Occurrence of cGMP/nitric oxide-sensitive store-operated calcium entry in fibroblasts and its effect on matrix metalloproteinase secretion

Huang, Yong; Lu, Min-qiang; Li, Hua; Xu, Chi; Yi, Shuhong; Chen, Guihua

doi:10.3748/wjg.v12.i34.5483

Cited by 11 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 3 to 4 cycles of the freezing and thawing procedure, the material was dehydrated in a lyophilizer at a temperature above −20°C and then sterilized by epoxy ethane. The result was a decellularized aorta . The following example demonstrates the effectiveness of SDS as a stand‐alone cell removal agent.…”

Section: Decellularization Agentsmentioning

confidence: 92%

“…The result was a decellularized aorta. 117 The following example demonstrates the effectiveness of SDS as a stand-alone cell removal agent. As a result of a 72-hour incubation of the porcine heart valve in 0.03-0.1% SDS at room temperature, a completely cell-free tissue was obtained.…”

Section: Chemical Decellularization Methodsmentioning

confidence: 97%

“…The positive effect of such mixed methods may be visible, for example, in full (for the mixed method) and partial (for the single‐step method) removal of cell nuclei from the tissue . Satisfactory results of the stirred decellularization procedure, which assumes the use of trypsin and SDS, are confirmed by the studies of Huang et al A study was performed in which approximately 10 cm long sections of porcine thoracic aorta were incubated in a 0.025% trypsin solution for 24 hours at 37°C. The material obtained in this way was then subjected to a 0.5% SDS solution under continuous shaking.…”

Section: Decellularization Agentsmentioning

confidence: 95%

See 2 more Smart Citations

A review of decellurization methods caused by an urgent need for quality control of cell‐free extracellular matrix' scaffolds and their role in regenerative medicine

et al. 2017

View full text Add to dashboard Cite

The natural extracellular matrix (ECM),thanks to its specific properties (e.g., collagenous lattice, a reservoir of growth factors, ECM-cell anchoring areas, an optimal pH and CO ),ensures an optimal microenvironment for homeostatic and regenerative cell development. In the context of regenerative medicine, ECM is a lair for residual and infiltrative cells. The aim of the clinical usage of cell-free ECM scaffolds is the enhancement of tissue regeneration with possible minimization of an adverse host reaction on allogeneic or xenogeneic biomaterial. Thus, the objective of decellularization is to obtain acellular grafts characterized by optimal biological properties, such as a lack of remaining cellular elements (e.g., cell membrane phospholipids and proteins, nucleic acids, mitochondria), lack of immunogenicity, lack of calcification promotion and lack of cytotoxicity (e.g., in unrinsed detergents). Furthermore, cell-free ECM scaffolds should present the optimal mechanical and structural properties that may ensure the biocompatibility of the graft. The maintenance of the ultrastructure composition of the ECM is one of the most important goals of decellularization. All physical, chemical, and biological methods proposed (used separately or in combination to extract cells from tissues/organs) are not 100% effective in cell removal and always cause a disruption of the ECM texture, as well as a probable loss of important structure components. Although cell-free ECM scaffolds are generally classified as medical devices, there are no widely accepted or legally defined criteria for quality control/evaluation methods of obtained matrices. Such criteria must be provided. Some of them have been proposed in this manuscript. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 909-923, 2018.

show abstract

Section: Decellularization Agentsmentioning

confidence: 92%

Section: Chemical Decellularization Methodsmentioning

confidence: 97%

Section: Decellularization Agentsmentioning

confidence: 95%

See 1 more Smart Citation

A review of decellurization methods caused by an urgent need for quality control of cell‐free extracellular matrix' scaffolds and their role in regenerative medicine

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In mouse fibroblasts, increasing [Ca 2+ ] e induces release and activation of MMP-2 and -9. The MMPs release could be dependent on store-operated Ca 2+ channels and the NO/cGMP/PKG pathway negatively regulates this Ca 2+ entry pathway (Huang et al, 2006). Also, PAF induces MMP-9 expression in human vascular ECs through Ca 2+ - or PI 3 K-dependent signaling pathway (Ko et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Vascular and Cellular Calcium in Normal and Hypertensive Pregnancy

