Occurrence of Coagulase Serotype among Staphylococcus aureus Strains Isolated from Healthy Individuals —Special Reference to Correlation with Size of Protein‐A Gene—

Abstract

One-hundred-and-nineteen strains of Staphylococcus aureus isolated from healthy individuals for 3 years between 1991 and 1993 were subjected to an investigation on the producibility of proteins including protein A, coagulase, enterotoxins and toxic-shock syndrome toxin-1. Especially, protein A was the center of our interest. Among these strains, 69, 43, 3 and 1 strains were found to have the protein-A gene containing 5, 4, 3 and 2 IgG-binding domains, respectively. On the other hand, only one strain was devoid of the protein-A gene. There were some differences in the profile of the coagulase serotype between the group with 4 IgG-binding domains and that with 5 IgG-binding domains. Differences in the profile of toxin production were also observed between the two groups.

Staphylococcus argenteus from rabbits in Thailand

“…In S. argenteus isolates, further identification of the virulence genes was performed, including classical enterotoxin ( sea , seb , sec , sed, and see ) (Wu et al., ), hemolysin ( hla and hlb ) (Jarraud et al., ), clumping factor ( clf A) (Tristan et al., ), protein A [ spa x region (Frénay et al, ) and spa IgG‐binding region (Seki et al, )], coagulase ( coa ) (Aslantas et al., ), and Panton‐Valentine leukocidin ( pvl ) (Jarraud et al., ), with specific oligonucleotide primers. PCR reactions were performed, involving initial denaturation at 95°C for 10 min; 35 cycles of 95°C for 30 s, 55°C for 30 s, and 72°C for 30 s and a final extension at 72°C for 10 min.…”

IgG-dependent aggregation of Staphylococcus aureus inhibits bacteriophage attack

“…This protein usually consists of four to five highly homologous IgG-binding domains, followed by a putative cell wall-spanning X-region with an LPXTG-motif that attaches to the peptidoglycan layer in the cell wall [24]. The X region and the IgG-binding domain of the protein A gene (spa) of SA003 have been identified [17].…”

Characterization of Genes Encoding Virulence Determinants and Toxins in Staphylococcus aureus from Bovine Milk in Central Italy

“…The genetic determinants for the following virulence traits were investigated using oligonucleotide primers derived from the published sequences: this included the genes encoding clumping factor (clfA) [34], X-region [16] and IgG binding-region of protein A (spa) [29], SEs: sea [37], seb, sec, sed, see [21], seg, seh, sei [19] and sej [25], thermonuclease (nuc) [8], fibronectin binding protein A (fnbA) and fibronectin binding protein B (fnbB) [6] and capsular polysaccharide 5 (cap5) and 8 (cap8) [26]. The amplified products were sequenced.…”