Occurrence of Crown Gall of Rose and Rose Cultivar-specific Resistance

Han, Kyung Sook; Kim, Wonhee; Park, Jong Han; Lee, Jung Sup; Seo, Sang‐Tae

doi:10.5423/rpd.2006.12.2.075

Cited by 1 publication

(3 citation statements)

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“…Callus tissue can be formed on stem explants in vitro. It is a common phenomenon in an in vitro culture (Han et al, 2006;Hsia and Korban, 1996;Khosh-Khui and Sink, 1982). In the present study, callus formation was observed in 32 cultivars.…”

Section: Resultssupporting

confidence: 49%

“…In order to investigate the susceptibility of Korean and foreign rose cultivars to A. tumefaciens, the selected pathogenic strain RC12 strain was streaked onto yeast extract beef medium. After incubation at 25℃ for 48 h, bacterial suspension was adjusted to 10 8 cfu/ml and was prepared for inoculation (Han et al, 2006;Zhou et al, 2000). Rose stems were collected from 58 Korean cultivars and six foreign cultivars and sterilized with 75% ethanol for 2 min followed by treatment with 3% sodium hypochlorite containing Tween 20 (0.1%) for 10 min.…”

Section: Isolation Of Pathogenmentioning

confidence: 99%

“…In susceptible cultivars with tumor formation, the size of tumor increased over time with tumor surface color changed from white to brown. Han et al (2006) and Park and Cha (2000) have reported that after infection by A. tumefaciens, small tumors can develop with increasing sizes, and tumor surface color turning brown. Six cultivars 'Love Letter','Redbi',and 'Feel Lip') had tumor formation rate of 16% to 25%.…”

Section: Resistance Test Of Rose Cultivars Against Crown Gallmentioning

confidence: 99%

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Cultivar Resistance of Korean Breeding Cut-Rose against Crown Gall by Agrobacterium tumefaciens Evaluated by an In Vitro Inoculation

2023

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Rose crown gall caused by Agrobacterium tumefaciens is a major disease that damages the production of cut-roses in Korea. The effective prevention methods for this disease include the use of resistant varieties. This study was conducted to evaluate the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease with nodal explants in vitro. Among 180 A. tumefaciens strains, pathogenic strain RC12 was selected as an inoculant strain. The strain RC12 was identified based on characteristics of some selective media, pathogenicity test, and polymerase chain reaction analysis. Forty rose cultivars formed tumors on explants inoculated with A. tumefaciens RC12. However, 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to A. tumefaciens RC12 without forming any tumors. Six cultivars with tumor formation rates of over 30% formed initial tumors within 23 days after inoculation. Six cultivars with low tumor formation rates of around 5% formed initial tumors after 28 days of inoculation. It was found that gall formation rate was highly correlated with the initial gall formation period. Thus, the relationship between the period of gall formation and the rate of gall formation could be useful for assessing resistance to crown gall disease. In vitro inoculation methods could be used to evaluate resistance of cut-rose cultivars to crown gall diseases.

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Section: Resultssupporting

confidence: 49%

Section: Isolation Of Pathogenmentioning

confidence: 99%

Section: Resistance Test Of Rose Cultivars Against Crown Gallmentioning

confidence: 99%

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