Adamová¹,

Ozkan²,

Khalil³

2009

CCP

View full text Add to dashboard Cite

Normal pregnancy is associated with significant hemodynamic changes in the cardiovascular system in order to meet the metabolic demands of mother and fetus. These changes include increased cardiac output, decreased vascular resistance, and vascular remodeling in the uterine and systemic circulation. Preeclampsia (PE) is a major complication of pregnancy characterized by proteinuria and hypertension. Several risk factors have been implicated in the pathogenesis of PE including genetic and dietary factors. Ca 2+ is an essential dietary element and an important regulator of many cellular processes including vascular function. The importance of adequate dietary Ca 2+ intake during pregnancy is supported by many studies. Pregnancy-associated changes in Ca 2+ metabolism and plasma Ca 2+ have been observed. During pregnancy, changes in intracellular free Ca 2+ concentration ([Ca 2+ ] i ) have been described in red blood cells, platelets and immune cells. Also, during pregnancy, an increase in [Ca 2+ ] i in endothelial cells (EC) stimulates the production of vasodilator substances such as nitric oxide and prostacyclin. Normal pregnancy is also associated with decreased vascular smooth muscle (VSM) [Ca 2+ ] i and possibly the Ca 2+ -sensitization pathways of VSM contraction including protein kinase C, Rho-kinase, and mitogen-activated protein kinase. Ca 2+ -dependent matrix metalloproteinases could also promote extracellular matrix degradation and vascular remodeling during pregnancy. Disruption in the balance between dietary, plasma and vascular cell Ca 2+ may be responsible for some of the manifestation of PE including procoagulation, decreased vasodilation, and increased vasoconstriction and vascular resistance. The potential benefits of Ca 2+ supplements during pregnancy, and the use of modulators of vascular Ca 2+ to reduce the manifestations of PE in susceptible women remain an important area for experimental and clinical research.

show abstract

“…Translocation of STIM puncta and STIM/Orai binding; Partially Orai channel DeHaven et al, 2008;Gregory et al, 2001;Iwasaki et al, 2001;Peinelt et al, 2008 D. Pyrazole compounds: Bis(trifluoromethyl) (BTP1, BTP2 and BTP3) SOCE Sweeney et al, 2009 E. ML-9 Myosin light chain kinase (MLCK) Coalescence of STIM1 into puncta Smyth et al, 2008 F. Diethylstilbestrol (DES 18) -a synthetic estrogen agonist SOCE in mast cells and vascular smooth muscle cells Hoth and Penner, 1993;Ohana et al, 2009 G. Carboxyamidotriazole (CAI) Ca 2+ dependent inactivation CRAC Rodland et al, 1997 H. RO2959 Human TCR mediated SOCE Chen et al, 2013 I. Linoleic acid: 18-C polyunsaturated fatty acid (PUFA) SOCE by affecting STIM1 oligomerization and subsequent STIM1/ORAI1 coupling Holowka et al, 2014 diomyocytes (Huang et al, 2006;Touchberry et al, 2011). Different molecules of SOCC including Orai 1, Orai 3 and STIM1 were found to regulate normal and hypertrophic growth in cardiomyocytes (Ohba et al, 2009;Saliba et al, 2015;Voelkers et al, 2010).…”

Section: Soce Inhibitorsmentioning

confidence: 99%

Store-operated calcium channels: Potential target for the therapy of hypertension

Sukhwinder

Anureet

Naranjan

2019

Rev. Cardiovasc. Med.

View full text Add to dashboard Cite

Effective therapy of hypertension represents a key strategy for reducing the burden of cardiovascular disease and its associated mortality. The significance of voltage dependent L-type Ca 2+ channels to Ca 2+ influx, and of their regulatory mechanisms in the development of heart disease, is well established. A wide variety of L-type Ca 2+ channel inhibitors and Ca 2+ antagonists have been found to be beneficial not only in the treatment of hypertension, but also in myocardial infarction and heart failure. Over the past two decades, another class of Ca 2+ channel-the voltage independent store-operated Ca 2+ channel-has been implicated in the regulation and fine tuning of Ca 2+ entry in both cardiac and smooth muscle cells. Storeoperated Ca 2+ channels are activated by the depletion of Ca 2+ stores within the endoplasmic/sarcoplasmic reticulum, or by low levels of cytosolic Ca 2+ , thereby facilitating agonist-induced Ca 2+ influx. Store-operated Ca 2+ entry through this pivotal pathway involves both stromal interaction molecule (STIM) and Orai channels. Different degrees of changes in these proteins are considered to promote Ca 2+ entry and hence contribute to the pathogenesis of cardiovascular dysfunction. Several blockers of storeoperated Ca 2+ channels acting at the level of both STIM and Orai channels have been shown to depress Ca 2+ influx and lower blood pressure. However, their specificity, safety, and clinical significance remain to be established. Thus, there is an ongoing challenge in the development of selective inhibitors of store-operated Ca 2+ channels that act in vascular smooth muscles for the improved treatment of hypertension.

show abstract

Occurrence of cGMP/nitric oxide-sensitive store-operated calcium entry in fibroblasts and its effect on matrix metalloproteinase secretion

Abstract: NO/cGMP sensitive store-operated Ca(2+) entry occurs in fibroblasts, and attenuates their adhesion potentials through its influence on MMP secretion.

Cited by 11 publications

References 34 publications

A review of decellurization methods caused by an urgent need for quality control of cell‐free extracellular matrix' scaffolds and their role in regenerative medicine

A review of decellurization methods caused by an urgent need for quality control of cell‐free extracellular matrix' scaffolds and their role in regenerative medicine

Vascular and Cellular Calcium in Normal and Hypertensive Pregnancy

Store-operated calcium channels: Potential target for the therapy of hypertension

Contact Info

Product

Resources

About