product, determine their in vitro cytotoxicity, as well as, examine their susceptibility to different antimicrobials. A total of 300 raw chicken meat samples were collected from different retail chicken meat outlets located at Mansoura city, Egypt classified into 120 thighs, 120 breasts, and 60 livers. All samples were subjected to conventional culture techniques and confirmed as Campylobacter jejuni by real time polymerase chain reaction (PCR). Antimicrobial susceptibility of Campylobacter species was determined using disc diffusion method to determine their susceptibility to 12 different antimicrobial agents. In addition, C. jejuni isolates were examined for their cytotoxicity against Vero cells. The overall prevalence of Campylobacter spp. was 10.3% (31/300) classified into 20 (18.2%) C. jejuni and 11 (10.7%) Campylobacter coli. Among C. jejuni isolates (n=20), 15 strains belonged to biotype I and 5 isolates belonged to biotype II. The isolation rate from chicken thighs, breasts and livers was 12.5, 10 and 6.6%, respectively. A total of 15 (75%) C. jejuni strains revealed cytopathic effect (CPE) against Vero cells. Campylobacter spp. displayed a high antimicrobial resistance against penicillin G, gentamicin, trimethoprim-sulfamethoxazole, cephalothin, erythromycin, and chloramphenicol. On the other hand, Campylobacter spp. displayed high sensitivity to ciprofloxacin and nalidixic acid. Multidrug resistance was observed in 85 and 81.82% of C. jejuni and C. coli isolates, respectively. High frequency of cytotoxicity and multidrug resistance in Campylobacter spp. from chicken meat indicates an important epidemiological role of Campylobacter spp. in human infections which necessitate proper hygienic measures on poultry farms and control measures during carcass slaughtering and processing.

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Section: Resultssupporting

confidence: 73%

Prevalence, cytotoxicity and antibiotic susceptibility of Campylobacter species recovered from retail chicken meat in Mansoura, Egypt

Hafez¹,

Younis²,

El-Shorbagy³

et al. 2018

Afr. J. Microbiol. Res.

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“…Studies carried out in Italy by Stella et al [55], in Malaysia [56], in China [57], in France [58], and in South Korea [59] also detected Campylobacter in meat samples with detection rates of 34.10%, 50.9%, 48.9%, 76%, and 31.67%, respectively, and our findings are in line with their reports. Other studies carried out in Spain by García-Sánchez et al [60], in Pakistan by Nisar et al [3], in Yangzhou, China by Zou et al [61], and in Northern Poland by Andrzejewska et al [62] also detected Campylobacter in meat samples, and these results are also akin with their reports. According to Seliwiorstow et al [63], different sampling sources make an impact on the occurrence rates of Campylobacter, which indicate the risk factors associated with handling.…”

Section: Discussionsupporting

confidence: 71%

“…In the last decade, there has been a global upsurge in the rate of Campylobacter infections [1,2], and Campylobacter has emerged as one of the most significant bacteria of public health importance [3]. Globally, Campylobacter infection is a significant zoonosis, considered to be the leading cause of bacterial foodborne infection [4].…”

Section: Introductionmentioning

confidence: 99%

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

Igwaran

Okoh

2020

Foods

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Raw meats are sometimes contaminated with Campylobacter species from animal faeces, and meats have repeatedly been implicated in foodborne infections. This study evaluated the prevalence, virulence genes, antimicrobial susceptibility patterns, and resistance gene determinants in Campylobacter species isolated from retailed meat carcasses. A total of 248 raw meat samples were collected from butcheries, supermarkets, and open markets; processed for enrichment in Bolton broth; and incubated at 42 °C for 48 h in 10% CO2. Thereafter, the broths were streaked on modified charcoal cefoperazone deoxycholate agar (mCCDA) plates and incubated at the same conditions and for the same amount of time. After incubation, colonies were isolated and confirmed by Polymerase chain reaction using specific oligonucleotide sequences used for the identification of the genus Campylobacter, species, and their virulence markers. The patterns of antimicrobial resistance profiles of the identified isolates were studied by disk diffusion method against 12 antibiotics, and relevant resistance genes were assessed by PCR. From culture, 845 presumptive Campylobacter isolates were obtained, of which 240 (28.4%) were identified as genus Campylobacter. These were then characterised into four species, of which C. coli had the highest prevalence rate (22.08%), followed by C. jejuni (16.66%) and C. fetus (3.73%). The virulence genes detected included iam (43.14%), cadF (37.25%), cdtB (23.53%), flgR (18.63%), and flaA (1.96%), and some of the isolates co-harboured two to four virulence genes. Of the 12 antibiotics tested, the highest phenotypic resistance displayed by Campylobacter isolates was against clindamycin (100%), and the lowest level of resistance was observed against imipenem (23.33%). The frequency of resistance genes detected included catll (91.78%), tetA (68.82%), gyra (61.76%), ampC (55%), aac(3)-IIa (aacC2)a (40.98%), tetM (38.71%), ermB (18.29%), tetB (12.90%), and tetK (2.15%). There is a high incidence of Campylobacter species in meat carcasses, suggesting these to be a reservoir of campylobacteriosis agents in this community, and as such, consumption of undercooked meats in this community is a potential health risk to consumers.

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“…The overall positivity status estimated under this study has been supported by other research both in home and abroad. A positive status of 32% Campylobacter in broiler flocks was found in India [35], and 29% and 21.5% in Pakistan [36,37]. However, Campylobacter occurrence in broiler samples was confirmed as 32% and 40.5% in Bangladesh [20,21].…”

Section: Discussionmentioning

confidence: 90%

Occurrence of Campylobacter spp. in Selected Small Scale Commercial Broiler Farms of Bangladesh Related to Good Farm Practices

et al. 2020

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Poultry origin Campylobacter is considered as one of the leading causal agents of human foodborne illness. This study was conducted to estimate the occurrence, molecular identification, and antimicrobial resistance (AMR) of Campylobacter species from the broiler farms in Bangladesh. Samples (352) were collected from 32 farms and comprised of 128 cloacal swab, 64 feed, 64 drinking water, 64 attendants’ hand rinsed water, and 32 whole carcasses. All samples were tested for the presence of Campylobacter via cultural, biochemical, and PCR. The AMR was determined via the disc diffusion method. An overall occurrence of Campylobacter spp. was estimated as 26.4%. The level of Campylobacter contamination was found to be higher in conventional farms (36.4%) than the good practice farms (16.5%) including all sample categories (p = 0.000). Of 93 isolates, 67.74% and 32.26% were confirmed as C. jejuni and C. coli respectively, of which 34.92% C. jejuni, and 30% C. coli were shown to be multidrug-resistant. A significant occurrence of Campylobacter contamination in broiler farms with multidrug resistant patterns might be cogitated as serious food safety and public health concern linking to poultry food chain. A risk reduction approach through good farming practices targeting the prudent use of antimicrobials for broiler production is thus necessitated.

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Occurrence of Campylobacter in retail meat in Lahore, Pakistan

Cited by 33 publications

References 18 publications

Prevalence, cytotoxicity and antibiotic susceptibility of Campylobacter species recovered from retail chicken meat in Mansoura, Egypt

Prevalence, cytotoxicity and antibiotic susceptibility of Campylobacter species recovered from retail chicken meat in Mansoura, Egypt

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

Occurrence of Campylobacter spp. in Selected Small Scale Commercial Broiler Farms of Bangladesh Related to Good Farm Practices

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