Occurrence of echinocytosis in circulating RBC of black bullhead, Ictalurus melas (Rafinesque), following exposure to an anionic detergent at sublethal concentrations

Zeni, Cristina; Bovolenta, Maria Rita; Stagni, Annamaria

doi:10.1016/s0166-445x(01)00203-x

Cited by 19 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results have also been reported by Massar et al (2012). Abnormally shaped red blood cells, codocytes observed in the current study has been reported by Naskar et al, 2006. Zeni et al (2002 in a study on fish Ictalurus melas observed occurrence of echinocytosis when exposed to sublethal concentrations of an anionic detergent.…”

Section: Discussionsupporting

confidence: 86%

Untitled

Maheshwari¹,

Dua²

2016

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Fish are susceptible to harmful effects of heavy metals and other effluents which influence the hematological profile. Hematological parameters are considered as pathophysiological indicators and are important in diagnosing the structural and functional status of fish exposed to toxicants. Ultrastructural analyses can be used to study the alterations in erythrocytes due to environmental stress. The aim of the present study was to evaluate the toxicity of mercuric chloride on erythrocytes of fish, Channa punctatus. The experiments were conducted in accordance with the standard methods given in APHA (2005) and EPA (1996). Erythrocytes of the fish are nucleated and elliptical in shape. Scanning electron micrographs revealed the presence of spherocytes, crenation of membrane, membrane internalization and development of lobopodial projections in the erythrocytes of exposed fish. The study substantiates the hypothesis that assessment of morphological damage to erythrocytes through scanning electron microscopy can be effectively used as a toxicological tool.

show abstract

Section: Discussionsupporting

confidence: 86%

Untitled

Maheshwari¹,

Dua²

2016

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

“…Morphological changes in erythrocytes often occur in fish as a result of intoxication with various inorganic or organic poisons (Sorensen and Bauer 1983;Fletcher and White 1986;Gill and Pant 1987;Shandilya and Banerjee 1989;Boge and Roche 1996;Vosyliene 1996;Zeni et al 2002). According to Vosyliene (1999) and Zeni et al (2002), morphology of erythrocytes is one of the most specific and sensitive indicators of the effect of various environmental factors on fish. Own data (Witeska 2001(Witeska , 2003 obtained for common carp subjected to 10 mg dm )3 of Cd for 3 h showed also considerable morphological anomalies in these cells (mainly nuclear malformations, cell malformations, and divisions).…”

Section: Discussionmentioning

confidence: 99%

Respiratory and hematological response of tench, Tinca tinca (L.) to a short-term cadmium exposure

Witeska¹,

Jezierska²,

Wolnicki

2005

Aquacult Int

View full text Add to dashboard Cite

The effects of 3 h exposure to 96hLC50 of cadmium (4.5 mg dm )3 ) on oxygen consumption rate, and hematological parameters (RBC, WBC, erythrocyte and leukocyte pattern) of juvenile tench were evaluated. Oxygen consumption significantly decreased beginning from 24 h postexposure, and remained reduced until the end of the experiment (96 h postexposure). RBC gradually increased, together with the percentage of juvenile cells in circulation. On the other hand, cadmium induced damage to the red cells -the share of cellular anomalies significantly increased with time postexposure. They included abnormal cell shape, vacuolization, swelling, chromatin disintegration in the nucleus, and nucleus indentation. The exposed fish showed a gradual and significant decrease in WBC without a shift of lymphocyte/neutrophil proportion. No significant changes in thrombocyte count occurred. The results show that short-term exposure to cadmium reduced fish energetic metabolism, and suppressed immune abilities. The symptoms gradually developed after the end of exposure, and no recovery took place until 96 h.

show abstract

“…Inwardly the multiplicity of biomarkers for evaluating aquatic environments, those which mirror effects on DNA, are well known due to ecological implications (Ramsdorf et al, 2012). Erythrocyte morphology of fishes is more sensitive to various environmental contaminants than red blood cell parameters (Witeska, 2004;Zeni, Bovolenta, & Stagni, 2002), and cellular abnormalities are sometimes observed without distinct decrease in their values (Witeska, Kondera, Szymanska, & Ostrysz, 2010). The micronucleus test is one of the most utilized cytogenetic techniques for genotoxicity assays and has been widely applied in peripheral blood of teleostean fishes in studies in the field (in situ) (Tolbert, Shy, & Allen, 1991) and bioassays (Al-Sabti & Metcalfe, 1995).…”

Section: Introductionmentioning

confidence: 99%

Digital light microscopy as a tool in toxicological evaluation of fish erythrocyte morphological abnormalities

Sula

Aliko

Pagano

et al. 2019

Microscopy Res & Technique

View full text Add to dashboard Cite

Fish erythrocytes are very sensitive to environmental pollution because they exhibit pathological alterations before appearance of other external symptoms, may be used as reliable toxicity bioindicators. Microscopic analysis can be a useful tool in early detection of alterations in erythrocyte morphology due to different environmental stressors, and in predicting organism's possible response ways. This study aims to evaluate the toxicity of a polluted freshwater lake on the erythrocytes of crucian carp fish, Carassius carassius, using digital light microscopy (DLM). Scanning DLM micrographs revealed the presence of nuclear and cytoplasmic abnormalities like micronucleus, nuclear buds, lobed nucleus, deformed nucleus, vacuolated cytoplasm, and echinocyte in the erythrocytes of crucian carp fish from a polluted lake. The obtained results substantiate that computation of erythrocyte morphological damages as biomarkers of exposure, through digital light microscopy software, can be effectively used as a toxicological tool.

show abstract

Occurrence of echinocytosis in circulating RBC of black bullhead, Ictalurus melas (Rafinesque), following exposure to an anionic detergent at sublethal concentrations

Cited by 19 publications

References 52 publications

Untitled

Untitled

Respiratory and hematological response of tench, Tinca tinca (L.) to a short-term cadmium exposure

Digital light microscopy as a tool in toxicological evaluation of fish erythrocyte morphological abnormalities

Contact Info

Product

Resources

About