Occurrence of Cu-ATPase in Dictyostelium: Possible Role in Resistance to Copper

Burlando, Bruno; Evangelisti, V.; Dondero, Francesco; Pons, G.; Camakaris, J.; Viarengo, Aldo

doi:10.1006/bbrc.2002.6463

Cited by 28 publications

(24 citation statements)

References 24 publications

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“…TPEN is capable of crossing natural and artificial membranes and chelating divalent metals preferentially. This feature makes it useful in controlling the concentration of metal ions in biochemical experiments [29,[42][43][44][45]. Regardless of the high affinity of TPEN for copper instead of for zinc, it is perplexing that TPEN failed to protect against the neuronal cytotoxicity of Cu 2+ in rat hippocampus, whereas it protected against the toxicity induced by Zn 2+ [46].…”

Section: Acyclic Amino Chelatorsmentioning

confidence: 99%

The significance of copper chelators in clinical and experimental application

Ding

Xie

Kang

2011

The Journal of Nutritional Biochemistry

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Section: Acyclic Amino Chelatorsmentioning

confidence: 99%

The significance of copper chelators in clinical and experimental application

Ding

Xie

Kang

2011

The Journal of Nutritional Biochemistry

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“…Our hypothesis is supported by the presence of the homologous copper transporter 1 (Ctr1) in macrophages and P80 in Dictyostelium discoideum and Acanthamoeba castellanii-proteins, both of which are involved in Cu + uptake upon phagocytosis. In addition, amoebae are known to contain P-type ATPases (German et al 2013;Burlando et al 2002), and similar to macrophages, at least one of these P-type ATPases in A. castellanii could be pumping Zn 2+ or Cu + into the phagosome of amoeba (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Hao

Lüthje

Qin

et al. 2015

Appl Microbiol Biotechnol

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The presence of metal resistance determinants in bacteria usually is attributed to geological or anthropogenic metal contamination in different environments or associated with the use of antimicrobial metals in human healthcare or in agriculture. While this is certainly true, we hypothesize that protozoan predation and macrophage killing are also responsible for selection of copper/zinc resistance genes in bacteria. In this review, we outline evidence supporting this hypothesis, as well as highlight the correlation between metal resistance and pathogenicity in bacteria. In addition, we introduce and characterize the Bcopper pathogenicity island^identified in Escherichia coli and Salmonella strains isolated from copper-and zinc-fed Danish pigs.

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“…Dictyostelium shows an apparent resistance to Zn, Ag, Al and Cu at the concentration tested. This ability of Dictyostelium can be related to its various detoxification systems, such as catalase, glutathione, superoxide dismutase [26], Cu-ATPase [27] and HSP [28]. However, Cd, Hg, diazinon and dicofol affect the fission rate and the cell ability to eat and differentiate.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Temperature Variation on the Sensitivity to Pesticides: a Study on the Slime Mould Dictyostelium discoideum (Protozoa)

Amaroli

2014

Microb Ecol

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Slime moulds live in agricultural ecosystems, where they play an important role in the soil fertilization and in the battle against crop pathogens. In an agricultural soil, the amoebae are exposed to different stress factors such as pesticides and weather conditions. The use of pesticides increased up from 0.49 kg per hectare in 1961 to 2 kg in 2004, and the global greenhouse gas emission has grown 70% between 1970 and 2004 leading to a global fluctuation of average surface temperature. Therefore, the European Directive 2009/128/EC has led to a new approach to agriculture, with the transition from an old concept based on high use of pesticides and fossil fuels to an agriculture aware of biodiversity and health issues. We studied the effects of temperature variations and pesticides on Dictyostelium discoideum. We measured the fission rate, the ability to differentiate and the markers of stress such as the activity and presence of pseudocholinesterase and the presence of heat shock protein 70. Our results highlight how the sensitivity to zinc, aluminium, silver, copper, cadmium, mercury, diazinon and dicofol changes for a 2 °C variation from nothing/low to critical. Our work suggests considering, in future regulations, about the use of pesticides as their toxic effect on non-target organisms is strongly influenced by climate temperatures. In addition, there is a need for a new consideration of the protozoa, which takes into account recent researches about the presence in this microorganism of classical neurotransmitters that, similar to those in animals, make protozoa an innocent target of neurotoxic pesticides in the battle against the pest crops.

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Occurrence of Cu-ATPase in Dictyostelium: Possible Role in Resistance to Copper

Cited by 28 publications

References 24 publications

The significance of copper chelators in clinical and experimental application

The significance of copper chelators in clinical and experimental application

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

The Effects of Temperature Variation on the Sensitivity to Pesticides: a Study on the Slime Mould Dictyostelium discoideum (Protozoa)

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